دورية أكاديمية
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A near-infrared fluorescent probe based FRET for ratiometric sensing of H 2 O 2 and viscosity in live cells.

التفاصيل البيبلوغرافية
العنوان: A near-infrared fluorescent probe based FRET for ratiometric sensing of H 2 O 2 and viscosity in live cells.
المؤلفون: Liu FT; Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China., Wang S; Institute of Developmental Biology, School of Life Science, Shandong University, Qingdao 266237, PR China., Wang YP; Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China., Jiang PF; Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China., Miao JY; Institute of Developmental Biology, School of Life Science, Shandong University, Qingdao 266237, PR China., Zhao BX; Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China. Electronic address: bxzhao@sdu.edu.cn., Lin ZM; Institute of Medical Sciences, The Second Hospital of Shandong University, Jinan 250033, PR China. Electronic address: linzhaomin@sdu.edu.cn.
المصدر: Talanta [Talanta] 2024 Aug 01; Vol. 275, pp. 126135. Date of Electronic Publication: 2024 Apr 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
مواضيع طبية MeSH: Hydrogen Peroxide*/analysis , Hydrogen Peroxide*/chemistry , Fluorescent Dyes*/chemistry , Fluorescence Resonance Energy Transfer*/methods, Humans ; HeLa Cells ; Viscosity ; Infrared Rays ; Limit of Detection ; Cell Survival
مستخلص: Hydrogen peroxide (H 2 O 2 ) and viscosity play vital roles in the cellular environment as signaling molecule and microenvironment parameter, respectively, and are associated with many physiological and pathological processes in biological systems. We developed a near-infrared fluorescent probe, CQ, which performed colorimetric and ratiometric detection of H 2 O 2 and viscosity based on the FRET mechanism, and was capable of monitoring changes in viscosity and H 2 O 2 levels simultaneously through two different channels. Based on the specific reaction of H 2 O 2 with borate ester, CQ exhibited a significant ratiometric response to H 2 O 2 with a large Stokes shift of 221 nm, a detection limit of 0.87 μM, a near-infrared emission wavelength of 671 nm, a response time of 1 h, a wide detection ranges of 0.87-800 μM and a high energy transfer efficiency of 99.9 %. CQ could also recognize viscosity by the TICT mechanism, and efficiently detect viscosity changes caused by food thickeners. More importantly, CQ could successfully detect endogenous/exogenous H 2 O 2 and viscosity in live HeLa cells, which was expected to be a practical tool for detecting H 2 O 2 and viscosity in live cells.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Bio-imaging; FRET probe; Hydrogen peroxide; NIR; Viscosity
المشرفين على المادة: BBX060AN9V (Hydrogen Peroxide)
0 (Fluorescent Dyes)
تواريخ الأحداث: Date Created: 20240427 Date Completed: 20240529 Latest Revision: 20240529
رمز التحديث: 20240529
DOI: 10.1016/j.talanta.2024.126135
PMID: 38677165
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-3573
DOI:10.1016/j.talanta.2024.126135