دورية أكاديمية
Deorphanizing an odorant receptor tuned to palm tree volatile esters in the Asian palm weevil sheds light on the mechanisms of palm tree selection.
| العنوان: | Deorphanizing an odorant receptor tuned to palm tree volatile esters in the Asian palm weevil sheds light on the mechanisms of palm tree selection. |
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| المؤلفون: | Antony B; King Saud University, Chair of Date Palm Research, Center for Chemical Ecology and Functional Genomics, Department of Plant Protection, College of Food and Agricultural Sciences, Riyadh, 11451, Saudi Arabia. Electronic address: bantony@ksu.edu.sa., Montagné N; INRAE, Sorbonne Université, CNRS, IRD, UPEC, Université Paris Cité, Institute of Ecology and Environmental Sciences of Paris, iEES-Paris, 78000, Versailles, France., Comte A; INRAE, Sorbonne Université, CNRS, IRD, UPEC, Université Paris Cité, Institute of Ecology and Environmental Sciences of Paris, iEES-Paris, 78000, Versailles, France., Mfarrej S; King Abdullah University of Science and Technology (KAUST), Bioscience Programme, BESE Division, Thuwal, Jeddah, 23955-6900, Saudi Arabia., Jakše J; University of Ljubljana, Biotechnical Faculty, Agronomy Department, SI-1000, Ljubljana, Slovenia., Capoduro R; INRAE, Sorbonne Université, CNRS, IRD, UPEC, Université Paris Cité, Institute of Ecology and Environmental Sciences of Paris, iEES-Paris, 78000, Versailles, France., Shelke R; Don Bosco College of Agriculture, Agricultural Entomology Department, Sulcorna, Goa, 403705, India., Cali K; The University of Manchester, Department of Chemical Engineering, Manchester, M13 9PL, UK., AlSaleh MA; King Saud University, Chair of Date Palm Research, Center for Chemical Ecology and Functional Genomics, Department of Plant Protection, College of Food and Agricultural Sciences, Riyadh, 11451, Saudi Arabia., Persaud K; The University of Manchester, Department of Chemical Engineering, Manchester, M13 9PL, UK., Pain A; King Abdullah University of Science and Technology (KAUST), Bioscience Programme, BESE Division, Thuwal, Jeddah, 23955-6900, Saudi Arabia., Jacquin-Joly E; INRAE, Sorbonne Université, CNRS, IRD, UPEC, Université Paris Cité, Institute of Ecology and Environmental Sciences of Paris, iEES-Paris, 78000, Versailles, France. |
| المصدر: | Insect biochemistry and molecular biology [Insect Biochem Mol Biol] 2024 Jun; Vol. 169, pp. 104129. Date of Electronic Publication: 2024 May 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: England NLM ID: 9207282 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0240 (Electronic) Linking ISSN: 09651748 NLM ISO Abbreviation: Insect Biochem Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2003->: Amsterdam : Elsevier Science Original Publication: Oxford [England] ; New York : Pergamon Press, c1992- |
| مواضيع طبية MeSH: | Weevils*/metabolism , Weevils*/genetics , Receptors, Odorant*/metabolism , Receptors, Odorant*/genetics , Receptors, Odorant*/chemistry, Animals ; Volatile Organic Compounds/metabolism ; Male ; Phylogeny ; Female ; Arecaceae/metabolism ; Insect Proteins/metabolism ; Insect Proteins/genetics ; Insect Proteins/chemistry ; Arthropod Antennae/metabolism ; Esters/metabolism |
| مستخلص: | The Asian palm weevil, Rhynchophorus ferrugineus, is a tremendously important agricultural pest primarily adapted to palm trees and causes severe destruction, threatening sustainable palm cultivation worldwide. The host plant selection of this weevil is mainly attributed to the functional specialization of odorant receptors (ORs) that detect palm-derived volatiles. Yet, ligands are known for only two ORs of R. ferrugineus, and we still lack information on the mechanisms of palm tree detection. This study identified a highly expressed antennal R. ferrugineus OR, RferOR2, thanks to newly generated transcriptomic data. The phylogenetic analysis revealed that RferOR2 belongs to the major coleopteran OR group 2A and is closely related to a sister clade containing an R. ferrugineus OR (RferOR41) tuned to the non-host plant volatile and antagonist, α-pinene. Functional characterization of RferOR2 via heterologous expression in Drosophila olfactory neurons revealed that this receptor is tuned to several ecologically relevant palm-emitted odors, most notably ethyl and methyl ester compounds, but not to any of the pheromone compounds tested, including the R. ferrugineus aggregation pheromone. We did not evidence any differential expression of RferOR2 in the antennae of both sexes, suggesting males and females detect these compounds equally. Next, we used the newly identified RferOR2 ligands to demonstrate that including synthetic palm ester volatiles as single compounds and in combinations in pheromone-based mass trapping has a synergistic attractiveness effect to R. ferrugineus aggregation pheromone, resulting in significantly increased weevil catches. Our study identified a key OR from a palm weevil species tuned to several ecologically relevant palm volatiles and represents a significant step forward in understanding the chemosensory mechanisms of host detection in palm weevils. Our study also defines RferOR2 as an essential model for exploring the molecular basis of host detection in other palm weevil species. Finally, our work showed that insect OR deorphanization could aid in identifying novel behaviorally active volatiles that can interfere with weevil host-searching behavior in sustainable pest management applications. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Deorphanization; Odorant receptor; Palm esters; Palm weevil; Sustainable palm agriculture; Synergistic effect |
| تواريخ الأحداث: | Date Created: 20240504 Date Completed: 20240519 Latest Revision: 20240519 |
| رمز التحديث: | 20240520 |
| DOI: | 10.1016/j.ibmb.2024.104129 |
| PMID: | 38704126 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0240 |
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| DOI: | 10.1016/j.ibmb.2024.104129 |