دورية أكاديمية
MAIT cell-MR1 reactivity is highly conserved across multiple divergent species.
| العنوان: | MAIT cell-MR1 reactivity is highly conserved across multiple divergent species. |
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| المؤلفون: | Edmans MD; Department of Enhanced Host Responses, The Pirbright Institute, Pirbright, United Kingdom; Peter Medawar Building for Pathogen Research, University of Oxford, Oxford, United Kingdom. Electronic address: matthew.edmans@ndm.ox.ac.uk., Connelley TK; Division of Infection and Immunity, The Roslin Institute, The University of Edinburgh, Roslin, United Kingdom., Morgan S; Department of Enhanced Host Responses, The Pirbright Institute, Pirbright, United Kingdom., Pediongco TJ; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia., Jayaraman S; Division of Infection and Immunity, The Roslin Institute, The University of Edinburgh, Roslin, United Kingdom., Juno JA; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia., Meehan BS; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia., Dewar PM; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia., Maze EA; Department of Enhanced Host Responses, The Pirbright Institute, Pirbright, United Kingdom., Roos EO; Department of Enhanced Host Responses, The Pirbright Institute, Pirbright, United Kingdom., Paudyal B; Department of Enhanced Host Responses, The Pirbright Institute, Pirbright, United Kingdom., Mak JYW; Centre for Chemistry and Drug Discovery, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia; Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia., Liu L; Centre for Chemistry and Drug Discovery, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia., Fairlie DP; Centre for Chemistry and Drug Discovery, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia; Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia., Wang H; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia; State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, Guangzhou Medical University, Guangzhou, China., Corbett AJ; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia., McCluskey J; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia., Benedictus L; Division of Infection and Immunity, The Roslin Institute, The University of Edinburgh, Roslin, United Kingdom; Faculty of Veterinary Medicine, Department of Population Health Sciences, Utrecht University, Utrecht, The Netherlands., Tchilian E; Department of Enhanced Host Responses, The Pirbright Institute, Pirbright, United Kingdom., Klenerman P; Peter Medawar Building for Pathogen Research, University of Oxford, Oxford, United Kingdom., Eckle SBG; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia. Electronic address: seckle@unimelb.edu.au. |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2024 Jun; Vol. 300 (6), pp. 107338. Date of Electronic Publication: 2024 May 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Mucosal-Associated Invariant T Cells*/immunology , Mucosal-Associated Invariant T Cells*/metabolism , Histocompatibility Antigens Class I*/immunology , Histocompatibility Antigens Class I*/metabolism , Minor Histocompatibility Antigens*/metabolism , Minor Histocompatibility Antigens*/genetics , Minor Histocompatibility Antigens*/immunology , Minor Histocompatibility Antigens*/chemistry , Species Specificity*, Animals ; Humans ; Mice ; Cattle ; Swine ; Macaca ; Receptors, Antigen, T-Cell, alpha-beta/immunology ; Receptors, Antigen, T-Cell, alpha-beta/metabolism ; Receptors, Antigen, T-Cell, alpha-beta/genetics |
| مستخلص: | Mucosal-associated invariant T (MAIT) cells are a subset of unconventional T cells that recognize small molecule metabolites presented by major histocompatibility complex class I related protein 1 (MR1), via an αβ T cell receptor (TCR). MAIT TCRs feature an essentially invariant TCR α-chain, which is highly conserved between mammals. Similarly, MR1 is the most highly conserved major histocompatibility complex-I-like molecule. This extreme conservation, including the mode of interaction between the MAIT TCR and MR1, has been shown to allow for species-mismatched reactivities unique in T cell biology, thereby allowing the use of selected species-mismatched MR1-antigen (MR1-Ag) tetramers in comparative immunology studies. However, the pattern of cross-reactivity of species-mismatched MR1-Ag tetramers in identifying MAIT cells in diverse species has not been formally assessed. We developed novel cattle and pig MR1-Ag tetramers and utilized these alongside previously developed human, mouse, and pig-tailed macaque MR1-Ag tetramers to characterize cross-species tetramer reactivities. MR1-Ag tetramers from each species identified T cell populations in distantly related species with specificity that was comparable to species-matched MR1-Ag tetramers. However, there were subtle differences in staining characteristics with practical implications for the accurate identification of MAIT cells. Pig MR1 is sufficiently conserved across species that pig MR1-Ag tetramers identified MAIT cells from the other species. However, MAIT cells in pigs were at the limits of phenotypic detection. In the absence of sheep MR1-Ag tetramers, a MAIT cell population in sheep blood was identified phenotypically, utilizing species-mismatched MR1-Ag tetramers. Collectively, our results validate the use and define the limitations of species-mismatched MR1-Ag tetramers in comparative immunology studies. Competing Interests: Conflict of interest J. Y. W. M., L. L., D. P. F., A. J. C., J. M., and S. B. G. E. are inventors on university owned patent rights (patent families WO/2015/149130 and WO/2014/005194) licensed for commercial use to Immudex and for non-profit use to the NIH Tetramer Core Facility. All other authors declare that they have no conflicts of interest with the contents of this article. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 5-(2-oxopropylideneamino)-6-d-ribitylaminouracil (5-OP-RU); MHC-I related protein 1 (MR1); T cell biology; T cell receptor (TCR); antigen (Ag); comparative immunology; innate-like immunity; major histocompatibility complex (MHC); mucosal-associated invariant T (MAIT) cell |
| المشرفين على المادة: | 0 (Histocompatibility Antigens Class I) 0 (Minor Histocompatibility Antigens) 0 (MR1 protein, human) 0 (Receptors, Antigen, T-Cell, alpha-beta) |
| تواريخ الأحداث: | Date Created: 20240505 Date Completed: 20240702 Latest Revision: 20240723 |
| رمز التحديث: | 20240723 |
| مُعرف محوري في PubMed: | PMC11190491 |
| DOI: | 10.1016/j.jbc.2024.107338 |
| PMID: | 38705391 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1083-351X |
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| DOI: | 10.1016/j.jbc.2024.107338 |