دورية أكاديمية
Hydrogel loaded with thiolated chitosan modified taxifolin liposome promotes osteoblast proliferation and regulates Wnt signaling pathway to repair rat skull defects.
| العنوان: | Hydrogel loaded with thiolated chitosan modified taxifolin liposome promotes osteoblast proliferation and regulates Wnt signaling pathway to repair rat skull defects. |
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| المؤلفون: | Ding Q; Jilin Agricultural University, Changchun 130118, China., Liu W; School of Food and Pharmaceutical Engineering, Wuzhou University, Wuzhou 543002, China., Zhang S; Jilin Agricultural University, Changchun 130118, China., Sun S; Jilin Agricultural University, Changchun 130118, China., Yang J; Jilin Agricultural University, Changchun 130118, China., Zhang L; Jilin Agricultural University, Changchun 130118, China., Wang N; Jilin Agricultural University, Changchun 130118, China., Ma S; Jilin Agricultural University, Changchun 130118, China., Chai G; Jilin Agricultural University, Changchun 130118, China., Shen L; Jilin Jianwei Natural Biotechnology Co., Ltd., Linjiang 134600, China., Gao Y; Jilin Jianwei Natural Biotechnology Co., Ltd., Linjiang 134600, China., Ding C; Jilin Agricultural University, Changchun 130118, China; College of Traditional Chinese Medicine, Jilin Agriculture Science and Technology College, Jilin 132101, China; Jilin Aodong Yanbian Pharmaceutical Co., Ltd, Yanbian Korean Autonomous Prefecture 133000, China. Electronic address: chuanboding0506@163.com., Liu X; College of Traditional Chinese Medicine, Jilin Agriculture Science and Technology College, Jilin 132101, China. Electronic address: xinglongliu1221@126.com. |
| المصدر: | Carbohydrate polymers [Carbohydr Polym] 2024 Jul 15; Vol. 336, pp. 122115. Date of Electronic Publication: 2024 Apr 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 8307156 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1344 (Electronic) Linking ISSN: 01448617 NLM ISO Abbreviation: Carbohydr Polym Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1992-> : Barking : Elsevier Applied Science Publishers Original Publication: London [Eng.] : Applied Science Publishers, c1981- |
| مواضيع طبية MeSH: | Quercetin*/*analogs & derivatives, Chitosan*/analogs & derivatives , Chitosan*/chemistry , Chitosan*/pharmacology , Quercetin*/pharmacology , Quercetin*/chemistry , Liposomes*/chemistry , Wnt Signaling Pathway*/drug effects , Osteoblasts*/drug effects , Hydrogels*/chemistry , Hydrogels*/pharmacology , Cell Proliferation*/drug effects , Skull*/drug effects , Skull*/pathology , Skull*/metabolism, Animals ; Mice ; Rats ; Bone Regeneration/drug effects ; Rats, Sprague-Dawley ; Osteogenesis/drug effects ; Staphylococcus aureus/drug effects ; Sulfhydryl Compounds/chemistry ; Sulfhydryl Compounds/pharmacology ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/chemistry ; Cell Differentiation/drug effects ; Escherichia coli/drug effects ; Male ; Molecular Docking Simulation |
| مستخلص: | To alleviate skull defects and enhance the biological activity of taxifolin, this study utilized the thin-film dispersion method to prepare paclitaxel liposomes (TL). Thiolated chitosan (CSSH)-modified TL (CTL) was synthesized through charge interactions. Injectable hydrogels (BLG) were then prepared as hydrogel scaffolds loaded with TAX (TG), TL (TLG), and CTL (CTLG) using a Schiff base reaction involving oxidized dextran and carboxymethyl chitosan. The study investigated the bone reparative properties of CTLG through molecular docking, western blot techniques, and transcriptome analysis. The particle sizes of CTL were measured at 248.90 ± 14.03 nm, respectively, with zeta potentials of +36.68 ± 5.43 mV, respectively. CTLG showed excellent antioxidant capacity in vitro. It also has a good inhibitory effect on Escherichia coli and Staphylococcus aureus, with inhibition rates of 93.88 ± 1.59 % and 88.56 ± 2.83 % respectively. The results of 5-ethynyl-2 '-deoxyuridine staining, alkaline phosphatase staining and alizarin red staining showed that CTLG also had the potential to promote the proliferation and differentiation of mouse embryonic osteoblasts (MC3T3-E1). The study revealed that CTLG enhances the expression of osteogenic proteins by regulating the Wnt signaling pathway, shedding light on the potential application of TAX and bone regeneration mechanisms. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bone defects; Hydrogel scaffolds; Thiolated chitosan modified taxifolin liposomes |
| المشرفين على المادة: | 9012-76-4 (Chitosan) 9IKM0I5T1E (Quercetin) 0 (Liposomes) 0 (Hydrogels) 9SOB9E3987 (taxifolin) 0 (Sulfhydryl Compounds) 0 (Anti-Bacterial Agents) |
| تواريخ الأحداث: | Date Created: 20240426 Date Completed: 20240426 Latest Revision: 20240426 |
| رمز التحديث: | 20240427 |
| DOI: | 10.1016/j.carbpol.2024.122115 |
| PMID: | 38670750 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1344 |
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| DOI: | 10.1016/j.carbpol.2024.122115 |