دورية أكاديمية
Dysregulation of phosphoenolpyruvate carboxykinase in cancers: A comprehensive analysis.
| العنوان: | Dysregulation of phosphoenolpyruvate carboxykinase in cancers: A comprehensive analysis. |
|---|---|
| المؤلفون: | Xue S; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China., Cai Y; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China., Liu J; Department of Neurosurgery, The 2(nd) affiliated hospital Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330000, China., Ji K; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China., Yi P; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China., Long H; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China., Zhang X; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: zxa@smu.edu.cn., Li P; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: zhimeng_li@163.com., Song Y; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: songye@smu.edu.cn. |
| المصدر: | Cellular signalling [Cell Signal] 2024 Aug; Vol. 120, pp. 111198. Date of Electronic Publication: 2024 Apr 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 8904683 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3913 (Electronic) Linking ISSN: 08986568 NLM ISO Abbreviation: Cell Signal Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Elsevier Science Ltd Original Publication: Oxford ; New York : Pergamon Press, 1988- |
| مواضيع طبية MeSH: | Neoplasms*/metabolism , Neoplasms*/genetics , Neoplasms*/pathology , Phosphoenolpyruvate Carboxykinase (GTP)*/metabolism , Phosphoenolpyruvate Carboxykinase (GTP)*/genetics, Humans ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic ; Phosphoenolpyruvate Carboxykinase (ATP)/metabolism ; Phosphoenolpyruvate Carboxykinase (ATP)/genetics ; Animals ; Intracellular Signaling Peptides and Proteins/metabolism ; Intracellular Signaling Peptides and Proteins/genetics ; Mice ; Prognosis ; Cell Proliferation |
| مستخلص: | Background: Phosphoenolpyruvate carboxykinase (PEPCK) plays a crucial role in gluconeogenesis, glycolysis, and the tricarboxylic acid cycle by converting oxaloacetate into phosphoenolpyruvate. Two distinct isoforms of PEPCK, specifically cytosolic PCK1 and mitochondrial PCK2, have been identified. Nevertheless, the comprehensive understanding of their dysregulation in pan-cancer and their potential mechanism contributing to signaling transduction pathways remains elusive. Methods: We conducted comprehensive analyses of PEPCK gene expression across 33 diverse cancer types using data from The Cancer Genome Atlas (TCGA). Multiple public databases such as HPA, TIMER 2.0, GEPIA2, cBioPortal, UALCAN, CancerSEA, and String were used to investigate protein levels, prognostic significance, clinical associations, genetic mutations, immune cell infiltration, single-cell sequencing, and functional enrichment analysis in patients with pan-cancer. PEPCK expression was analyzed about different clinical and genetic factors of patients using data from TCGA, GEO, and CGGA databases. Furthermore, the role of PCK2 in Glioma was examined using both in vitro and in vivo experiments. Results: The analysis we conducted revealed that the expression of PEPCK is involved in both clinical outcomes and immune cell infiltration. Initially, we verified the high expression of PCK2 in GBM cells and its role in metabolic reprogramming and proliferation in GBM. Conclusion: Our study showed a correlation between PEPCK (PCK1 and PCK2) expression with clinical prognosis, gene mutation, and immune infiltrates. These findings identified two possible predictive biomarkers across different cancer types, as well as a comprehensive analysis of PCK2 expression in various tumors, with a focus on GBM. Competing Interests: Declaration of competing interest The researchers declare no conflict of interest. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Glioblastoma; Immune infiltration; Metabolism; PCK1; PCK2; Pan-cancer |
| المشرفين على المادة: | EC 4.1.1.49 (PCK2 protein, human) EC 4.1.1.32 (Phosphoenolpyruvate Carboxykinase (GTP)) EC 4.1.1.32 (PCK1 protein, human) EC 4.1.1.49 (Phosphoenolpyruvate Carboxykinase (ATP)) 0 (Intracellular Signaling Peptides and Proteins) |
| تواريخ الأحداث: | Date Created: 20240502 Date Completed: 20240611 Latest Revision: 20240621 |
| رمز التحديث: | 20240621 |
| DOI: | 10.1016/j.cellsig.2024.111198 |
| PMID: | 38697449 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3913 |
|---|---|
| DOI: | 10.1016/j.cellsig.2024.111198 |