دورية أكاديمية
أعرض في EDS

Cold-shock proteome of myoblasts reveals role of RBM3 in promotion of mitochondrial metabolism and myoblast differentiation.

التفاصيل البيبلوغرافية
العنوان: Cold-shock proteome of myoblasts reveals role of RBM3 in promotion of mitochondrial metabolism and myoblast differentiation.
المؤلفون: Dey P; Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK-Post, Bellary Rd, Bengaluru, 560065, Karnataka, India.; SASTRA Deemed University, Tirumalaisamudram, Thanjavur, 613401, Tamil Nadu, India., Rajalaxmi S; Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK-Post, Bellary Rd, Bengaluru, 560065, Karnataka, India., Saha P; Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK-Post, Bellary Rd, Bengaluru, 560065, Karnataka, India., Thakur PS; Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK-Post, Bellary Rd, Bengaluru, 560065, Karnataka, India., Hashmi MA; Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK-Post, Bellary Rd, Bengaluru, 560065, Karnataka, India.; Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India., Lal H; Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK-Post, Bellary Rd, Bengaluru, 560065, Karnataka, India.; Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India., Saini N; Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK-Post, Bellary Rd, Bengaluru, 560065, Karnataka, India., Singh N; Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK-Post, Bellary Rd, Bengaluru, 560065, Karnataka, India., Ramanathan A; Institute for Stem Cell Science and Regenerative Medicine (inStem), GKVK-Post, Bellary Rd, Bengaluru, 560065, Karnataka, India. arvind@instem.res.in.
المصدر: Communications biology [Commun Biol] 2024 Apr 30; Vol. 7 (1), pp. 515. Date of Electronic Publication: 2024 Apr 30.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN: 2399-3642 (Electronic) Linking ISSN: 23993642 NLM ISO Abbreviation: Commun Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London, United Kingdom : Nature Publishing Group UK, [2018]-
مواضيع طبية MeSH: RNA-Binding Proteins*/metabolism , RNA-Binding Proteins*/genetics , Cell Differentiation* , Myoblasts*/metabolism , Mitochondria*/metabolism , Proteome*/metabolism, Animals ; Mice ; Cold-Shock Response ; Cell Line
مستخلص: Adaptation to hypothermia is important for skeletal muscle cells under physiological stress and is used for therapeutic hypothermia (mild hypothermia at 32  °C). We show that hypothermic preconditioning at 32  °C for 72 hours improves the differentiation of skeletal muscle myoblasts using both C2C12 and primary myoblasts isolated from 3 month and 18-month-old mice. We analyzed the cold-shock proteome of myoblasts exposed to hypothermia (32  °C for 6 and 48 h) and identified significant changes in pathways related to RNA processing and central carbon, fatty acid, and redox metabolism. The analysis revealed that levels of the cold-shock protein RBM3, an RNA-binding protein, increases with both acute and chronic exposure to hypothermic stress, and is necessary for the enhanced differentiation and maintenance of mitochondrial metabolism. We also show that overexpression of RBM3 at 37  °C is sufficient to promote mitochondrial metabolism, cellular proliferation, and differentiation of C2C12 and primary myoblasts. Proteomic analysis of C2C12 myoblasts overexpressing RBM3 show significant enrichment of pathways involved in fatty acid metabolism, RNA metabolism and the electron transport chain. Overall, we show that the cold-shock protein RBM3 is a critical factor that can be used for controlling the metabolic network of myoblasts.
(© 2024. The Author(s).)
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معلومات مُعتمدة: NA Department of Biotechnology, Ministry of Science and Technology (DBT)
المشرفين على المادة: 0 (RNA-Binding Proteins)
0 (Proteome)
0 (Rbm3 protein, mouse)
تواريخ الأحداث: Date Created: 20240430 Date Completed: 20240430 Latest Revision: 20240503
رمز التحديث: 20240503
مُعرف محوري في PubMed: PMC11061143
DOI: 10.1038/s42003-024-06196-4
PMID: 38688991
قاعدة البيانات: MEDLINE
الوصف
تدمد:2399-3642
DOI:10.1038/s42003-024-06196-4