دورية أكاديمية
Metagenomic Analysis for Diagnosis of Hemorrhagic Fever in Minas Gerais, Brazil.
العنوان: | Metagenomic Analysis for Diagnosis of Hemorrhagic Fever in Minas Gerais, Brazil. |
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المؤلفون: | Iani FCM; Laboratory of Virology, Ezequiel Dias Foundation (FUNED), Belo Horizonte 30510-010, MG, Brazil., de Campos GM; Blood Center of Riberirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14051-140, SP, Brazil., Adelino TER; Laboratory of Virology, Ezequiel Dias Foundation (FUNED), Belo Horizonte 30510-010, MG, Brazil., da Silva AS; Laboratory of Virology, Ezequiel Dias Foundation (FUNED), Belo Horizonte 30510-010, MG, Brazil., Kashima S; Blood Center of Riberirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14051-140, SP, Brazil., Alcantara LCJ; Instituto Rene Rachou, Fundação Oswaldo Cruz, Belo Horizonte 30190-002, MG, Brazil.; Climate Amplified Diseases and Epidemic (CLIMADE), Brasilia 70070-130, DF, Brazil., Sampaio SC; Laboratory of Cell Cycle (LECC), Center for Scientific Development (CDC), Butantan Institute, São Paulo 05585-000, SP, Brazil., Giovanetti M; Instituto Rene Rachou, Fundação Oswaldo Cruz, Belo Horizonte 30190-002, MG, Brazil.; Climate Amplified Diseases and Epidemic (CLIMADE), Brasilia 70070-130, DF, Brazil.; Department of Science and Technology for Humans and the Environment, University of Campus Bio-Medico di Roma, 00128 Rome, Italy.; Laboratório de Arbovírus e Vírus Hemorrágicos, Instituto Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil., Elias MC; Laboratory of Cell Cycle (LECC), Center for Scientific Development (CDC), Butantan Institute, São Paulo 05585-000, SP, Brazil., Slavov SN; Laboratory of Cell Cycle (LECC), Center for Scientific Development (CDC), Butantan Institute, São Paulo 05585-000, SP, Brazil. |
المصدر: | Microorganisms [Microorganisms] 2024 Apr 11; Vol. 12 (4). Date of Electronic Publication: 2024 Apr 11. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101625893 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2607 (Print) Linking ISSN: 20762607 NLM ISO Abbreviation: Microorganisms Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI AG, [2013]- |
مستخلص: | Viral hemorrhagic fever poses a significant public health challenge due to its severe clinical presentation and high mortality rate. The diagnostic process is hindered by similarity of symptoms across different diseases and the broad spectrum of pathogens that can cause hemorrhagic fever. In this study, we applied viral metagenomic analysis to 43 serum samples collected by the Public Health Laboratory ( Fundação Ezequiel Dias , FUNED) in Minas Gerais State, Brazil, from patients diagnosed with hemorrhagic fever who had tested negative for the standard local hemorrhagic disease testing panel. This panel includes tests for Dengue virus (DENV) IgM, Zika virus IgM, Chikungunya virus IgM, yellow fever IgM, Hantavirus IgM, Rickettsia rickettsii IgM/IgG, and Leptospira interrogans IgM, in addition to respective molecular tests for these infectious agents. The samples were grouped into 18 pools according to geographic origin and analyzed through next-generation sequencing on the NextSeq 2000 platform. Bioinformatic analysis revealed a prevalent occurrence of commensal viruses across all pools, but, notably, a significant number of reads corresponding to the DENV serotype 2 were identified in one specific pool. Further verification via real-time PCR confirmed the presence of DENV-2 RNA in an index case involving an oncology patient with hemorrhagic fever who had initially tested negative for anti-DENV IgM antibodies, thereby excluding this sample from initial molecular testing. The complete DENV-2 genome isolated from this patient was taxonomically classified within the cosmopolitan genotype that was recently introduced into Brazil. These findings highlight the critical role of considering the patient's clinical condition when deciding upon the most appropriate testing procedures. Additionally, this study showcases the potential of viral metagenomics in pinpointing the viral agents behind hemorrhagic diseases. Future research is needed to assess the practicality of incorporating metagenomics into standard viral diagnostic protocols. |
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معلومات مُعتمدة: | 2017/23205-8; 2021/11944-6; 2022/00910-6; 2022/10278-5 Fundação de Amparo à Pesquisa do Estado de São Paulo |
فهرسة مساهمة: | Keywords: Brazil; hemorrhagic fever; next-generation sequencing; viral diagnosis; viral metagenomics |
تواريخ الأحداث: | Date Created: 20240427 Latest Revision: 20240429 |
رمز التحديث: | 20240429 |
مُعرف محوري في PubMed: | PMC11052305 |
DOI: | 10.3390/microorganisms12040769 |
PMID: | 38674713 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2076-2607 |
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DOI: | 10.3390/microorganisms12040769 |