دورية أكاديمية
Transmural Flow Upregulates PD-L1 Expression in Microvascular Networks.
| العنوان: | Transmural Flow Upregulates PD-L1 Expression in Microvascular Networks. |
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| المؤلفون: | Wan Z; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, 02215, USA., Zhang S; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; State Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, 100101, China., Zhong AX; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Xu L; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, 02139, USA., Coughlin MF; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Pavlou G; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Shelton SE; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, 02215, USA., Nguyen HT; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Hirose S; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Kim S; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Floryan MA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Barbie DA; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, 02215, USA., Hodi FS; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, 02215, USA.; Parker Institute for Cancer Immunotherapy, Boston, MA, 02215, USA., Kamm RD; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. |
| المصدر: | Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Jul; Vol. 11 (26), pp. e2400921. Date of Electronic Publication: 2024 May 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: WILEY-VCH Country of Publication: Germany NLM ID: 101664569 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2198-3844 (Electronic) Linking ISSN: 21983844 NLM ISO Abbreviation: Adv Sci (Weinh) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Weinheim : WILEY-VCH, [2014]- |
| مواضيع طبية MeSH: | B7-H1 Antigen*/metabolism , B7-H1 Antigen*/genetics , Up-Regulation* , Microvessels*/metabolism, Humans ; Tumor Microenvironment ; Mice ; Animals ; Endothelial Cells/metabolism |
| مستخلص: | Endothelial programmed death-ligand 1 (PD-L1) expression is higher in tumors than in normal tissues. Also, tumoral vasculatures tend to be leakier than normal vessels leading to a higher trans-endothelial or transmural fluid flow. However, it is not clear whether such elevated transmural flow can control endothelial PD-L1 expression. Here, a new microfluidic device is developed to investigate the relationship between transmural flow and PD-L1 expression in microvascular networks (MVNs). After treating the MVNs with transmural flow for 24 h, the expression of PD-L1 in endothelial cells is upregulated. Additionally, CD8 T cell activation by phytohemagglutinin (PHA) is suppressed when cultured in the MVNs pre-conditioned with transmural flow. Moreover, transmural flow is able to further increase PD-L1 expression in the vessels formed in the tumor microenvironment. Finally, by utilizing blocking antibodies and knock-out assays, it is found that transmural flow-driven PD-L1 upregulation is controlled by integrin α (© 2024 The Authors. Advanced Science published by Wiley‐VCH GmbH.) |
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| معلومات مُعتمدة: | P30 CA014051 United States CA NCI NIH HHS; National Cancer Institute Tissue Engineering Consortium; K00 CA212227 United States CA NCI NIH HHS; P30-CA14051 Koch Institute Support; U01 CA214381 United States CA NCI NIH HHS; SNSF-P400PB_186779 Switzerland SNSF_ Swiss National Science Foundation; K00CA212227 United States CA NCI NIH HHS |
| فهرسة مساهمة: | Keywords: PD‐L1; integrin αVβ3; microfluidic; microvasculature; transmural flow; tumor microenvironment |
| المشرفين على المادة: | 0 (B7-H1 Antigen) 0 (CD274 protein, human) |
| تواريخ الأحداث: | Date Created: 20240502 Date Completed: 20240710 Latest Revision: 20240712 |
| رمز التحديث: | 20240712 |
| مُعرف محوري في PubMed: | PMC11234398 |
| DOI: | 10.1002/advs.202400921 |
| PMID: | 38696611 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2198-3844 |
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| DOI: | 10.1002/advs.202400921 |