دورية أكاديمية
Simultaneous production of biosurfactant and extracellular unspecific peroxygenases by Moesziomyces aphidis XM01 enables an efficient strategy for crude oil degradation.
| العنوان: | Simultaneous production of biosurfactant and extracellular unspecific peroxygenases by Moesziomyces aphidis XM01 enables an efficient strategy for crude oil degradation. |
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| المؤلفون: | Song JZ; MOE Key Laboratory of Evolution and Marine Biodiversity, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China., Wang CQ; MOE Key Laboratory of Evolution and Marine Biodiversity, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China., Yu GS; MOE Key Laboratory of Evolution and Marine Biodiversity, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China., Sun Z; MOE Key Laboratory of Evolution and Marine Biodiversity, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China., Wu AH; MOE Key Laboratory of Evolution and Marine Biodiversity, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China., Chi ZM; MOE Key Laboratory of Evolution and Marine Biodiversity, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao 266003, China., Liu GL; MOE Key Laboratory of Evolution and Marine Biodiversity, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao 266003, China. Electronic address: liugl@ouc.edu.cn. |
| المصدر: | Journal of hazardous materials [J Hazard Mater] 2024 Jun 05; Vol. 471, pp. 134437. Date of Electronic Publication: 2024 Apr 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9422688 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3336 (Electronic) Linking ISSN: 03043894 NLM ISO Abbreviation: J Hazard Mater Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier, |
| مواضيع طبية MeSH: | Petroleum*/metabolism , Biodegradation, Environmental* , Surface-Active Agents*/metabolism , Surface-Active Agents*/chemistry , Glycolipids*/metabolism , Mixed Function Oxygenases*/metabolism , Mixed Function Oxygenases*/genetics, Polycyclic Aromatic Hydrocarbons/metabolism ; Polycyclic Aromatic Hydrocarbons/chemistry ; Alkanes/metabolism |
| مستخلص: | Crude oil is a hazardous pollutant that poses significant and lasting harm to human health and ecosystems. In this study, Moesziomyces aphidis XM01, a biosurfactant mannosylerythritol lipids (MELs)-producing yeast, was utilized for crude oil degradation. Unlike most microorganisms relying on cytochrome P450, XM01 employed two extracellular unspecific peroxygenases, MaUPO.1 and MaUPO.2, with preference for polycyclic aromatic hydrocarbons (PAHs) and n-alkanes respectively, thus facilitating efficient crude oil degradation. The MELs produced by XM01 exhibited a significant emulsification activity of 65.9% for crude oil and were consequently supplemented in an "exogenous MELs addition" strategy to boost crude oil degradation, resulting in an optimal degradation ratio of 72.3%. Furthermore, a new and simple "pre-MELs production" strategy was implemented, achieving a maximum degradation ratio of 95.9%. During this process, the synergistic up-regulation of MaUPO.1, MaUPO.1 and the key MELs synthesis genes contributed to the efficient degradation of crude oil. Additionally, the phylogenetic and geographic distribution analysis of MaUPO.1 and MaUPO.1 revealed their wide occurrence among fungi in Basidiomycota and Ascomycota, with high transcription levels across global ocean, highlighting their important role in biodegradation of crude oil. In conclusion, M. aphidis XM01 emerges as a novel yeast for efficient and eco-friendly crude oil degradation. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bioremediation; Biosurfactant; Crude oil degrading yeast; Degradation strategy; Unspecific peroxygenases |
| المشرفين على المادة: | 0 (Petroleum) 0 (Surface-Active Agents) 0 (Glycolipids) EC 1.- (Mixed Function Oxygenases) EC 1.14.- (peroxygenase) 0 (mannosylerythritol lipid) 0 (Polycyclic Aromatic Hydrocarbons) 0 (Alkanes) |
| تواريخ الأحداث: | Date Created: 20240501 Date Completed: 20240511 Latest Revision: 20240511 |
| رمز التحديث: | 20240511 |
| DOI: | 10.1016/j.jhazmat.2024.134437 |
| PMID: | 38691934 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3336 |
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| DOI: | 10.1016/j.jhazmat.2024.134437 |