دورية أكاديمية
Engineering the β-galactosidase from Aspergillus oryzae for making lactose-free and no-sugar-added yogurt.
| العنوان: | Engineering the β-galactosidase from Aspergillus oryzae for making lactose-free and no-sugar-added yogurt. |
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| المؤلفون: | Miao M; Key Laboratory of China National Light Industry and Food Bioengineering, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China., Li S; Key Laboratory of China National Light Industry and Food Bioengineering, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Mengniu Hi-tech Dairy Product Beijing Co., Ltd., Beijing 101100, China., Yang S; Key Laboratory of China National Light Industry and Food Bioengineering, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China., Yan Q; College of Engineering, China Agricultural University, Beijing 100083, China., Xiang Z; Key Laboratory of China National Light Industry and Food Bioengineering, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China., Jiang Z; Key Laboratory of China National Light Industry and Food Bioengineering, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Food Laboratory of Zhongyuan, Luohe 462300, China. Electronic address: zhqjiang@cau.edu.cn. |
| المصدر: | Journal of dairy science [J Dairy Sci] 2024 Sep; Vol. 107 (9), pp. 6602-6613. Date of Electronic Publication: 2024 Apr 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Dairy Science Association Country of Publication: United States NLM ID: 2985126R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-3198 (Electronic) Linking ISSN: 00220302 NLM ISO Abbreviation: J Dairy Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Champaign, IL : American Dairy Science Association Original Publication: Lancaster, Pa. [etc.] |
| مواضيع طبية MeSH: | Yogurt* , Aspergillus oryzae*/enzymology , Aspergillus oryzae*/genetics , beta-Galactosidase*/metabolism , beta-Galactosidase*/genetics , Lactose*/metabolism, Hydrogen-Ion Concentration ; Fermentation |
| مستخلص: | Yogurt usually contains 5% to 7% sugar and 3% to 5% lactose. As β-galactosidases can hydrolyze lactose and improve sweetness, they have the potential to produce lactose-free (LF) and no-sugar-added (NSA) yogurt. In this study, the β-galactosidase AoBgal35A from Aspergillus oryzae was engineered by site-saturation mutagenesis. Results of 19 variants of T955 residue showed that the lactose hydrolysis rate of T955R-AoBgal35A was up to 90.7%, which is much higher than the 78.5% of the wild type. Moreover, the optimal pH of T955R-AoBgal35A was shifted from pH 4.5 to pH 5.5, and the optimal temperature decreased from 60°C to 50°C. The mutant T955R-AoBgal35A was successfully expressed in Komagataella pastoris, which produced extracellularly 4,528 U/mL of β-galactosidase activity. The mutant T955R-AoBgal35A was used to produce LF yogurt. The Streptococcus thermophilus count of LF yogurt increased from 7.9 to 9.5 log cfu/g, which is significantly higher than that of the control group (8.9 log cfu/g). The residual lactose content of LF yogurt was 0.13%, meeting the requirements of the national standard in China for the "lactose-free" label (<0.5%). Furthermore, sugar in yogurt was replaced by whey powder to produce LF-NSA yogurt. The optimal addition content of whey powder was 7.5%. The texture, water-holding capacity, and titratable acidity of LF and LF-NSA yogurt achieved good shelf life stability. Therefore, this study provides an insight for technological implications of β-galactosidases in the dairy industry. (The Authors. Published by Elsevier Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).) |
| فهرسة مساهمة: | Keywords: lactose-free yogurt; no-sugar-added yogurt; saturation mutagenesis; β-galactosidase |
| المشرفين على المادة: | EC 3.2.1.23 (beta-Galactosidase) J2B2A4N98G (Lactose) |
| تواريخ الأحداث: | Date Created: 20240426 Date Completed: 20240816 Latest Revision: 20240819 |
| رمز التحديث: | 20240819 |
| DOI: | 10.3168/jds.2023-24310 |
| PMID: | 38670341 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1525-3198 |
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| DOI: | 10.3168/jds.2023-24310 |