دورية أكاديمية
Dectin-1/SYK Activation Induces Antimicrobial Peptide and Negative Regulator of NF-κB Signaling in Human Oral Epithelial Cells.
| العنوان: | Dectin-1/SYK Activation Induces Antimicrobial Peptide and Negative Regulator of NF-κB Signaling in Human Oral Epithelial Cells. |
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| المؤلفون: | Inomata M; Division of Microbiology and Immunology, Department of Oral Biology and Tissue Engineering, Meikai University School of Dentistry, Sakado, Japan; inomata@dent.meikai.ac.jp., Abe M; Division of Microbiology and Immunology, Department of Oral Biology and Tissue Engineering, Meikai University School of Dentistry, Sakado, Japan., Kawase Y; Division of Microbiology and Immunology, Department of Oral Biology and Tissue Engineering, Meikai University School of Dentistry, Sakado, Japan., Hayashi T; Department of Anatomy Science, School of Allied Health Sciences, Kitasato University, Kitasato, Japan., Amano S; Research Institute of Odontology (M-RIO), Meikai University School of Dentistry, Sakado, Japan., Sakagami H; Research Institute of Odontology (M-RIO), Meikai University School of Dentistry, Sakado, Japan. |
| المصدر: | In vivo (Athens, Greece) [In Vivo] 2024 May-Jun; Vol. 38 (3), pp. 1042-1048. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: International Institute of Anticancer Research Country of Publication: Greece NLM ID: 8806809 Publication Model: Print Cited Medium: Internet ISSN: 1791-7549 (Electronic) Linking ISSN: 0258851X NLM ISO Abbreviation: In Vivo Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : Attiki, Greece : International Institute of Anticancer Research Original Publication: Athens : Dr. J.G. Delinassios |
| مواضيع طبية MeSH: | Lectins, C-Type*/metabolism , Lectins, C-Type*/genetics , NF-kappa B*/metabolism , Signal Transduction* , Syk Kinase*/metabolism , Syk Kinase*/genetics , Epithelial Cells*/metabolism , Epithelial Cells*/drug effects, Humans ; Cell Line ; Zymosan/pharmacology ; Cytokines/metabolism ; Cytokines/genetics ; Phosphorylation ; Mouth Mucosa/metabolism ; Mouth Mucosa/immunology ; Pore Forming Cytotoxic Proteins/metabolism ; Pore Forming Cytotoxic Proteins/genetics ; Antimicrobial Cationic Peptides/genetics ; Antimicrobial Cationic Peptides/metabolism |
| مستخلص: | Background/aim: Oral epithelial cells serve as the primary defense against microbial exposure in the oral cavity, including the fungus Candida albicans. Dectin-1 is crucial for recognition of β-glucan in fungi. However, expression and function of Dectin-1 in oral epithelial cells remain unclear. Materials and Methods: We assessed Dectin-1 expression in Ca9-22 (gingiva), HSC-2 (mouth), HSC-3 (tongue), and HSC-4 (tongue) human oral epithelial cells using flow cytometry and real-time polymerase chain reaction. Cell treated with β-glucan-rich zymosan were evaluated using real-time polymerase chain reaction. Phosphorylation of spleen-associated tyrosine kinase (SYK) was analyzed by western blotting. Results: Dectin-1 was expressed in all four cell types, with high expression in Ca9-22 and HSC-2. In Ca9-22 cells, exposure to β-glucan-rich zymosan did not alter the mRNA expression of chemokines nor of interleukin (IL)6, IL8, IL1β, IL17A, and IL17F. Zymosan induced the expression of antimicrobial peptides β-defensin-1 and LL-37, but not S100 calcium-binding protein A8 (S100A8) and S100A9. Furthermore, the expression of cylindromatosis (CYLD), a negative regulator of nuclear factor kappa B (NF-κB) signaling, was induced. In HSC-2 cells, zymosan induced the expression of IL17A. The expression of tumor necrosis factor alpha-induced protein 3 (TNFAIP3), a negative regulator of NF-κB signaling, was also induced. Expression of other cytokines and antimicrobial peptides remained unchanged. Zymosan induced phosphorylation of SYK in Ca9-22 cells, as well as NF-κB. Conclusion: Oral epithelial cells express Dectin-1 and recognize β-glucan, which activates SYK and induces the expression of antimicrobial peptides and negative regulators of NF-κB, potentially maintaining oral homeostasis. (Copyright © 2024, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.) |
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| فهرسة مساهمة: | Keywords: A20; CYLD; Candida albicans; Dectin-1; Human oral epithelial cells; LL-37; β-defensin 1 |
| المشرفين على المادة: | 0 (Lectins, C-Type) 0 (NF-kappa B) EC 2.7.10.2 (Syk Kinase) 0 (dectin 1) 9010-72-4 (Zymosan) 0 (Cytokines) 0 (Pore Forming Cytotoxic Proteins) 0 (Antimicrobial Cationic Peptides) |
| تواريخ الأحداث: | Date Created: 20240430 Date Completed: 20240430 Latest Revision: 20240504 |
| رمز التحديث: | 20240504 |
| مُعرف محوري في PubMed: | PMC11059888 |
| DOI: | 10.21873/invivo.13537 |
| PMID: | 38688646 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1791-7549 |
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| DOI: | 10.21873/invivo.13537 |