دورية أكاديمية
Polysorbate 80 coated chitosan nanoparticles for delivery of α-melanocyte stimulating hormone analog (NDP-MSH) to the brain reverse cognitive impairment related to neuroinflammation produced by a high-fat diet (HFD).
| العنوان: | Polysorbate 80 coated chitosan nanoparticles for delivery of α-melanocyte stimulating hormone analog (NDP-MSH) to the brain reverse cognitive impairment related to neuroinflammation produced by a high-fat diet (HFD). |
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| المؤلفون: | Herrera G; Instituto de Farmacología Experimental de Córdoba, IFEC-CONICET. Departamento de Farmacología Otto Orshinger, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina., Scimonelli T; Instituto de Farmacología Experimental de Córdoba, IFEC-CONICET. Departamento de Farmacología Otto Orshinger, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina., Lasaga M; Instituto de Investigaciones Biomédicas INBIOMED UBA-CONICET, Facultad de Medicina, Universidad de Buenos Aires, Argentina., Granero G; Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA), UNC-CONICET, Departamento de Ciencias Farmacéuticas, Universidad Nacional de Córdoba, Córdoba, Argentina., Onnainty R; Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA), UNC-CONICET, Departamento de Ciencias Farmacéuticas, Universidad Nacional de Córdoba, Córdoba, Argentina. Electronic address: ronnainty@unc.edu.ar. |
| المصدر: | Neuropharmacology [Neuropharmacology] 2024 Aug 01; Vol. 253, pp. 109969. Date of Electronic Publication: 2024 Apr 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 0236217 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7064 (Electronic) Linking ISSN: 00283908 NLM ISO Abbreviation: Neuropharmacology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Pergamon Press Original Publication: Oxford, New York, Pergamon. |
| مواضيع طبية MeSH: | Chitosan*/administration & dosage , Chitosan*/chemistry , alpha-MSH*/administration & dosage , alpha-MSH*/analogs & derivatives , Rats, Wistar* , Polysorbates*/chemistry , Polysorbates*/administration & dosage , Cognitive Dysfunction*/metabolism , Cognitive Dysfunction*/drug therapy , Nanoparticles*/administration & dosage , Diet, High-Fat*/adverse effects , Brain*/metabolism , Brain*/drug effects, Animals ; Male ; Neuroinflammatory Diseases/metabolism ; Neuroinflammatory Diseases/drug therapy ; Rats |
| مستخلص: | This study aimed to develop polysorbate 80-coated chitosan nanoparticles (PS80/CS NPs) as a delivery system for improved brain targeting of α-Melanocyte Stimulating Hormone analog (NDP-MSH). Chitosan nanoparticles loaded with NDP-MSH were surface-modified with polysorbate 80 ([NDP-MSH]-PS80/CS NP), which formed a flattened layer on their surface. Nanoparticle preparation involved ionic gelation, followed by characterization using scanning electron microscopy (SEM) for morphology, dynamic light scattering (DLS) for colloidal properties, and ATR-FTIR spectroscopy for structure. Intraperitoneal injection of FITC-PS80/CS NPs and [NDP-MSH]-PS80/CS NP in rats demonstrated their ability to cross the blood-brain barrier, reach the brain, and accumulate in CA1 neurons of the dorsal hippocampus within 2 h. Two experimental models of neuroinflammation were employed with Male Wistar rats: a short-term model involving high-fat diet (HFD) consumption for 5 days followed by an immune stimulus with LPS, and a long-term model involving HFD consumption for 8 weeks. In both models, [NDP-MSH]-PS80/CS NPs could reverse the decreased expression of contextual fear memory induced by the diets. These findings suggest that [NDP-MSH]-PS80/CS NPs offer a promising strategy to overcome the limitations of NDP-MSH regarding pharmacokinetics and enzymatic stability. By facilitating NDP-MSH delivery to the hippocampus, these nanoparticles can potentially mitigate the cognitive impairments associated with HFD consumption and neuroinflammation. Competing Interests: Declaration of competing interest The authors declare no competing financial interest. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Brain delivery; Memory impairment; Polysorbate 80 coated chitosan nanoparticles; α-Melanocyte stimulating hormone analog (NDP-MSH) |
| المشرفين على المادة: | 0 (MSH, 4-Nle-7-Phe-alpha-) |
| تواريخ الأحداث: | Date Created: 20240430 Date Completed: 20240518 Latest Revision: 20240524 |
| رمز التحديث: | 20240524 |
| DOI: | 10.1016/j.neuropharm.2024.109969 |
| PMID: | 38688422 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-7064 |
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| DOI: | 10.1016/j.neuropharm.2024.109969 |