دورية أكاديمية
أعرض في EDS

A 72-h sedated porcine model of traumatic spinal cord injury.

التفاصيل البيبلوغرافية
العنوان: A 72-h sedated porcine model of traumatic spinal cord injury.
المؤلفون: Thygesen MM; Department of Neurosurgery, Aarhus University Hospital, Denmark.; Department of Clinical Medicine CENSE, Aarhus University, Denmark.; Department of Clinical Medicine Comparative Medicine Lab, Aarhus University, Denmark., Entezari S; Department of Neurosurgery, Aarhus University Hospital, Denmark.; Department of Clinical Medicine CENSE, Aarhus University, Denmark.; Department of Clinical Medicine Comparative Medicine Lab, Aarhus University, Denmark., Houlind N; Department of Neurosurgery, Aarhus University Hospital, Denmark.; Department of Clinical Medicine CENSE, Aarhus University, Denmark.; Department of Clinical Medicine Comparative Medicine Lab, Aarhus University, Denmark., Nielsen TH; Department of Neurosurgery, Aarhus University Hospital, Denmark.; Department of Clinical Medicine CENSE, Aarhus University, Denmark.; Department of Clinical Medicine Comparative Medicine Lab, Aarhus University, Denmark., Olsen NØ; Department of Neurosurgery, Aarhus University Hospital, Denmark.; Department of Clinical Medicine CENSE, Aarhus University, Denmark.; Department of Clinical Medicine Comparative Medicine Lab, Aarhus University, Denmark., Nielsen TD; Department of Neurosurgery, Aarhus University Hospital, Denmark.; Department of Clinical Medicine CENSE, Aarhus University, Denmark.; Department of Clinical Medicine Comparative Medicine Lab, Aarhus University, Denmark., Skov M; Department of Clinical Medicine Comparative Medicine Lab, Aarhus University, Denmark., Borgstedt-Bendixen J; Department of Neurosurgery, Aarhus University Hospital, Denmark., Tankisi A; Department of Anesthesiology, Aarhus University Hospital, Denmark., Rasmussen M; Department of Anesthesiology, Aarhus University Hospital, Denmark., Einarsson HB; Department of Neurosurgery, Aarhus University Hospital, Denmark., Agger P; Department of Clinical Medicine Comparative Medicine Lab, Aarhus University, Denmark., Orlowski D; Department of Neurosurgery, Aarhus University Hospital, Denmark., Dyrskog SE; Department of Intensive Care, Aarhus University Hospital, Denmark., Thorup L; Department of Intensive Care, Aarhus University Hospital, Denmark., Pedersen M; Department of Clinical Medicine Comparative Medicine Lab, Aarhus University, Denmark., Rasmussen MM; Department of Neurosurgery, Aarhus University Hospital, Denmark.; Department of Clinical Medicine CENSE, Aarhus University, Denmark.
المصدر: Brain & spine [Brain Spine] 2024 Apr 16; Vol. 4, pp. 102813. Date of Electronic Publication: 2024 Apr 16 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 9918470888906676 Publication Model: eCollection Cited Medium: Internet ISSN: 2772-5294 (Electronic) Linking ISSN: 27725294 NLM ISO Abbreviation: Brain Spine Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier B.V., [2021]-
مستخلص: Introduction: There is an increasing focus on the prevention of secondary injuries following traumatic spinal cord injury (TSCI), especially through improvement of spinal cord perfusion and immunological modulation. Such therapeutic strategies require translational and controlled animal models of disease progression of the acute phases of human TSCI.
Research Question: Is it possible to establish a 72-h sedated porcine model of incomplete thoracic TSCI, enabling controlled use of continuous, invasive, and non-invasive modalities during the entire sub-acute phase of TSCI?
Material and Methods: A sham-controlled trial was conducted to establish the model, and 10 animals were assigned to either sham or TSCI. All animals underwent a laminectomy, and animals in the TSCI group were subjected to a weight-drop injury. Animals were then kept sedated for 72 h. The amount of injury was assessed by ex-vivo measures MRI-based fiber tractography, histology and immunohistochemistry.
Results: In all animals, we were successful in maintaining sedation for 72 h without comprising vital physiological parameters. The MRI-based fiber tractography showed that all TSCI animals revealed a break in the integrity of spinal neurons, whereas histology demonstrated no transversal sections of the spine with complete injury. Notably, some animals displayed signs of secondary ischemic tissue in the cranial and caudal sections.
Discussion and Conclusions: This study succeeded in producing a porcine model of incomplete TSCI, which was physiologically stable up to 72 h. We believe that this TSCI model will constitute a potential translational model to study the pathophysiology secondary to TSCI in humans.
Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Mathias Thygesen reports financial support was provided by Offerfonden (Victims Foundation, Ministry of Justice Denmark). Mathias Thygesen reports financial support was provided by The Riisfort Foundataion. Mathias Thygesen reports financial support was provided by AP Møller Mærsk Foundation. Mathias Thygesen reports financial support was provided by The Dagmar Marshalls Foundation.
(© 2024 The Authors.)
