دورية أكاديمية
The methylome of motile cilia.
العنوان: | The methylome of motile cilia. |
---|---|
المؤلفون: | King SM; Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT 3305., Sakato-Antoku M; Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT 3305., Patel-King RS; Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT 3305., Balsbaugh JL; Proteomics and Metabolomics Facility, Center for Open Research Resources & Equipment, University of Connecticut, Storrs, CT 06269. |
المصدر: | Molecular biology of the cell [Mol Biol Cell] 2024 Jul 01; Vol. 35 (7), pp. ar89. Date of Electronic Publication: 2024 May 02. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: American Society for Cell Biology Country of Publication: United States NLM ID: 9201390 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1939-4586 (Electronic) Linking ISSN: 10591524 NLM ISO Abbreviation: Mol Biol Cell Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Bethesda, MD : American Society for Cell Biology, c1992- |
مواضيع طبية MeSH: | Cilia*/metabolism , S-Adenosylmethionine*/metabolism , Protein Processing, Post-Translational* , Proteomics*/methods, Methylation ; Animals ; Humans ; Methyltransferases/metabolism ; S-Adenosylhomocysteine/metabolism ; Epigenome |
مستخلص: | Cilia are highly complex motile, sensory, and secretory organelles that contain perhaps 1000 or more distinct protein components, many of which are subject to various posttranslational modifications such as phosphorylation, N-terminal acetylation, and proteolytic processing. Another common modification is the addition of one or more methyl groups to the side chains of arginine and lysine residues. These tunable additions delocalize the side-chain charge, decrease hydrogen bond capacity, and increase both bulk and hydrophobicity. Methylation is usually mediated by S-adenosylmethionine (SAM)-dependent methyltransferases and reversed by demethylases. Previous studies have identified several ciliary proteins that are subject to methylation including axonemal dynein heavy chains that are modified by a cytosolic methyltransferase. Here, we have performed an extensive proteomic analysis of multiple independently derived cilia samples to assess the potential for SAM metabolism and the extent of methylation in these organelles. We find that cilia contain all the enzymes needed for generation of the SAM methyl donor and recycling of the S-adenosylhomocysteine and tetrahydrofolate byproducts. In addition, we find that at least 155 distinct ciliary proteins are methylated, in some cases at multiple sites. These data provide a comprehensive resource for studying the consequences of methyl marks on ciliary biology. |
References: | Cell. 1981 Dec;27(2 Pt 1):331-40. (PMID: 6460559) J Cell Biol. 2011 Jul 25;194(2):165-75. (PMID: 21788366) Nat Methods. 2022 Jun;19(6):679-682. (PMID: 35637307) Nat Struct Mol Biol. 2021 Jan;28(1):29-37. (PMID: 33318703) New Phytol. 2021 Nov;232(3):1323-1336. (PMID: 34292600) Curr Top Dev Biol. 2008;85:63-82. (PMID: 19147002) J Cell Biol. 2019 Jun 3;218(6):2051-2070. (PMID: 31092556) PLoS One. 2019 May 16;14(5):e0216705. (PMID: 31095607) Cell Motil Cytoskeleton. 