دورية أكاديمية
Lrp13a and Lrp13b serve as vitellogenin receptors in the ovary of zebrafish†.
| العنوان: | Lrp13a and Lrp13b serve as vitellogenin receptors in the ovary of zebrafish†. |
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| المؤلفون: | Liu Z; College of Life Sciences, Northwest Normal University, Lanzhou, China., Zhang N; College of Life Sciences, Northwest Normal University, Lanzhou, China., Wang C; College of Life Sciences, Northwest Normal University, Lanzhou, China., Shi L; College of Life Sciences, Northwest Normal University, Lanzhou, China., Hu Y; College of Life Sciences, Northwest Normal University, Lanzhou, China., Wang Y; College of Life Sciences, Northwest Normal University, Lanzhou, China., Li J; College of Life Sciences, Northwest Normal University, Lanzhou, China. |
| المصدر: | Biology of reproduction [Biol Reprod] 2024 Jul 12; Vol. 111 (1), pp. 123-134. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0207224 Publication Model: Print Cited Medium: Internet ISSN: 1529-7268 (Electronic) Linking ISSN: 00063363 NLM ISO Abbreviation: Biol Reprod Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2017- : New York : Oxford University Press Original Publication: Champaign, Ill. : Society for the Study of Reproduction |
| مواضيع طبية MeSH: | Zebrafish* , Zebrafish Proteins*/genetics , Zebrafish Proteins*/metabolism , Ovary*/metabolism , Vitellogenins*/metabolism , Vitellogenins*/genetics , Egg Proteins*/metabolism , Egg Proteins*/genetics, Animals ; Female ; Receptors, Cell Surface/metabolism ; Receptors, Cell Surface/genetics |
| مستخلص: | In oviparous animals, egg yolk is largely derived from vitellogenin, which is taken up from the maternal circulation by the growing oocytes via the vitellogenin receptor. Recently, a novel member of the lipoprotein receptor superfamily termed low-density lipoprotein receptor-related protein 13 was identified and proposed as a candidate of vitellogenin receptor in oviparous animals. However, the roles of low-density lipoprotein receptor-related protein 13 in vitellogenesis are still poorly defined. Here, we investigated the expression, vitellogenin-binding properties, and function of low-density lipoprotein receptor-related protein 13 in zebrafish. Two different lrp13 genes termed lrp13a and lrp13b were found in zebrafish. Reverse transcription polymerase chain reaction and quantitative polymerase chain reaction revealed both lrp13s to be predominantly expressed in zebrafish ovary, and in situ hybridization detected both lrp13s transcripts in the ooplasm of early stage oocytes. Two yeast hybrid studies showed that among eight vitellogenins of zebrafish, Vtg1, 2, and 3 bind to Lrp13a, while Vtg1, 2, and 5 bind to Lrp13b. We created zebrafish lrp13a and lrp13b mutant lines using CRISPR/Cas9. Knockout of lrp13a leads to a male-biased sex ratio and decreased diameter of embryo yolk, while knockout of lrp13b and double knockout of lrp13a and lrp13b leads to the delay of vitellogenesis, followed by follicular atresia. These phenotypes of mutants can be explained by the disruption of vitellogenesis in the absence of Lrp13s. Taken together, our results indicate that both Lrp13a and Lrp13b can serve as vitellogenin receptors in zebrafish among other vitellogenin receptors that are not yet described. (© The Author(s) 2024. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| معلومات مُعتمدة: | 22JR5RA148 Natural Science Foundation of Gansu Province; 32060170 National Natural Science Foundation of China |
| فهرسة مساهمة: | Keywords: Lrp13; ovary; vitellogenesis; vitellogenin; zebrafish |
| المشرفين على المادة: | 0 (vitellogenin receptor) 0 (Zebrafish Proteins) 0 (Vitellogenins) 0 (Egg Proteins) 0 (Receptors, Cell Surface) |
| تواريخ الأحداث: | Date Created: 20240425 Date Completed: 20240715 Latest Revision: 20240715 |
| رمز التحديث: | 20240715 |
| DOI: | 10.1093/biolre/ioae040 |
| PMID: | 38660750 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1529-7268 |
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| DOI: | 10.1093/biolre/ioae040 |