دورية أكاديمية
أعرض في EDS

Functional single-cell analyses of mesenchymal stromal cell proliferation and differentiation using ALDH-activity and mitochondrial ROS content.

التفاصيل البيبلوغرافية
العنوان: Functional single-cell analyses of mesenchymal stromal cell proliferation and differentiation using ALDH-activity and mitochondrial ROS content.
المؤلفون: Refeyton A; Etablissement Français du Sang Nouvelle Aquitaine, Bordeaux, France; Université de Bordeaux, Bordeaux, France; Inserm Bordeaux U1211, Bordeaux, France., Labat V; Etablissement Français du Sang Nouvelle Aquitaine, Bordeaux, France; Université de Bordeaux, Bordeaux, France; Inserm Bordeaux U1211, Bordeaux, France., Mombled M; Etablissement Français du Sang Nouvelle Aquitaine, Bordeaux, France; Genethon, Évry-Courcouronne, France; Inserm, Évry-Courcouronne, France., Vlaski-Lafarge M; Etablissement Français du Sang Nouvelle Aquitaine, Bordeaux, France; Université de Bordeaux, Bordeaux, France; Inserm Bordeaux U1211, Bordeaux, France., Ivanovic Z; Etablissement Français du Sang Nouvelle Aquitaine, Bordeaux, France; Université de Bordeaux, Bordeaux, France; Inserm Bordeaux U1211, Bordeaux, France. Electronic address: zoran.ivanovic@efs.sante.fr.
المصدر: Cytotherapy [Cytotherapy] 2024 Aug; Vol. 26 (8), pp. 813-824. Date of Electronic Publication: 2024 Apr 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: England NLM ID: 100895309 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-2566 (Electronic) Linking ISSN: 14653249 NLM ISO Abbreviation: Cytotherapy Subsets: MEDLINE
أسماء مطبوعة: Publication: 2013- : London : Elsevier
Original Publication: Oxford, England : ISIS Medical Media, c1999-
مواضيع طبية MeSH: Mesenchymal Stem Cells*/metabolism , Mesenchymal Stem Cells*/cytology , Cell Differentiation* , Cell Proliferation* , Aldehyde Dehydrogenase*/metabolism , Reactive Oxygen Species*/metabolism , Mitochondria*/metabolism , Single-Cell Analysis*/methods, Humans ; Cells, Cultured
مستخلص: Baskground: Previous research has unveiled a stem cell-like transcriptome enrichment in the aldehyde dehydrogenase-expressing (ALDH high ) mesenchymal stromal cell (MStroC) fraction. However, considering the heterogeneity of MStroCs, with only a fraction of them presenting bona fide stem cells (MSCs), the actual potency of ALDH as an MSC-specific selection marker remains an issue.
Methods: To address this, the proliferative and differentiation potential of individual ALDH high and ALDH low MStroCs incubated at low oxygen concentrations, estimated to mimic stem cell niches (0.1% O 2 ), were assayed using single-cell clonal analysis, compared to standard conditions (20% O 2 ).
Results: We confirm that a high proliferative capacity and multi-potent MSCs are enriched in the ALDH high MStroC population, especially when cells are cultured at 0.1% O 2 . Measurements of reduced/oxidized glutathione and mitochondrial superoxide anions with MitoSoX (MSX) indicate that this advantage induced by low oxygen is related to a decrease in the oxidative and reactive oxygen species (ROS) levels in the stem cell metabolic setup. However, ALDH expression is neither specific nor exclusive to MSCs, as high proliferative capacity and multi-potent cells were also found in the ALDH low fraction. Furthermore, single-cell assays performed after combined cell sorting based on ALDH and MSX showed that the MSX low MStroC population is enriched in stem/progenitor cells in all conditions, irrespective of ALDH expression or culture oxygen concentration. Importantly, the ALDH high MSX low MStroC fraction exposed to 0.1% O 2 was almost exclusively composed of genuine MSCs. In contrast, neither progenitors nor stem cells (with a complete absence of colony-forming ability) were detected in the MSX high fraction, which exclusively resides in the ALDH low MStroC population.
Conclusion: Our study reveals that ALDH expression is not exclusively associated with MSCs. However, cell sorting using combined ALDH expression and ROS content can be utilized to exclude MStroCs lacking stem/progenitor cell properties.
Competing Interests: Declaration of Competing Interest The authors have no commercial, proprietary or financial interest in the products or companies described in this article.
(Copyright © 2024 International Society for Cell & Gene Therapy. Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: ROS content; aldehyde dehydrogenase; mesenchymal stem cells; multi-potency; oxygen concentration; single cell analysis
المشرفين على المادة: EC 1.2.1.3 (Aldehyde Dehydrogenase)
0 (Reactive Oxygen Species)
تواريخ الأحداث: Date Created: 20240425 Date Completed: 20240725 Latest Revision: 20240725
رمز التحديث: 20240726
DOI: 10.1016/j.jcyt.2024.04.003
PMID: 38661612
قاعدة البيانات: MEDLINE
الوصف
تدمد:1477-2566
DOI:10.1016/j.jcyt.2024.04.003