Anti-NSCLC role of SCN4B by negative regulation of the cGMP-PKG pathway: Integrated utilization of bioinformatics analysis and in vitro assay validation.

التفاصيل البيبلوغرافية
العنوان:	Anti-NSCLC role of SCN4B by negative regulation of the cGMP-PKG pathway: Integrated utilization of bioinformatics analysis and in vitro assay validation.
المؤلفون:	Yang X; Department of Respiratory and Critical Care Medicine, Huai'an People's Hospital of Hongze District, Huai'an, China., Liu Q; Medical Ward 20, Lianshui County People's Hospital, Huai'an, China., Li G; Department of Respiratory and Critical Care Medicine, Huai'an Second People's Hospital, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China.
المصدر:	Drug development research [Drug Dev Res] 2024 May; Vol. 85 (3), pp. e22192.
نوع المنشور:	Journal Article; Research Support, Non-U.S. Gov't
اللغة:	English
بيانات الدورية:	Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8204468 Publication Model: Print Cited Medium: Internet ISSN: 1098-2299 (Electronic) Linking ISSN: 02724391 NLM ISO Abbreviation: Drug Dev Res Subsets: MEDLINE
أسماء مطبوعة:	Publication: New York Ny : Wiley-Liss Original Publication: New York : Alan R. Liss, c1981-
مواضيع طبية MeSH:	Carcinoma, Non-Small-Cell Lung/genetics , Carcinoma, Non-Small-Cell Lung/metabolism , Carcinoma, Non-Small-Cell Lung/pathology , Computational Biology , Cyclic GMP/metabolism , Lung Neoplasms/genetics , Lung Neoplasms/metabolism , Lung Neoplasms/pathology, Humans ; A549 Cells ; Apoptosis ; Cell Line, Tumor ; Cell Survival ; Cyclic GMP-Dependent Protein Kinases/metabolism ; Cyclic GMP-Dependent Protein Kinases/genetics ; Down-Regulation ; Gene Expression Regulation, Neoplastic ; Signal Transduction
مستخلص:	Non-small cell lung cancer (NSCLC) is a malignant tumor with low overall cure and survival rates. Uncovering abnormally expressed genes is significantly important for developing novel targeted therapies in NSCLC. This study aimed to discover new differentially expressed genes (DEGs) of NSCLC. The DEGs of NSCLC were identified in eight data sets from Gene Expression Omnibus (GEO) database. The expression profiles and the prognostic significance of SCN4B in LUAD and LUSC were analyzed using GEPIA database. LinkedOmics was used to identify co-expressed genes with SCN4B, which were further subjected to KEGG pathway enrichment analysis. SCN4B-overexpressing plasmid (pcDNA/SCN4B) was transfected into A549 and NCI-H2170 cells to elevate the expression of SCN4B. MTT and TUNEL assays were performed to evaluate cell viability and apoptosis. Relying on the screened DEGs from GEO database, we identified that SCN4B was significantly downregulated in LUAD and LUSC. We confirmed the downregulation of SCN4B in NSCLC tissues using GEPIA database. SCN4B has a prognostic value in LUAD, but not LUSC. KEGG pathway enrichment analysis of SCN4B-related genes showed that cGMP-PKG signaling pathway might be involved in the role of SCN4B in NSCLC. Overexpression of SCN4B in A549 and NCI-H2170 cells inhibited the cell viability. Besides, SCN4B overexpression induced apoptosis of A549 and NCI-H2170 cells. SCN4B inhibited the expression of PKG1 and p-CREB in NSCLC cells. Moreover, the inhibitory effects of SCN4B on tumor malignancy were attenuated by the activator of PKG. In conclusion, integrated bioinformatical analysis proved that SCN4B was downregulated and had a prognostic significance in NSCLC. In vitro experimental studies demonstrated that SCN4B regulated NSCLC cells viability and apoptosis via inhibiting cGMP-PKG signaling pathway. (© 2024 Wiley Periodicals LLC.)
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فهرسة مساهمة:	Keywords: SCN4B; bioinformatical analysis; cGMP‐PKG; differentially expressed genes; non‐small cell lung cancer
المشرفين على المادة:	H2D2X058MU (Cyclic GMP) EC 2.7.11.12 (Cyclic GMP-Dependent Protein Kinases) 0 (SCN4B protein, human)
تواريخ الأحداث:	Date Created: 20240428 Date Completed: 20240428 Latest Revision: 20240507
رمز التحديث:	20240508
DOI:	10.1002/ddr.22192
PMID:	38678552
قاعدة البيانات:	MEDLINE

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تدمد:	1098-2299
DOI:	10.1002/ddr.22192