دورية أكاديمية
Lysosomal diacylglycerol pyrophosphate phosphatase is not essential in Trypanosoma brucei.
| العنوان: | Lysosomal diacylglycerol pyrophosphate phosphatase is not essential in Trypanosoma brucei. |
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| المؤلفون: | Dawoody Nejad L; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, BC, Canada. ladan.dawoody1@gmail.com.; Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland. ladan.dawoody1@gmail.com.; Graduate School for Cellular and Biochemical Sciences, University of Bern, Bern, Switzerland. ladan.dawoody1@gmail.com., Annese T; Department of Translational Biomedicine and Neuroscience, University of Bari, Bari, Italy.; Department of Medicine and Surgery, Libera Università del Mediterraneo (LUM) Giuseppe Degennaro, Bari, Italy., Ribatti D; Department of Translational Biomedicine and Neuroscience, University of Bari, Bari, Italy. |
| المصدر: | Molecular biology reports [Mol Biol Rep] 2024 Apr 26; Vol. 51 (1), pp. 578. Date of Electronic Publication: 2024 Apr 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Dordrecht, Boston, Reidel. |
| مواضيع طبية MeSH: | Trypanosoma brucei brucei*/enzymology , Trypanosoma brucei brucei*/genetics , Lysosomes*/metabolism , Lysosomes*/enzymology, Glycerol/*analogs & derivatives, Triglycerides/metabolism ; Protozoan Proteins/metabolism ; Protozoan Proteins/genetics ; Phosphatidate Phosphatase/metabolism ; Phosphatidate Phosphatase/genetics ; RNA Interference ; Diphosphates/metabolism ; Phosphoric Monoester Hydrolases/metabolism ; Phosphoric Monoester Hydrolases/genetics ; Diglycerides/metabolism ; Phosphatidic Acids/metabolism |
| مستخلص: | Mg 2+ -independent phosphatidic acid phosphatase (PAP2), diacylglycerol pyrophosphate phosphatase 1 (Dpp1) is a membrane-associated enzyme in Saccharomyces cerevisiae. The enzyme is responsible for inducing the breakdown of β-phosphate from diacylglycerol pyrophosphate (DGPP) into phosphatidate (PA) and then removes the phosphate from PA to give diacylglycerol (DAG). In this study through RNAi suppression, we have demonstrated that Trypanosoma brucei diacylglycerol pyrophosphate phosphatase 1 (TbDpp1) procyclic form production is not required for parasite survival in culture. The steady-state levels of triacylglycerol (TAG), the number of lipid droplets, and the PA content are all maintained constant through the inducible down-regulation of TbDpp1. Furthermore, the localization of C-terminally tagged variants of TbDpp1 in the lysosome was demonstrated by immunofluorescence microscopy. (© 2024. Crown.) |
| References: | Dawoody Nejad L, Serricchio M, Jelk J et al (2018) TbLpn, a key enzyme in lipid droplet formation and phospholipid metabolism, is essential for mitochondrial integrity and growth of Trypanosoma Brucei. Mol Microbiol. https://doi.org/10.1111/mmi.13976. (PMID: 10.1111/mmi.1397629679486) Carman GM, Han GS (2006) Roles of phosphatidate phosphatase enzymes in lipid metabolism. Trends Biochem Sci 31:694–699. (PMID: 10.1016/j.tibs.2006.10.00317079146) Péterfy M, Phan J, Xu P, Reue K (2001) Lipodystrophy in the fld mouse results from mutation of a new gene encoding a nuclear protein, lipin. Nat Genet. https://doi.org/10.1038/83685. (PMID: 10.1038/8368511138012) Nanjundan M, Possmayer F (2003) Pulmonary phosphatidic acid phosphatase and lipid phosphate phosphohydrolase. Am. J. Physiol. - Lung Cell. Mol. Physiol. Brindley DN, Pilquil C (2009) Lipid phosphate phosphatases and signaling. J Lipid Res. https://doi.org/10.1194/jlr.r800055-jlr200. (PMID: 10.1194/jlr.r800055-jlr20019201733) Toke DA, Bennett WL, Dillon DA et al Isolation and characterization of the Saccharomyces cerevisiae DPP1 Gene Encoding Diacylglycerol Pyrophosphate phosphatase *. 