2009 Aug;66(8):650-60. (PMID: 19472373) Curr Biol. 2013 May 20;23(10):906-11. (PMID: 23623554) J Cell Biol. 2006 Jun 5;173(5):743-54. (PMID: 16754958) Cell Mol Life Sci. 2017 Sep;74(18):3305-3315. (PMID: 28364192) Nat Genet. 2012 Mar 04;44(4):381-9, S1-2. (PMID: 22387996) Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):E7176-E7184. (PMID: 27803321) J Cell Sci. 2019 Feb 11;132(3):. (PMID: 30659111) Proc Natl Acad Sci U S A. 2022 Aug 2;119(31):e2206098119. (PMID: 35878031) Nat Rev Mol Cell Biol. 2002 Nov;3(11):813-25. (PMID: 12415299) Nat Rev Mol Cell Biol. 2017 Aug;18(8):517-527. (PMID: 28512349) Genome Biol. 2005;6(8):227. (PMID: 16086857) Mol Biol Cell. 2022 Jun 1;33(7):br10. (PMID: 35389765) J Chem Inf Model. 2018 Jul 23;58(7):1406-1414. (PMID: 29927239) Chem Rev. 2018 Jul 25;118(14):6656-6705. (PMID: 29927582) Cell. 2019 Oct 31;179(4):909-922.e12. (PMID: 31668805) J Cell Biol. 1984 Jan;98(1):97-107. (PMID: 6707098) Nat Struct Mol Biol. 2022 May;29(5):472-482. (PMID: 35578022) J Cell Biol. 1992 Dec;119(6):1605-11. (PMID: 1281816) Nature. 2021 Aug;596(7873):583-589. (PMID: 34265844) Nat Rev Mol Cell Biol. 2020 Jun;21(6):307-326. (PMID: 32107477) Biochemistry. 2019 Jan 22;58(3):166-170. (PMID: 30406995) Science. 2006 Aug 18;313(5789):944-8. (PMID: 16917055) J Cell Biol. 2005 Jul 4;170(1):103-13. (PMID: 15998802) Cells. 2023 Oct 20;12(20):. (PMID: 37887336) Curr Biol. 2012 Mar 6;22(5):414-9. (PMID: 22326026) Annu Rev Cell Dev Biol. 2022 Oct 6;38:103-123. (PMID: 35767872) Cell Motil Cytoskeleton. 2002 Jul;52(3):131-43. (PMID: 12112141) Mol Biol Cell. 2008 Oct;19(10):4319-27. (PMID: 18701702) J Biol Chem. 1994 Feb 18;269(7):5452-7. (PMID: 7508939) Eukaryot Cell. 2009 Jul;8(7):922-32. (PMID: 19429781) Cell Mol Life Sci. 2019 Jun;76(12):2329-2348. (PMID: 30879092) Database (Oxford). 2009;2009:bap022. (PMID: 20428338) Cold Spring Harb Perspect Biol. 2017 Jan 3;9(1):. (PMID: 27663773) Mol Cell Proteomics. 2014 Jul;13(7):1769-86. (PMID: 24741115) Mol Biol Cell. 2017 May 1;28(9):1208-1222. (PMID: 28298486) PLoS Biol. 2019 Dec 6;17(12):e3000566. (PMID: 31809498) Nature. 2023 Jan;613(7945):759-766. (PMID: 36631611) Proc Natl Acad Sci U S A. 2024 Jan 30;121(5):e2318522121. (PMID: 38261620) Curr Biol. 2018 Apr 23;28(8):R421-R434. (PMID: 29689227) Biochemistry. 2013 Nov 26;52(47):8501-9. (PMID: 24152136) Nat Rev Mol Cell Biol. 2017 Sep;18(9):533-547. (PMID: 28698599) Mol Cell Proteomics. 2002 Jun;1(6):451-65. (PMID: 12169685) Nat Cell Biol. 2018 Nov;20(11):1250-1255. (PMID: 30323187) J Proteome Res. 2017 Apr 7;16(4):1579-1592. (PMID: 28282151) J Proteome Res. 2017 Jul 7;16(7):2410-2418. (PMID: 28534617) Nat Struct Mol Biol. 2021 Jan;28(1):20-28. (PMID: 33318704) |
معلومات مُعتمدة: | R35 GM140631 United States GM NIGMS NIH HHS; S10 OD028445 United States OD NIH HHS |
المشرفين على المادة: | 7LP2MPO46S (S-Adenosylmethionine) EC 2.1.1.- (Methyltransferases) 979-92-0 (S-Adenosylhomocysteine) |
تواريخ الأحداث: | Date Created: 20240502 Date Completed: 20240606 Latest Revision: 20240714 |
رمز التحديث: | 20240714 |
مُعرف محوري في PubMed: | PMC11244166 |
DOI: | 10.1091/mbc.E24-03-0130 |
PMID: | 38696262 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1939-4586 |
---|---|
DOI: | 10.1091/mbc.E24-03-0130 |