دورية أكاديمية
Functional single-cell analyses of mesenchymal stromal cell proliferation and differentiation using ALDH-activity and mitochondrial ROS content.
العنوان: | Functional single-cell analyses of mesenchymal stromal cell proliferation and differentiation using ALDH-activity and mitochondrial ROS content. |
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المؤلفون: | Refeyton A; Etablissement Français du Sang Nouvelle Aquitaine, Bordeaux, France; Université de Bordeaux, Bordeaux, France; Inserm Bordeaux U1211, Bordeaux, France., Labat V; Etablissement Français du Sang Nouvelle Aquitaine, Bordeaux, France; Université de Bordeaux, Bordeaux, France; Inserm Bordeaux U1211, Bordeaux, France., Mombled M; Etablissement Français du Sang Nouvelle Aquitaine, Bordeaux, France; Genethon, Évry-Courcouronne, France; Inserm, Évry-Courcouronne, France., Vlaski-Lafarge M; Etablissement Français du Sang Nouvelle Aquitaine, Bordeaux, France; Université de Bordeaux, Bordeaux, France; Inserm Bordeaux U1211, Bordeaux, France., Ivanovic Z; Etablissement Français du Sang Nouvelle Aquitaine, Bordeaux, France; Université de Bordeaux, Bordeaux, France; Inserm Bordeaux U1211, Bordeaux, France. Electronic address: zoran.ivanovic@efs.sante.fr. |
المصدر: | Cytotherapy [Cytotherapy] 2024 Aug; Vol. 26 (8), pp. 813-824. Date of Electronic Publication: 2024 Apr 10. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 100895309 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-2566 (Electronic) Linking ISSN: 14653249 NLM ISO Abbreviation: Cytotherapy Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2013- : London : Elsevier Original Publication: Oxford, England : ISIS Medical Media, c1999- |
مواضيع طبية MeSH: | Mesenchymal Stem Cells*/metabolism , Mesenchymal Stem Cells*/cytology , Cell Differentiation* , Cell Proliferation* , Aldehyde Dehydrogenase*/metabolism , Reactive Oxygen Species*/metabolism , Mitochondria*/metabolism , Single-Cell Analysis*/methods, Humans ; Cells, Cultured |
مستخلص: | Baskground: Previous research has unveiled a stem cell-like transcriptome enrichment in the aldehyde dehydrogenase-expressing (ALDH high ) mesenchymal stromal cell (MStroC) fraction. However, considering the heterogeneity of MStroCs, with only a fraction of them presenting bona fide stem cells (MSCs), the actual potency of ALDH as an MSC-specific selection marker remains an issue. Methods: To address this, the proliferative and differentiation potential of individual ALDH high and ALDH low MStroCs incubated at low oxygen concentrations, estimated to mimic stem cell niches (0.1% O Results: We confirm that a high proliferative capacity and multi-potent MSCs are enriched in the ALDH high MStroC population, especially when cells are cultured at 0.1% O Conclusion: Our study reveals that ALDH expression is not exclusively associated with MSCs. However, cell sorting using combined ALDH expression and ROS content can be utilized to exclude MStroCs lacking stem/progenitor cell properties. Competing Interests: Declaration of Competing Interest The authors have no commercial, proprietary or financial interest in the products or companies described in this article. (Copyright © 2024 International Society for Cell & Gene Therapy. Published by Elsevier Inc. All rights reserved.) |
فهرسة مساهمة: | Keywords: ROS content; aldehyde dehydrogenase; mesenchymal stem cells; multi-potency; oxygen concentration; single cell analysis |
المشرفين على المادة: | EC 1.2.1.3 (Aldehyde Dehydrogenase) 0 (Reactive Oxygen Species) |
تواريخ الأحداث: | Date Created: 20240425 Date Completed: 20240725 Latest Revision: 20240725 |
رمز التحديث: | 20240726 |
DOI: | 10.1016/j.jcyt.2024.04.003 |
PMID: | 38661612 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1477-2566 |
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DOI: | 10.1016/j.jcyt.2024.04.003 |