3278–3285. Toke DA, Bennett WL, Oshiro J et al (1998) Isolation and characterization of the Saccharomyces cerevisiae LPP1 gene encoding a Mg2+-independent phosphatidate phosphatase. J Biol Chem. https://doi.org/10.1074/jbc.273.23.14331. (PMID: 10.1074/jbc.273.23.143319603941) Hardman D, McFalls D, Fakas S (2017) Characterization of phosphatidic acid phosphatase activity in the oleaginous yeast Yarrowia Lipolytica and its role in lipid biosynthesis. https://doi.org/10.1002/yea.3216 . Yeast. Han GS, Johnston CN, Carman GM (2004) Vacuole membrane topography of the DPP1-encoded Diacylglycerol Pyrophosphate Phosphatase Catalytic Site from Saccharomyces cerevisiae. J Biol Chem. https://doi.org/10.1074/jbc.M311779200. (PMID: 10.1074/jbc.M31177920015561725) Huh WK, Falvo JV, Gerke LC et al (2003) Global analysis of protein localization in budding yeast. Nature. https://doi.org/10.1038/nature02026. (PMID: 10.1038/nature0202614562106) Carman GM, Han GS (2007) Regulation of phospholipid synthesis in Saccharomyces cerevisiae by zinc depletion. Biochim. Biophys. Acta - Mol. Cell Biol. Lipids. Riedel B, Morr M, Wu WI et al (1997) Metabolism of diacylglycerol pyrophosphate by suspension cultured Catharanthus roseus cells identification and characterization of diacylglycerol pyrophosphate phosphatase in plants. Plant Sci 128:1–10. https://doi.org/10.1016/S0168-9452(97)00120-9. (PMID: 10.1016/S0168-9452(97)00120-9) English D, Cui Y, Siddiqui RA (1996) Messenger functions of phosphatidic acid. Chem Phys Lipids. https://doi.org/10.1016/0009-3084(96)02549-2. (PMID: 10.1016/0009-3084(96)02549-28681423) Franco JR, Simarro PP, Diarra A, Jannin JG (2014) Epidemiology of human African trypanosomiasis. Clin Epidemiol 6:257–275. (PMID: 25125985) Wirtz E, Leal S, Ochatt C, Cross GAM (1999) A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma Brucei. 99:89–101. Dawoody Nejad L, Stumpe M, Rauch M et al (2020) Mitochondrial sphingosine-1-phosphate lyase is essential for phosphatidylethanolamine synthesis and survival of Trypanosoma Brucei. Sci Rep. https://doi.org/10.1038/s41598-020-65248-x. (PMID: 10.1038/s41598-020-65248-x32427974) Niemann M, Wiese S, Mani J et al (2013) Mitochondrial outer membrane proteome of trypanosoma brucei reveals novel factors required to maintain mitochondrial morphology. Mol Cell Proteom. https://doi.org/10.1074/mcp.M112.023093. (PMID: 10.1074/mcp.M112.023093) Rao X, Huang X, Zhou Z, Lin X (2013) An improvement of the 2ˆ(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. Biostat Bioinforma Biomath. Oberholzer M, Morand S, Kunz S, Seebeck T (2006) A vector series for rapid PCR-mediated C-terminal in situ tagging of Trypanosoma brucei genes. Mol Biochem Parasitol. https://doi.org/10.1016/j.molbiopara.2005.09.002. (PMID: 10.1016/j.molbiopara.2005.09.00216280178) Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917. https://doi.org/10.1139/o59-099. (PMID: 