دورية أكاديمية
أعرض في EDS

Development and evaluation of test methods for the detection and enumeration of opportunistic waterborne pathogens from the hospital environment.

التفاصيل البيبلوغرافية
العنوان: Development and evaluation of test methods for the detection and enumeration of opportunistic waterborne pathogens from the hospital environment.
المؤلفون: Inkster T; Antimicrobial Resistance and Healthcare Associated Infection, Glasgow, UK. Electronic address: Teresa.Inkster2@nhs.scot., Caldwell I; Department of Microbiology, Glasgow Royal Infirmary, Glasgow, UK., Aird H; Food Water and Environmental Microbiology Laboratory York, UK Health Security Agency, York, UK., Willis C; Food Water and Environmental Microbiology Laboratory Porton, UK Health Security Agency, Salisbury, UK., Lai S; Food Water and Environmental Microbiology Services, Colindale, UK Health Security Agency London, UK., Mallon J; Department of Microbiology, Glasgow Royal Infirmary, Glasgow, UK.
المصدر: The Journal of hospital infection [J Hosp Infect] 2024 Jul; Vol. 149, pp. 98-103. Date of Electronic Publication: 2024 Apr 27.
نوع المنشور: Journal Article; Evaluation Study
اللغة: English
بيانات الدورية: Publisher: W.B. Saunders For The Hospital Infection Society Country of Publication: England NLM ID: 8007166 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2939 (Electronic) Linking ISSN: 01956701 NLM ISO Abbreviation: J Hosp Infect Subsets: MEDLINE
أسماء مطبوعة: Publication: London : W.B. Saunders For The Hospital Infection Society
Original Publication: New York, Academic Press.
مواضيع طبية MeSH: Hospitals* , Water Microbiology*, Humans ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Bacteriological Techniques/methods ; Bacteriological Techniques/standards ; Culture Media ; Gram-Negative Bacteria/isolation & purification ; Gram-Negative Bacteria/classification ; Temperature ; United Kingdom ; Bacterial Load/methods
مستخلص: Background: Many Gram-negative bacteria other than Pseudomonas aeruginosa have been implicated in waterborne outbreaks, but standardized laboratory detection methods for these organisms have not been established.
Aim: This study aimed to establish laboratory testing methodologies for six waterborne pathogens: Acinetobacter spp., Burkholderia spp., Cupriavidus spp., Delftia acidovorans, Elizabethkingia spp. and Stenotrophomonas maltophilia.
Methods: Water samples were spiked by UK Health Security Agency laboratories and sent to the Glasgow Royal Infirmary laboratory for analysis. Water samples were spiked with either a pure culture of target organism or the target organism in water containing normal background flora, to ensure that the methodology could identify organisms from a mixed culture. Volumes of 100 mL were filtered under negative pressure on to culture media and incubated at 30 °C and 37 °C. The incubation time was 7 days, with plates read on days 2, 5 and 7. Further identification of colonies was undertaken using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS).
Findings: Optimal recovery of organisms was obtained by culturing water samples on tryptic soy agar, chocolate bacitracin agar and pseudomonas selective agar. The optimal temperature for isolation was 30 °C. The optimal incubation time was 5 days, and MALDI-TOF MS identified all test species reliably.
Conclusion: The methodology described was able to detect the six tested waterborne pathogens reliably, and can be utilized by laboratories involved in testing water samples during outbreak investigations.
(Crown Copyright © 2024. Published by Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Gram-negative identification; Water testing; Water testing methodology
المشرفين على المادة: 0 (Culture Media)
تواريخ الأحداث: Date Created: 20240430 Date Completed: 20240628 Latest Revision: 20240628
رمز التحديث: 20240629
DOI: 10.1016/j.jhin.2024.04.008
PMID: 38685413
قاعدة البيانات: MEDLINE
الوصف
تدمد:1532-2939
DOI:10.1016/j.jhin.2024.04.008