دورية أكاديمية
Staphylococcus aureus Isolated From Traditional Artisanal Raw Milk Cheese from Southern Brazil: Diversity, Virulence, and Antimicrobial Resistance Profile.
| العنوان: | Staphylococcus aureus Isolated From Traditional Artisanal Raw Milk Cheese from Southern Brazil: Diversity, Virulence, and Antimicrobial Resistance Profile. |
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| المؤلفون: | Aguiar RAC; Food Technology and Bioprocesses Research Group, Department of Food Science and Technology, Federal University of Santa Catarina (UFSC), 88034-001 Florianópolis, SC, Brazil., Ferreira FA; Department of Microbiology, Immunology, and Parasitology, UFSC, 88040-970 Florianópolis, SC, Brazil., Rubio Cieza MY; Department of Food Science and Nutrition, School of Food Engineering (FEA), State University of Campinas (UNICAMP), 13083-862 Campinas, SP, Brazil., Silva NCC; Department of Food Science and Nutrition, School of Food Engineering (FEA), State University of Campinas (UNICAMP), 13083-862 Campinas, SP, Brazil., Miotto M; Food Technology and Bioprocesses Research Group, Department of Food Science and Technology, Federal University of Santa Catarina (UFSC), 88034-001 Florianópolis, SC, Brazil., Carvalho MM; Food Technology and Bioprocesses Research Group, Department of Food Science and Technology, Federal University of Santa Catarina (UFSC), 88034-001 Florianópolis, SC, Brazil., Bazzo BR; Neoprospecta Microbiome Technologies, 88054-700 Florianópolis, SC, Brazil., Botelho LAB; Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro (UFRJ), 21941-902 Rio de Janeiro, RJ, Brazil., Dias RS; Ezequiel Dias Foundation (FUNED), Enterotoxins Laboratory, Public Health Center of the State of Minas Gerais, 30510-010 Belo Horizonte, MG, Brazil., De Dea Lindner J; Food Technology and Bioprocesses Research Group, Department of Food Science and Technology, Federal University of Santa Catarina (UFSC), 88034-001 Florianópolis, SC, Brazil. Electronic address: juliano.lindner@ufsc.br. |
| المصدر: | Journal of food protection [J Food Prot] 2024 Jun; Vol. 87 (6), pp. 100285. Date of Electronic Publication: 2024 Apr 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 7703944 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-9097 (Electronic) Linking ISSN: 0362028X NLM ISO Abbreviation: J Food Prot Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [New York, NY] : Elsevier Original Publication: Ames, Iowa, International Association of Milk, Food, and Environmental Sanitarians. |
| مواضيع طبية MeSH: | Cheese*/microbiology , Milk*/microbiology , Staphylococcus aureus*/drug effects , Anti-Bacterial Agents*/pharmacology, Brazil ; Animals ; Microbial Sensitivity Tests ; Virulence ; Food Microbiology ; Humans ; Drug Resistance, Bacterial ; Food Contamination/analysis ; Enterotoxins/genetics |
| مستخلص: | Staphylococcus aureus is one of the primary pathogenic agents found in cheeses produced with raw milk. Some strains of S. aureus are enterotoxigenic, possessing the ability to produce toxins responsible for staphylococcal food poisoning when present in contaminated foods. This study aimed to genotypically characterize, assess the antimicrobial resistance profile, and examine the enterotoxigenic potential of strains of S. aureus isolated from artisanal colonial cheese. Additionally, a bacterial diversity assessment in the cheeses was conducted by sequencing the 16S rRNA gene. The metataxomic profile revealed the presence of 68 distinct species in the cheese samples. Fifty-seven isolates of S. aureus were identified, with highlighted resistance to penicillin in 33% of the isolates, followed by clindamycin (28%), erythromycin (26%), and tetracycline (23%). The evaluated strains also exhibited inducible resistance to clindamycin, with nine isolates considered multidrug-resistant (MDR). The agr type I was the most prevalent (62%) among the isolates, followed by agr type II (24%). Additionally, ten spa types were identified. Although no enterotoxins and their associated genes were detected in the samples and isolates, respectively, the Panton-Valentine leukocidin gene (lukS-lukF) was found in 39% of the isolates. The presence of MDR pathogens in the artisanal raw milk cheese production chain underscores the need for quality management to prevent the contamination and dissemination of S. aureus strains. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antimicrobial multidrug resistance; Food safety; One Health; Raw milk cheese; Staphylococcal enterotoxins |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Enterotoxins) |
| تواريخ الأحداث: | Date Created: 20240502 Date Completed: 20240526 Latest Revision: 20240526 |
| رمز التحديث: | 20240527 |
| DOI: | 10.1016/j.jfp.2024.100285 |
| PMID: | 38697483 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1944-9097 |
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| DOI: | 10.1016/j.jfp.2024.100285 |