دورية أكاديمية
أعرض في EDS

The TNFSF12/TWEAK Modulates Colonic Inflammatory Fibroblast Differentiation and Promotes Fibroblast-Monocyte Interactions.

التفاصيل البيبلوغرافية
العنوان: The TNFSF12/TWEAK Modulates Colonic Inflammatory Fibroblast Differentiation and Promotes Fibroblast-Monocyte Interactions.
المؤلفون: Matellan C; School of Biomolecular and Biomedical Sciences, University College Dublin, Belfield, Dublin, Ireland.; Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland.; School of Medicine, Conway Institute of Biomolecular and biomedical Research, University College Dublin, Belfield, Dublin, Ireland., Kennedy C; Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland.; School of Medicine, Conway Institute of Biomolecular and biomedical Research, University College Dublin, Belfield, Dublin, Ireland.; Diabetes Complications Research Centre, University College Dublin, Belfield, Dublin, Ireland., Santiago-Vela MI; School of Biomolecular and Biomedical Sciences, University College Dublin, Belfield, Dublin, Ireland.; Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland., Hochegger J; School of Biomolecular and Biomedical Sciences, University College Dublin, Belfield, Dublin, Ireland.; Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland., Ní Chathail MB; Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland.; School of Public Health, Physiotherapy, and Sports Science, University College Dublin, Belfield, Dublin, Ireland., Wu A; Department of Pediatrics, School of Medicine, University of California San Diego, La Jolla, CA., Shannon C; Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland.; School of Medicine, Conway Institute of Biomolecular and biomedical Research, University College Dublin, Belfield, Dublin, Ireland., Roche HM; Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland.; School of Public Health, Physiotherapy, and Sports Science, University College Dublin, Belfield, Dublin, Ireland.; Institute for Global Food Security, Queen's University Belfast, Belfast, U.K., Aceves SS; Department of Pediatrics, School of Medicine, University of California San Diego, La Jolla, CA.; Rady Children's Hospital, San Diego, CA., Godson C; Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland.; School of Medicine, Conway Institute of Biomolecular and biomedical Research, University College Dublin, Belfield, Dublin, Ireland.; Diabetes Complications Research Centre, University College Dublin, Belfield, Dublin, Ireland., Manresa MC; School of Biomolecular and Biomedical Sciences, University College Dublin, Belfield, Dublin, Ireland.; Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland.
المصدر: Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2024 Jun 15; Vol. 212 (12), pp. 1958-1970.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Association of Immunologists Country of Publication: United States NLM ID: 2985117R Publication Model: Print Cited Medium: Internet ISSN: 1550-6606 (Electronic) Linking ISSN: 00221767 NLM ISO Abbreviation: J Immunol Subsets: MEDLINE
أسماء مطبوعة: Publication: Bethesda, MD : American Association of Immunologists
Original Publication: Baltimore : Williams & Wilkins, c1950-
مواضيع طبية MeSH: Cytokine TWEAK*/metabolism , Monocytes*/immunology , Monocytes*/metabolism , Fibroblasts*/metabolism , Fibroblasts*/immunology , Colon*/immunology , Colon*/pathology , Colon*/metabolism , Cell Differentiation*/immunology, Humans ; Cell Communication/immunology ; Inflammation/immunology ; THP-1 Cells ; Coculture Techniques ; Cytokines/metabolism ; Cell Adhesion
مستخلص: Fibroblasts acquire a proinflammatory phenotype in inflammatory bowel disease, but the factors driving this process and how fibroblasts contribute to mucosal immune responses are incompletely understood. TNF superfamily member 12 (TNFSF12, or TNF-like weak inducer of apoptosis [TWEAK]) has gained interest as a mediator of chronic inflammation. In this study, we explore its role as a driver of inflammatory responses in fibroblasts and its contribution to fibroblast-monocyte interaction using human primary colonic fibroblasts, THP-1 and primary monocytes. Recombinant human TWEAK induced the expression of cytokines, chemokines, and immune receptors in primary colonic fibroblasts. The TWEAK upregulated transcriptome shared 29% homology with a previously published transcriptional profile of inflammatory fibroblasts from ulcerative colitis. TWEAK elevated surface expression of activated fibroblast markers and adhesion molecules (podoplanin [PDPN], ICAM-1, and VCAM-1) and secretion of IL-6, CCL2, and CXCL10. In coculture, fibroblasts induced monocyte adhesion and secretion of CXCL1 and IL-8, and they promoted a CD14high/ICAM-1high phenotype in THP-1 cells, which was enhanced when fibroblasts were prestimulated with TWEAK. Primary monocytes in coculture with TWEAK-treated fibroblasts had altered surface expression of CD16 and triggering receptor expressed on myeloid cells-1 (TREM-1) as well as increased CXCL1 and CXCL10 secretion. Conversely, inhibition of the noncanonical NF-κB pathway on colonic fibroblasts with a NF-κB-inducing kinase small molecule inhibitor impaired their ability to induce a CD14high phenotype on monocytes. Our results indicate that TWEAK promotes an inflammatory fibroblast-monocyte crosstalk that may be amenable for therapeutic intervention.
(Copyright © 2024 by The American Association of Immunologists, Inc.)
