دورية أكاديمية
أعرض في EDS

Circ_0007445 inhibits trophoblast cell proliferation, migration and invasion by mediating the miR-4432/HTRA1 axis in preeclampsia.

التفاصيل البيبلوغرافية
العنوان: Circ_0007445 inhibits trophoblast cell proliferation, migration and invasion by mediating the miR-4432/HTRA1 axis in preeclampsia.
المؤلفون: Shao W; Department of Gynecology, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou City, China., Cui J, Wang W
المصدر: Journal of hypertension [J Hypertens] 2024 Jul 01; Vol. 42 (7), pp. 1154-1162. Date of Electronic Publication: 2024 Feb 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wolters Kluwer Health, Inc Country of Publication: Netherlands NLM ID: 8306882 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1473-5598 (Electronic) Linking ISSN: 02636352 NLM ISO Abbreviation: J Hypertens Subsets: MEDLINE
أسماء مطبوعة: Publication: [Alphen aan den Rijn, the Netherlands] : Wolters Kluwer Health, Inc.
Original Publication: London ; New York : Gower Academic Pub., [1983-
مواضيع طبية MeSH: Pre-Eclampsia*/genetics , Pre-Eclampsia*/metabolism , MicroRNAs*/genetics , MicroRNAs*/metabolism , Trophoblasts*/metabolism , High-Temperature Requirement A Serine Peptidase 1*/genetics , High-Temperature Requirement A Serine Peptidase 1*/metabolism , RNA, Circular*/genetics , RNA, Circular*/metabolism , Cell Proliferation* , Cell Movement*, Humans ; Female ; Pregnancy ; Adult
مستخلص: Background: : Circular RNAs (circRNAs) have been shown to be extensively involved in preeclampsia progression. At present, the role of circ_0007445 in preeclampsia progression is not clear.
Methods: A total of 30 preeclampsia patients and 30 normal pregnant women were recruited in our study. The function of trophoblast cells was explored to clarify the role and mechanism of circ_0007445 on the preeclampsia progression. The expression of circ_0007445, microRNA (miR)-4432 and high temperature requirement A1 (HTRA1) was analyzed by quantitative real-time PCR. The proliferation, migration and invasion of trophoblast cells were determined by cell counting kit 8 assay, EdU assay, colony formation assay, flow cytometry, and transwell assay. Protein expression was examined by western blot analysis. Dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay and RNA pull-down assay were used to assess RNA interaction relationships.
Results: Our data suggested that circ_0007445 had increased expression in preeclampsia patients. Knockdown of circ_0007445 enhanced trophoblast cell proliferation, migration and invasion. MiR-4432 was lowly expressed in preeclampsia patients, and it could be sponged by circ_0007445. MiR-4432 inhibitor overturned the promotion effects of circ_0007445 knockdown on trophoblast cell functions. HTRA1 was highly expressed in preeclampsia patients, and it could be targeted by miR-4432. HTRA1 overexpression could also reverse the proliferation, migration and invasion of trophoblast cells promoted by miR-4432 mimic. In addition, circ_0007445 positively regulated HTRA1 through targeting miR-4432.
Conclusion: :Our results suggested that circ_0007445 facilitated the development of preeclampsia by suppressing trophoblast cell function through miR-4432/HTRA1 axis.
(Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.)
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المشرفين على المادة: 0 (MicroRNAs)
EC 3.4.21.- (High-Temperature Requirement A Serine Peptidase 1)
0 (RNA, Circular)
EC 3.4.21.- (HTRA1 protein, human)
0 (MIRN432 microRNA, human)
تواريخ الأحداث: Date Created: 20240501 Date Completed: 20240531 Latest Revision: 20240624
رمز التحديث: 20240624
DOI: 10.1097/HJH.0000000000003692
PMID: 38690926
قاعدة البيانات: MEDLINE
الوصف
تدمد:1473-5598
DOI:10.1097/HJH.0000000000003692