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Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803.

التفاصيل البيبلوغرافية
العنوان: Glycogen synthesis prevents metabolic imbalance and disruption of photosynthetic electron transport from photosystem II during transition to photomixotrophy in Synechocystis sp. PCC 6803.
المؤلفون: Ortega-Martínez P; Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla, Consejo Superior de Investigaciones Científicas, Américo Vespucio 49, Sevilla, 41092, Spain.; Departamento de Bioquímica Vegetal y Biología Molecular, Facultad de Biología, Universidad de Sevilla, Profesor García González s/n, Sevilla, 41012, Spain., Nikkanen L; Molecular Plant Biology, Department of Life Technologies, University of Turku, Turku, FI-20014, Finland., Wey LT; Molecular Plant Biology, Department of Life Technologies, University of Turku, Turku, FI-20014, Finland., Florencio FJ; Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla, Consejo Superior de Investigaciones Científicas, Américo Vespucio 49, Sevilla, 41092, Spain.; Departamento de Bioquímica Vegetal y Biología Molecular, Facultad de Biología, Universidad de Sevilla, Profesor García González s/n, Sevilla, 41012, Spain., Allahverdiyeva Y; Molecular Plant Biology, Department of Life Technologies, University of Turku, Turku, FI-20014, Finland., Díaz-Troya S; Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla, Consejo Superior de Investigaciones Científicas, Américo Vespucio 49, Sevilla, 41092, Spain.; Departamento de Bioquímica Vegetal y Biología Molecular, Facultad de Biología, Universidad de Sevilla, Profesor García González s/n, Sevilla, 41012, Spain.
المصدر: The New phytologist [New Phytol] 2024 Jul; Vol. 243 (1), pp. 162-179. Date of Electronic Publication: 2024 May 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE
أسماء مطبوعة: Publication: Oxford : Wiley on behalf of New Phytologist Trust
Original Publication: London, New York [etc.] Academic Press.
مواضيع طبية MeSH: Synechocystis*/metabolism , Synechocystis*/drug effects , Synechocystis*/growth & development , Synechocystis*/genetics , Glycogen*/metabolism , Photosynthesis* , Photosystem II Protein Complex*/metabolism, Electron Transport ; Mutation/genetics ; Glucose/metabolism ; Carbon Dioxide/metabolism ; Oxygen/metabolism ; Glucose-1-Phosphate Adenylyltransferase/metabolism ; Glucose-1-Phosphate Adenylyltransferase/genetics ; Phosphoglucomutase/metabolism ; Phosphoglucomutase/genetics
مستخلص: Some cyanobacteria can grow photoautotrophically or photomixotrophically by using simultaneously CO 2 and glucose. The switch between these trophic modes and the role of glycogen, their main carbon storage macromolecule, was investigated. We analysed the effect of glucose addition on the physiology, metabolic and photosynthetic state of Synechocystis sp. PCC 6803 and mutants lacking phosphoglucomutase and ADP-glucose pyrophosphorylase, with limitations in glycogen synthesis. Glycogen acted as a metabolic buffer: glucose addition increased growth and glycogen reserves in the wild-type (WT), but arrested growth in the glycogen synthesis mutants. Already 30 min after glucose addition, metabolites from the Calvin-Benson-Bassham cycle and the oxidative pentose phosphate shunt increased threefold more in the glycogen synthesis mutants than the WT. These alterations substantially affected the photosynthetic performance of the glycogen synthesis mutants, as O 2 evolution and CO 2 uptake were both impaired. We conclude that glycogen synthesis is essential during transitions to photomixotrophy to avoid metabolic imbalance that induces inhibition of electron transfer from PSII and subsequently accumulation of reactive oxygen species, loss of PSII core proteins, and cell death. Our study lays foundations for optimising photomixotrophy-based biotechnologies through understanding the coordination of the crosstalk between photosynthetic electron transport and metabolism.
(© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.)
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معلومات مُعتمدة: BIO-284 Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía; European Molecular Biology Organization; NNF20OC0064371 Novo Nordisk Fonden; FPU18/06580 Ministerio de Universidades; PID2019-104513GB-I00 Ministerio de Ciencia, Innovación y Universidades; 82845 NordForsk
فهرسة مساهمة: Keywords: cyanobacteria; glucose; glycogen; metabolism; mixotrophy; photomixotrophy; photosynthesis
المشرفين على المادة: 9005-79-2 (Glycogen)
0 (Photosystem II Protein Complex)
IY9XDZ35W2 (Glucose)
142M471B3J (Carbon Dioxide)
S88TT14065 (Oxygen)
EC 2.7.7.27 (Glucose-1-Phosphate Adenylyltransferase)
EC 5.4.2.2 (Phosphoglucomutase)
تواريخ الأحداث: Date Created: 20240506 Date Completed: 20240606 Latest Revision: 20240606
رمز التحديث: 20240606
DOI: 10.1111/nph.19793
PMID: 38706429
قاعدة البيانات: MEDLINE
الوصف
تدمد:1469-8137
DOI:10.1111/nph.19793