References: Nat Cardiovasc Res. 2022 Aug;1(8):761-774. (PMID: 36092510)
J Leukoc Biol. 2012 Aug;92(2):265-79. (PMID: 22672874)
Cell Immunol. 2014 Sep-Oct;291(1-2):41-8. (PMID: 24726741)
J Clin Invest. 2007 Oct;117(10):3097-106. (PMID: 17853946)
Genome Biol. 2014;15(12):550. (PMID: 25516281)
Cancer Discov. 2019 Aug;9(8):1102-1123. (PMID: 31197017)
Front Immunol. 2018 Nov 27;9:2764. (PMID: 30542349)
Nucleic Acids Res. 2011 Jan;39(Database issue):D19-21. (PMID: 21062823)
Mucosal Immunol. 2013 Nov;6(6):1131-42. (PMID: 23462911)
J Immunol. 2014 Sep 1;193(5):2373-83. (PMID: 25057003)
Bioinformatics. 2013 Jan 1;29(1):15-21. (PMID: 23104886)
Gastroenterology. 2020 Nov;159(5):1778-1792.e13. (PMID: 32712105)
Nature. 2021 May;593(7860):575-579. (PMID: 33981032)
Cell. 2019 Sep 5;178(6):1493-1508.e20. (PMID: 31474370)
Inflamm Bowel Dis. 2016 Sep;22(9):2265-79. (PMID: 27508514)
Nat Commun. 2018 Feb 23;9(1):789. (PMID: 29476097)
Curr Drug Targets. 2021;22(7):760-769. (PMID: 33475057)
Nat Med. 2021 Nov;27(11):1970-1981. (PMID: 34675383)
Front Immunol. 2022 Jul 01;13:922111. (PMID: 35844494)
BMJ Open. 2023 Mar 28;13(3):e065186. (PMID: 36977543)
J Exp Med. 2017 Nov 6;214(11):3311-3329. (PMID: 28970239)
Cell Rep. 2019 Mar 26;26(13):3698-3708.e5. (PMID: 30917322)
J Immunol. 2005 Jun 15;174(12):8173-82. (PMID: 15944326)
Med. 2022 Jul 8;3(7):481-518.e14. (PMID: 35649411)
Nat Biotechnol. 2020 Mar;38(3):276-278. (PMID: 32055031)
Genome Biol. 2019 Dec 23;20(1):296. (PMID: 31870423)
Cell. 2018 Oct 4;175(2):372-386.e17. (PMID: 30270042)
Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):E3173-E3181. (PMID: 29559533)
EBioMedicine. 2019 Feb;40:733-742. (PMID: 30685385)
Nat Rev Rheumatol. 2020 Jun;16(6):316-333. (PMID: 32393826)
J Immunol. 2010 Aug 1;185(3):1593-605. (PMID: 20610643)
Nat Med. 2017 May;23(5):579-589. (PMID: 28368383)
Nat Rev Gastroenterol Hepatol. 2023 Aug;20(8):538-553. (PMID: 37069320)
Arthritis Res Ther. 2019 Jul 8;21(1):169. (PMID: 31287012)
Curr Protoc. 2021 Mar;1(3):e90. (PMID: 33780170)
PLoS Biol. 2020 Dec 11;18(12):e3001032. (PMID: 33306673)
Bioinformatics. 2016 Oct 1;32(19):3047-8. (PMID: 27312411)
Cell Mol Gastroenterol Hepatol. 2019;8(3):427-446. (PMID: 31181286)
J Immunol. 2022 Oct 26;:. (PMID: 36288906)
Mucosal Immunol. 2022 Feb;15(2):327-337. (PMID: 34903876)
J Cell Physiol. 2018 Feb;233(2):968-978. (PMID: 28383766)
Arthritis Res Ther. 2006;8(5):R146. (PMID: 16945157)
Gastroenterology. 2008 Jan;134(1):156-65. (PMID: 18166353)
Gastroenterology. 2005 Aug;129(2):626-38. (PMID: 16083717)
Sci Rep. 2022 Jan 10;12(1):452. (PMID: 35013585)
Nat Methods. 2017 Apr;14(4):417-419. (PMID: 28263959)
F1000Res. 2021 Sep 28;10:979. (PMID: 35814628)
J Crohns Colitis. 2021 Aug 2;15(8):1346-1361. (PMID: 33537747)
Nature. 2019 Jun;570(7760):246-251. (PMID: 31142839)
Nat Commun. 2017 Jan 16;8:14049. (PMID: 28091601)
J Physiol. 2023 Jun;601(12):2273-2291. (PMID: 37062932)
J Immunol. 2010 Mar 1;184(5):2671-6. (PMID: 20107182)
Gastroenterology. 2011 Jul;141(1):259-68, 268.e1-8. (PMID: 21440550)
J Immunol. 2001 Dec 1;167(11):6330-7. (PMID: 11714797)
Nat Commun. 2018 Jan 12;9(1):179. (PMID: 29330524)
Nat Rev Immunol. 2017 Sep;17(9):545-558. (PMID: 28580957)
Gastroenterology. 2017 Feb;152(2):340-350.e6. (PMID: 27720839)
Inflamm Bowel Dis. 2007 May;13(5):566-72. (PMID: 17260384)
معلومات مُعتمدة: 21/PATH-S/9621 Science Foundation Ireland (SFI); 15/US/B3130 Science Foundation Ireland (SFI); 15/IA/3152 Science Foundation Ireland (SFI); GOIPD/2023/1118 Irish Research Council (An Chomhairle um Thaighde in Éirinn)
المشرفين على المادة: 0 (Cytokine TWEAK)
0 (TNFSF12 protein, human)
0 (Cytokines)
تواريخ الأحداث: Date Created: 20240503 Date Completed: 20240603 Latest Revision: 20240611
رمز التحديث: 20240611
مُعرف محوري في PubMed: PMC11149899
DOI: 10.4049/jimmunol.2300762
PMID: 38700420
قاعدة البيانات: MEDLINE
الوصف
تدمد:1550-6606
DOI:10.4049/jimmunol.2300762