10.1139/o59-09913671378) Käll L, Krogh A, Sonnhammer ELL (2007) Advantages of combined transmembrane topology and signal peptide prediction-the Phobius web server. Nucleic Acids Res 35. https://doi.org/10.1093/nar/gkm256. Sonnhammer EL, von Heijne G, Krogh A (1998) A hidden Markov model for predicting transmembrane helices in protein sequences. Proc Int Conf Intell Syst Mol Biol. Allmann S, Mazet M, Ziebart N et al (2014) Triacylglycerol storage in lipid droplets in procyclic trypanosoma brucei. PLoS ONE. https://doi.org/10.1371/journal.pone.0114628. (PMID: 10.1371/journal.pone.011462825493940) Wu WI, Liu Y, Riedel B et al (1996) Purification and characterization of diacylglycerol pyrophosphate phosphatase from Saccharomyces cerevisiae. J Biol Chem. https://doi.org/10.1074/jbc.271.4.1868. (PMID: 10.1074/jbc.271.4.18688955109) Oshiro J, Han GS, Carman GM (2003) Diacylglycerol pyrophosphate phosphatase in Saccharomyces cerevisiae. Biochim. Biophys. Acta - Mol. Cell Biol. Lipids. Wissing JB, Kornak B, Funke A, Riedel B (1994) Phosphatidate kinase, a novel enzyme in phospholipid metabolism: characterization of the enzyme from suspension-cultured Catharanthus roseus cells. Plant Physiol. https://doi.org/10.1104/pp.105.3.903. (PMID: 10.1104/pp.105.3.90312232252) Dillon DA, Wut WI, Riedel B et al (1996) The Escherichia coli pgpB gene encodes for a diacylglycerol pyrophosphate phosphatase activity. J Biol Chem. https://doi.org/10.1074/jbc.271.48.30548. (PMID: 10.1074/jbc.271.48.305488955126) Dillon DA, Chen X, Zeimetz GM et al (1997) Mammalian Mg2+-independent phosphatidate phosphatase (PAP2) displays diacylglycerol pyrophosphate phosphatase activity. J Biol Chem. https://doi.org/10.1074/jbc.272.16.10361. (PMID: 10.1074/jbc.272.16.103619099673) van Schooten B, Testerink C, Munnik T (2006) Signalling diacylglycerol pyrophosphate, a new phosphatidic acid metabolite. Biochim. Biophys. Acta - Mol. Cell Biol. Lipids. Marchesini N, Santander V, Machado-Domenech E (1998) Diacylglycerol pyrophosphate: a novel metabolite in the Trypanosoma Cruzi phosphatidic acid metabolism. FEBS Lett. https://doi.org/10.1016/S0014-5793(98)01169-7. (PMID: 10.1016/S0014-5793(98)01169-79801152) Hiller C, Nissen A, Benítez D et al (2014) Cytosolic peroxidases protect the lysosome of Bloodstream African trypanosomes from Iron-mediated membrane damage. PLoS Pathog. https://doi.org/10.1371/journal.ppat.1004075. (PMID: 10.1371/journal.ppat.100407524945925) |
| معلومات مُعتمدة: | 31003A-169355 the Swiss National Science Foundation grants |
| فهرسة مساهمة: | Keywords: Trypanosoma brucei; Diacylglycerol pyrophosphate phosphatase; Lysosome; TbDpp1; Triacylglycerol |
| المشرفين على المادة: | 0 (Triglycerides) 0 (Protozoan Proteins) 0 (diacylglycerol pyrophosphate) EC 3.1.3.4 (Phosphatidate Phosphatase) 0 (Diphosphates) EC 3.1.3.2 (Phosphoric Monoester Hydrolases) 0 (Diglycerides) 0 (Phosphatidic Acids) PDC6A3C0OX (Glycerol) |
| تواريخ الأحداث: | Date Created: 20240426 Date Completed: 20240426 Latest Revision: 20240426 |
| رمز التحديث: | 20240426 |
| DOI: | 10.1007/s11033-024-09547-w |
| PMID: | 38668789 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1573-4978 |
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| DOI: | 10.1007/s11033-024-09547-w |