دورية أكاديمية
أعرض في EDS

Arf GTPase-Activating proteins ADAP1 and ARAP1 regulate incorporation of CD63 in multivesicular bodies.

التفاصيل البيبلوغرافية
العنوان: Arf GTPase-Activating proteins ADAP1 and ARAP1 regulate incorporation of CD63 in multivesicular bodies.
المؤلفون: Suzuki K; Graduate course of Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate University, 020-8551, Morioka, Japan., Okawa Y; Graduate course of Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate University, 020-8551, Morioka, Japan., Akter S; Graduate course of Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate University, 020-8551, Morioka, Japan., Ito H; Biological Sciences Course, Faculty of Science and Engineering, Iwate University, 020-8551, Morioka, Japan., Shiba Y; Graduate course of Biological Sciences, Division of Science and Engineering, Graduate School of Arts and Sciences, Iwate University, 020-8551, Morioka, Japan.; Biological Sciences Course, Faculty of Science and Engineering, Iwate University, 020-8551, Morioka, Japan.
المصدر: Biology open [Biol Open] 2024 May 15; Vol. 13 (5). Date of Electronic Publication: 2024 May 10.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Company of Biologists Ltd Country of Publication: England NLM ID: 101578018 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2046-6390 (Electronic) Linking ISSN: 20466390 NLM ISO Abbreviation: Biol Open Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Cambridge : Company of Biologists Ltd.
مواضيع طبية MeSH: Adaptor Proteins, Signal Transducing*/metabolism , Adaptor Proteins, Signal Transducing*/genetics , GTPase-Activating Proteins*/metabolism , Multivesicular Bodies*/metabolism , Tetraspanin 30*/metabolism, Humans ; ADP-Ribosylation Factors/metabolism ; ADP-Ribosylation Factors/genetics ; Endosomes/metabolism ; HeLa Cells ; Protein Transport ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism
مستخلص: Arf GTPase-activating proteins (ArfGAPs) mediate the hydrolysis of GTP bound to ADP-ribosylation factors. ArfGAPs are critical for cargo sorting in the Golgi-to-ER traffic. However, the role of ArfGAPs in sorting into intralumenal vesicles (ILVs) in multivesicular bodies (MVBs) in post-Golgi traffic remains unclear. Exosomes are extracellular vesicles (EVs) of endosomal origin. CD63 is an EV marker. CD63 is enriched ILVs in MVBs of cells. However, the secretion of CD63 positive EVs has not been consistent with the data on CD63 localization in MVBs, and how CD63-containing EVs are formed is yet to be understood. To elucidate the mechanism of CD63 transport to ILVs, we focused on CD63 localization in MVBs and searched for the ArfGAPs involved in CD63 localization. We observed that ADAP1 and ARAP1 depletion inhibited CD63 localization to enlarged endosomes after Rab5Q79L overexpression. We tested epidermal growth factor (EGF) and CD9 localization in MVBs. We observed that ADAP1 and ARAP1 depletion inhibited CD9 localization in enlarged endosomes but not EGF. Our results indicate ADAP1 and ARAP1, regulate incorporation of CD63 and CD9, but not EGF, in overlapped and different MVBs. Our work will contribute to distinguish heterogenous ILVs and exosomes by ArfGAPs.
Competing Interests: Competing interests The authors declare no competing or financial interests.
(© 2024. Published by The Company of Biologists Ltd.)
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معلومات مُعتمدة: Iwate University; Iwate University: Iwate Daigaku
فهرسة مساهمة: Keywords: ArfGAP; CD63; Exosome; Extracellular vesicles; Intralumenal vesicles; Multivesicular bodies
المشرفين على المادة: 0 (Adaptor Proteins, Signal Transducing)
EC 3.6.5.2 (ADP-Ribosylation Factors)
0 (ARAP1 protein, human)
0 (CD63 protein, human)
0 (GTPase-Activating Proteins)
0 (Tetraspanin 30)
0 (ADAP1 protein, human)
0 (Nerve Tissue Proteins)
تواريخ الأحداث: Date Created: 20240429 Date Completed: 20240510 Latest Revision: 20240627
رمز التحديث: 20240627
مُعرف محوري في PubMed: PMC11103404
DOI: 10.1242/bio.060338
PMID: 38682696
قاعدة البيانات: MEDLINE
الوصف
تدمد:2046-6390
DOI:10.1242/bio.060338