دورية أكاديمية
A novel vaccine strategy against Brucellosis using Brucella abortus multi-epitope OMPs vaccine based on Lactococcus lactis live bacterial vectors.
| العنوان: | A novel vaccine strategy against Brucellosis using Brucella abortus multi-epitope OMPs vaccine based on Lactococcus lactis live bacterial vectors. |
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| المؤلفون: | Piri-Gharaghie T; Biotechnology Research Center, Faculty of Biological Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran. Electronic address: tohidpirie@yahoo.com., Ghajari G; Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran., Rezaeizadeh G; Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran., Adil M; Pharmacy College, Al-Farahidi University, Baghdad, Iraq., Mahdi MH; College of Pharmacy, Ahl Al Bayt University, Kerbala, Iraq. |
| المصدر: | International immunopharmacology [Int Immunopharmacol] 2024 Jun 15; Vol. 134, pp. 112204. Date of Electronic Publication: 2024 May 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 100965259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1705 (Electronic) Linking ISSN: 15675769 NLM ISO Abbreviation: Int Immunopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier Science, c2001- |
| مواضيع طبية MeSH: | Brucella abortus*/immunology , Brucellosis*/prevention & control , Brucellosis*/immunology , Lactococcus lactis*/genetics , Lactococcus lactis*/immunology , Brucella Vaccine*/immunology , Brucella Vaccine*/administration & dosage , Brucella Vaccine*/genetics , Mice, Inbred BALB C*, Animals ; Mice ; Female ; Bacterial Outer Membrane Proteins/immunology ; Bacterial Outer Membrane Proteins/genetics ; Epitopes/immunology ; Antibodies, Bacterial/blood ; Antibodies, Bacterial/immunology ; Spleen/immunology ; Genetic Vectors ; Immunoglobulin G/blood ; Immunoglobulin G/immunology ; Cytokines/metabolism |
| مستخلص: | Brucella infections typically occur in mucosal membranes, emphasizing the need for mucosal vaccinations. This study evaluated the effectiveness of orally administering Lactococcus lactis (L. lactis) for producing the Brucella abortus multi-epitope OMPs peptide. A multi-epitope plasmid was generated through a reverse vaccinology method, and mice were administered the genetically modified L. lactis orally as a vaccine. The plasmid underwent digestion, synthesizing a 39 kDa-sized protein known as OMPs by the target group. The sera of mice that were administered the pNZ8124-OMPs-L. lactis vaccine exhibited a notable presence of IgG1 antibodies specific to outer membrane proteins (OMPs), heightened levels of interferon (IFN-λ) and tumor necrosis factor alpha (TNF-α), and enhanced transcription rates of interleukin 4 (IL-4) and interleukin 10 (IL-10). The spleen sections from the pNZ8124-OMPs-L. lactis and IRIBA group had less morphological damage associated with inflammation, infiltration of lymphocytes, and lesions to the spleen. The findings present a novel approach to utilizing the food-grade, non-pathogenic L. lactis as a protein cell factory to synthesize innovative immunological candidate OMPs. This approach offers a distinctive way to evaluate experimental medicinal items' practicality, safety, affordability, and long-term sustainability. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Brucellosis infection; Live bacterial vectors; Mucosal vaccination; Protein delivery vehicles; Reverse vaccinology |
| المشرفين على المادة: | 0 (Brucella Vaccine) 0 (Bacterial Outer Membrane Proteins) 0 (Epitopes) 0 (Antibodies, Bacterial) 0 (Immunoglobulin G) 0 (Cytokines) |
| تواريخ الأحداث: | Date Created: 20240504 Date Completed: 20240602 Latest Revision: 20240602 |
| رمز التحديث: | 20240602 |
| DOI: | 10.1016/j.intimp.2024.112204 |
| PMID: | 38703567 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-1705 |
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| DOI: | 10.1016/j.intimp.2024.112204 |