دورية أكاديمية
Calcium modulation of bacterial wilt disease on potato.
| العنوان: | Calcium modulation of bacterial wilt disease on potato. |
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| المؤلفون: | Ferreira MV; Área Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay., Naranjo E; Department of Entomology and Plant Pathology, Auburn University, Auburn, Alabama, USA., Denis N; Área Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay., Cobine P; Department of Biological Sciences, Auburn University, Auburn, Alabama, USA., De La Fuente L; Department of Entomology and Plant Pathology, Auburn University, Auburn, Alabama, USA., Siri MI; Área Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay. |
| المصدر: | Applied and environmental microbiology [Appl Environ Microbiol] 2024 May 21; Vol. 90 (5), pp. e0024224. Date of Electronic Publication: 2024 May 01. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Solanum tuberosum*/microbiology , Plant Diseases*/microbiology , Calcium*/metabolism , Ralstonia solanacearum*/physiology , Ralstonia solanacearum*/genetics , Ralstonia solanacearum*/pathogenicity , Ralstonia solanacearum*/growth & development, Virulence ; Biofilms/growth & development ; Ralstonia/genetics ; Ralstonia/physiology ; Plant Roots/microbiology ; Xylem/microbiology |
| مستخلص: | Ralstonia solanacearum species complex (RSSC) is a phytopathogenic bacterial group that causes bacterial wilt in several crops, being potato ( Solanum tuberosum ) one of the most important hosts. The relationship between the potato plant ionome (mineral and trace elements composition) and the resistance levels to this pathogen has not been addressed until now. Mineral content of xylem sap, roots, stems and leaves of potato genotypes with different levels of resistance to bacterial wilt was assessed in this work, revealing a positive correlation between divalent calcium (Ca) cation concentrations and genotype resistance. The aim of this study was to investigate the effect of Ca on bacterial wilt resistance, and on the growth and virulence of RSSC. Ca supplementation significantly decreased the growth rate of Ralstonia pseudosolanacearum GMI1000 in minimal medium and affected several virulence traits such as biofilm formation and twitching motility. We also incorporate for the first time the use of microfluidic chambers to follow the pathogen growth and biofilm formation in conditions mimicking the plant vascular system. By using this approach, a reduction in biofilm formation was observed when both, rich and minimal media, were supplemented with Ca. Assessment of the effect of Ca amendments on bacterial wilt progress in potato genotypes revealed a significant delay in disease progress, or a complete absence of wilting symptoms in the case of partially resistant genotypes. This work contributes to the understanding of Ca effect on virulence of this important pathogen and provides new strategies for an integrated control of bacterial wilt on potato. Importance: Ralstonia solanacearum species complex (RSSC) includes a diverse group of bacterial strains that cause bacterial wilt. This disease is difficult to control due to pathogen aggressiveness, persistence, wide range of hosts, and wide geographic distribution in tropical, subtropical, and temperate regions. RSSC causes considerable losses depending on the pathogen strain, host, soil type, environmental conditions, and cultural practices. In potato, losses of $19 billion per year have been estimated for this pathogen worldwide. In this study, we report for the first time the mineral composition found in xylem sap and plant tissues of potato germplasm with different levels of resistance to bacterial wilt. This study underscores the crucial role of calcium (Ca) concentration in the xylem sap and stem in relation to the resistance of different genotypes. Our in vitro experiments provide evidence of Ca's inhibitory effect on the growth, biofilm formation, and twitching movement of the model RSSC strain R . pseudosolanacearum GMI1000. This study introduces a novel element, the Ca concentration, which should be included into the integrated disease control management strategies for bacterial wilt in potatoes. Competing Interests: The authors declare no conflict of interest. |
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| معلومات مُعتمدة: | N/A Programa de Desarrollo de las Ciencias Básicas (PEDECIBA); PhD Scholarship Academic Postgraduate Committee (CAP); N/A AU | Alabama Agricultural Experiment Station (AAES) |
| فهرسة مساهمة: | Keywords: bacterial wilt; calcium; disease resistance; plant ionome |
| SCR Organism: | Ralstonia pseudosolanacearum |
| تواريخ الأحداث: | Date Created: 20240501 Date Completed: 20240521 Latest Revision: 20240523 |
| رمز التحديث: | 20240523 |
| مُعرف محوري في PubMed: | PMC11107177 |
| DOI: | 10.1128/aem.00242-24 |
| PMID: | 38690890 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5336 |
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| DOI: | 10.1128/aem.00242-24 |