دورية أكاديمية
Thermosensitive composite based on agarose and chitosan saturated with carbon dioxide. Preliminary study of requirements for production of new CSAG bioink.
| العنوان: | Thermosensitive composite based on agarose and chitosan saturated with carbon dioxide. Preliminary study of requirements for production of new CSAG bioink. |
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| المؤلفون: | Banach-Kopeć A; Department of Chemistry, Technology and Biotechnology of Food, Faculty of Chemistry, Advanced Materials Center, Gdańsk University of Technology, G. Narutowicza 11/12 Str., 80-233 Gdansk, Poland. Electronic address: adrianna.banach@pg.edu.pl., Mania S; Department of Chemistry, Technology and Biotechnology of Food, Faculty of Chemistry, Advanced Materials Center, Gdańsk University of Technology, G. Narutowicza 11/12 Str., 80-233 Gdansk, Poland. Electronic address: szymon.mania@pg.edu.pl., Tylingo R; Department of Chemistry, Technology and Biotechnology of Food, Faculty of Chemistry, Advanced Materials Center, Gdańsk University of Technology, G. Narutowicza 11/12 Str., 80-233 Gdansk, Poland. Electronic address: robertt@pg.edu.pl., Wawrzynowicz A; Department of Chemistry, Technology and Biotechnology of Food, Faculty of Chemistry, Advanced Materials Center, Gdańsk University of Technology, G. Narutowicza 11/12 Str., 80-233 Gdansk, Poland. Electronic address: s171385@student.pg.edu.pl., Pawłowska M; Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, G. Narutowicza 11/12 Str., 80-233 Gdansk, Poland. Electronic address: monika.pawlowska@pg.edu.pl., Czerwiec K; Division of Clinical Anatomy, Medical University of Gdańsk, M. Skłodowskiej-Curie 3a Str., 80-210 Gdańsk, Poland. Electronic address: katarzyna.czerwiec@gumed.edu.pl., Deptuła M; Laboratory of Tissue Engineering and Regenerative Medicine, Division of Embryology, Medical University of Gdansk, M. Skłodowskiej-Curie 3a Str., 80-210 Gdańsk, Poland. Electronic address: milena.deptula@gumed.edu.pl., Pikuła M; Laboratory of Tissue Engineering and Regenerative Medicine, Division of Embryology, Medical University of Gdansk, M. Skłodowskiej-Curie 3a Str., 80-210 Gdańsk, Poland. Electronic address: michal.pikula@gumed.edu.pl. |
| المصدر: | Carbohydrate polymers [Carbohydr Polym] 2024 Jul 15; Vol. 336, pp. 122120. Date of Electronic Publication: 2024 Apr 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 8307156 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1344 (Electronic) Linking ISSN: 01448617 NLM ISO Abbreviation: Carbohydr Polym Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1992-> : Barking : Elsevier Applied Science Publishers Original Publication: London [Eng.] : Applied Science Publishers, c1981- |
| مواضيع طبية MeSH: | Chitosan*/chemistry , Chitosan*/pharmacology , Carbon Dioxide*/chemistry , Sepharose*/chemistry , Hydrogels*/chemistry , Hydrogels*/pharmacology , Ink* , Escherichia coli*/drug effects, Humans ; Animals ; Temperature ; Staphylococcus aureus/drug effects ; Mice ; Rheology ; Cell Line ; Cell Proliferation/drug effects ; Tissue Engineering/methods ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/chemistry ; Fibroblasts/drug effects |
| مستخلص: | This study introduces a method for producing printable, thermosensitive bioink formulated from agarose (AG) and carbon dioxide-saturated chitosan (CS) hydrogels. The research identified medium molecular weight chitosan as optimal for bioink production, with a preferred chitosan hydrogel content of 40-60 %. Rheological analysis reveals the bioink's pseudoplastic behavior and a sol-gel phase transition between 27.0 and 31.5 °C. The MMW chitosan-based bioink showed also the most stable extrusion characteristic. The choice of chitosan for the production of bioink was also based on the assessment of the antimicrobial activity of the polymer as a function of its molecular weight and the degree of deacetylation, noting significant cell reduction rates for E. coli and S. aureus of 1.72 and 0.54 for optimal bioink composition, respectively. Cytotoxicity assessments via MTT and LDH tests confirm the bioink's safety for L929, HaCaT, and 46BR.1 N cell lines. Additionally, XTT proliferation assay proved the stimulating effect of the bioink on the proliferation of 46BR.1 N fibroblasts, comparable to that observed with Fetal Bovine Serum (FBS). FTIR spectroscopy confirms the bioink as a physical polymer blend. In conclusion, the CS/AG bioink demonstrates the promising potential for advanced spatial cell cultures in tissue engineering applications including skin regeneration. Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Szymon Mania reports financial support was provided by Gdansk University of Technology. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 3D printing, antimicrobial properties; Agarose; Bioink; Biosafety, chitosan |
| المشرفين على المادة: | 9012-76-4 (Chitosan) 142M471B3J (Carbon Dioxide) 9012-36-6 (Sepharose) 0 (Hydrogels) 0 (Anti-Bacterial Agents) |
| تواريخ الأحداث: | Date Created: 20240426 Date Completed: 20240426 Latest Revision: 20240426 |
| رمز التحديث: | 20240427 |
| DOI: | 10.1016/j.carbpol.2024.122120 |
| PMID: | 38670752 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1344 |
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| DOI: | 10.1016/j.carbpol.2024.122120 |