References: Radiographics. 2020 Mar-Apr;40(2):403-427. (PMID: 32125961)
Brain Inj. 2017;31(2):230-236. (PMID: 28055267)
Acta Neurochir Suppl. 2005;95:43-9. (PMID: 16463818)
Exp Neurol. 2015 Sep;271:131-5. (PMID: 26044197)
Neurotrauma Rep. 2022 Sep 01;3(1):352-368. (PMID: 36204385)
Global Spine J. 2016 Sep;6(6):607-14. (PMID: 27556002)
J Neurosurg. 1991 Jul;75(1):15-26. (PMID: 2045903)
Magn Reson Med. 2013 Jan;69(1):144-9. (PMID: 22396180)
J Physiol. 2020 Mar;598(5):929-942. (PMID: 31876952)
Neurology. 2019 Sep 17;93(12):e1205-e1211. (PMID: 31409736)
Dis Model Mech. 2014 Jun;7(6):601-11. (PMID: 24719025)
J Neurotrauma. 2013 Feb 1;30(3):142-59. (PMID: 23316955)
PLoS One. 2009;4(3):e4764. (PMID: 19274095)
Eur Spine J. 2021 Oct;30(10):3028-3035. (PMID: 34170417)
J Neurosci. 2009 Oct 28;29(43):13435-44. (PMID: 19864556)
Neuroimage. 2011 Apr 1;55(3):1024-33. (PMID: 21232610)
J Clin Orthop Trauma. 2022 Sep 11;34:102024. (PMID: 36147378)
Neurosurgery. 2015 Apr;76(4):461-8; discussion 468-9. (PMID: 25621979)
Neuroimage Clin. 2015 Dec 04;10:192-238. (PMID: 26862478)
J Neurosurg Sci. 1995 Mar;39(1):67-74. (PMID: 8568556)
J Neurotrauma. 2017 Nov 1;34(21):2964-2971. (PMID: 28385062)
Neurosurgery. 2002 Mar;50(3 Suppl):S166-72. (PMID: 12431301)
J Trauma. 1989 Oct;29(10):1440-7. (PMID: 2810423)
J Neurotrauma. 2021 Sep 15;38(18):2560-2571. (PMID: 33858218)
Lab Anim Sci. 1990 May;40(3):293-8. (PMID: 2162986)
Nat Protoc. 2021 Oct;16(10):4611-4632. (PMID: 34400839)
Ann N Y Acad Sci. 2010 Jun;1198 Suppl 1:E22-34. (PMID: 20590535)
AJNR Am J Neuroradiol. 2008 Apr;29(4):655-9. (PMID: 18238846)
J Neurotrauma. 2019 Mar 19;36(6):919-929. (PMID: 30351245)
J Neurotrauma. 2018 Jun 15;35(12):1345-1357. (PMID: 29338544)
Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772)
J Trauma. 1988 Apr;28(4):481-90. (PMID: 3352009)
Stroke. 1989 Mar;20(3):372-7. (PMID: 2922776)
Crit Care. 2016 Oct 5;20(1):308. (PMID: 27716379)
Top Spinal Cord Inj Rehabil. 2021 Spring;27(2):1-22. (PMID: 34108832)
J Neurotrauma. 2012 Sep;29(13):2318-27. (PMID: 21639753)
J Neurosurg Spine. 2009 Mar;10(3):181-93. (PMID: 19320576)
J Neurotrauma. 2021 Nov 1;38(21):2937-2955. (PMID: 34011164)
Neuroimage. 2019 Nov 15;202:116137. (PMID: 31473352)
Exp Neurol. 1999 Aug;158(2):351-65. (PMID: 10415142)
Neurosurgery. 2020 Sep 1;87(3):427-434. (PMID: 32761068)
Stroke. 1998 Sep;29(9):1783-90. (PMID: 9731595)
J Neurotrauma. 2017 Dec 15;34(24):3336-3350. (PMID: 28844181)
Sci Rep. 2020 Oct 1;10(1):16223. (PMID: 33004849)
Cold Spring Harb Perspect Biol. 2014 Dec 04;7(3):a020602. (PMID: 25475091)
Stroke. 1986 Nov-Dec;17(6):1183-9. (PMID: 3810718)
Neurosurgery. 2013 Mar;72 Suppl 2:84-92. (PMID: 23417181)
J Neurosurg. 1976 Jan;44(1):12-5. (PMID: 811767)
J Neurotrauma. 2015 Jun 15;32(12):865-74. (PMID: 25705999)
J Neurotrauma. 2021 Nov 1;38(21):2956-2977. (PMID: 34121450)
Neurotherapeutics. 2020 Apr;17(2):511-521. (PMID: 31916236)
Crit Care Med. 2014 Mar;42(3):646-55. (PMID: 24231762)
Exp Neurol. 2014 Aug;258:121-129. (PMID: 25017893)
J Neurotrauma. 2017 Feb;34(3):541-551. (PMID: 27492951)
فهرسة مساهمة: Keywords: Critical care model; Diffusion magnetic resonance imaging; Translational animal model; Traumatic spinal cord injury
تواريخ الأحداث: Date Created: 20240429 Latest Revision: 20240430
رمز التحديث: 20240430
مُعرف محوري في PubMed: PMC11052900
DOI: 10.1016/j.bas.2024.102813
PMID: 38681174
قاعدة البيانات: MEDLINE
الوصف
تدمد:2772-5294
DOI:10.1016/j.bas.2024.102813