دورية أكاديمية
أعرض في EDS

Insecticidal activity of the spider neurotoxin PPTX-04 through modulating insect voltage-gated sodium channel.

التفاصيل البيبلوغرافية
العنوان: Insecticidal activity of the spider neurotoxin PPTX-04 through modulating insect voltage-gated sodium channel.
المؤلفون: Wang K; Key laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China., Yan Y; Key laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China., Huang L; Department of Applied Microbiology, Jiangsu Lixiahe District Institute of Agricultural Sciences/National Agricultural Experimental Station for Agricultural Microbiology, Yangzhou 225007, China., Sun H; State Key Laboratory of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin 30071, China., Yu N; Key laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China., Liu Z; Key laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: liuzewen@njau.edu.cn.
المصدر: Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2024 May; Vol. 201, pp. 105853. Date of Electronic Publication: 2024 Mar 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 1301573 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9939 (Electronic) Linking ISSN: 00483575 NLM ISO Abbreviation: Pestic Biochem Physiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York Ny : Academic Press
مواضيع طبية MeSH: Insecticides*/pharmacology , Insecticides*/chemistry , Spider Venoms*/chemistry , Spider Venoms*/pharmacology , Spider Venoms*/genetics , Voltage-Gated Sodium Channels*/metabolism , Voltage-Gated Sodium Channels*/genetics , Spiders* , Neurotoxins*/pharmacology , Neurotoxins*/toxicity, Animals ; Pyrethrins/pharmacology ; Hemiptera/drug effects ; Oocytes/drug effects ; Xenopus laevis ; Insect Proteins/genetics ; Insect Proteins/metabolism ; Insect Proteins/chemistry
مستخلص: Ion channels on cell membrane are molecular targets of more than half peptide neurotoxins from spiders. From Pardosa pseudoannulata, a predatory spider on a range of insect pests, we characterized a peptide neurotoxin PPTX-04 with an insecticidal activity. PPTX-04 showed high toxicity to Nilaparvata lugens, a main prey of P. pseudoannulata, and the toxicity was not affected by the resistance to etofenprox (IUPAC chemical name:1-ethoxy-4-[2-methyl-1-[(3-phenoxyphenyl)methoxy]propan-2-yl]benzene, purity: 99%). On N. lugens voltage-gated sodium channel NlNa v 1 expressed in Xenopus oocytes, PPTX-04 prolonged the channel opening and induced tail currents, which is similar to pyrethroid insecticides. However, PPTX-04 potency on NlNa v 1 was not affected by mutations conferring pyrethroid resistance in insects, which revealed that PPTX-04 and pyrethroids should act on different receptors in NlNa v 1. In contrast, two mutations at the extracellular site 4 significantly reduced PPTX-04 potency, which indicated that PPTX-04 would act on a potential receptor containing the site 4 in NlNa v 1. The result from the molecular docking supported the conclusion that the binding pocket of PPTX-04 in NlNa v 1 should contain the site 4. In summary, PPTX-04 had high insecticidal activity through acting on a distinct receptor site in insect Na v , and was a potential resource to control insect pests and manage resistance to pyrethroids.
Competing Interests: Declaration of competing interest None.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: PPTX-04; Pyrethroid; Resistance; Spider neurotoxin; Voltage-gated sodium channel Na(v)
المشرفين على المادة: 0 (Insecticides)
0 (Spider Venoms)
0 (Voltage-Gated Sodium Channels)
0 (Neurotoxins)
0 (Pyrethrins)
0 (Insect Proteins)
تواريخ الأحداث: Date Created: 20240430 Date Completed: 20240430 Latest Revision: 20240430
رمز التحديث: 20240501
DOI: 10.1016/j.pestbp.2024.105853
PMID: 38685212
قاعدة البيانات: MEDLINE
الوصف
تدمد:1095-9939
DOI:10.1016/j.pestbp.2024.105853