دورية أكاديمية
Insights into spheroids formation in cellulose nanofibrils and Matrigel hydrogels using AFM-based techniques.
| العنوان: | Insights into spheroids formation in cellulose nanofibrils and Matrigel hydrogels using AFM-based techniques. |
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| المؤلفون: | Teixeira Polez R; Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, FI-00076, Aalto, Finland.; Drug Research Program, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, 00790, Helsinki, Finland., Huynh N; Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, FI-00076, Aalto, Finland., Pridgeon CS; Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, FI-00076, Aalto, Finland.; Drug Research Program, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, 00790, Helsinki, Finland., Valle-Delgado JJ; Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, FI-00076, Aalto, Finland., Harjumäki R; Drug Research Program, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, 00790, Helsinki, Finland., Österberg M; Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, FI-00076, Aalto, Finland. |
| المصدر: | Materials today. Bio [Mater Today Bio] 2024 Apr 17; Vol. 26, pp. 101065. Date of Electronic Publication: 2024 Apr 17 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Ltd Country of Publication: England NLM ID: 101757228 Publication Model: eCollection Cited Medium: Internet ISSN: 2590-0064 (Electronic) Linking ISSN: 25900064 NLM ISO Abbreviation: Mater Today Bio Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : Elsevier Ltd., [2019]- |
| مستخلص: | The recent FDA decision to eliminate animal testing requirements emphasises the role of cell models, such as spheroids, as regulatory test alternatives for investigations of cellular behaviour, drug responses, and disease modelling. The influence of environment on spheroid formation are incompletely understood, leading to uncertainty in matrix selection for scaffold-based 3D culture. This study uses atomic force microscopy-based techniques to quantify cell adhesion to Matrigel and cellulose nanofibrils (CNF), and cell-cell adhesion forces, and their role in spheroid formation of hepatocellular carcinoma (HepG2) and induced pluripotent stem cells (iPS(IMR90)-4). Results showed different cell behaviour in CNF and Matrigel cultures. Both cell lines formed compact spheroids in CNF but loose cell aggregates in Matrigel. Interestingly, the type of cell adhesion protein, and not the bond strength, appeared to be a key factor in the formation of compact spheroids. The gene expression of E- and N-cadherins, proteins on cell membrane responsible for cell-cell interactions, was increased in CNF culture, leading to formation of compact spheroids while Matrigel culture induced integrin-laminin binding and downregulated E-cadherin expression, resulting in looser cell aggregates. These findings enhance our understanding of cell-biomaterial interactions in 3D cultures and offer insights for improved 3D cell models, culture biomaterials, and applications in drug research. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors.) |
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| فهرسة مساهمة: | Keywords: Atomic force microscopy; Cell adhesion molecules; Cell interactions; Cellulose nanofibrils; Matrigel; Spheroid formation |
| تواريخ الأحداث: | Date Created: 20240506 Latest Revision: 20240507 |
| رمز التحديث: | 20240507 |
| مُعرف محوري في PubMed: | PMC11066555 |
| DOI: | 10.1016/j.mtbio.2024.101065 |
| PMID: | 38706731 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2590-0064 |
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| DOI: | 10.1016/j.mtbio.2024.101065 |