| المؤلفون: |
Paz MGOD; Department of Microbiology-Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., Bezerra MF; Department of Microbiology-Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., Mendes AF; Department of Microbiology-Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., da Silva A; Department of Microbiology-Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., Lima GB; Department of Microbiology-Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., Gil LHVG; Department of Virology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., Pena LJ; Department of Virology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., Diniz GTN; Statistics and Geoprocessing Center, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., Drexler JF; Institute of Virology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin, Germany., Oriá RB; Laboratory of the Biology of Tissue Healing, Ontogeny and Nutrition, Department of Morphology, Institute of Biomedicine, School of Medicine, Federal University of Ceará, Fortaleza, Brazil., Ferreira de Oliveira-Filho E; Institute of Virology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin, Germany., Reis CRS; Department of Microbiology-Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil. |
| مستخلص: |
Hepatitis E virus (HEV) infects roughly 20 million people worldwide, causing self-limiting acute hepatic disease that can evolve into a chronic course. HEV-3, HEV-4, and HEV-7 genotypes are zoonotic and transmitted to humans by consuming raw or undercooked meat. Here, we developed an indirect ELISA based on the recombinant HEV-3 capsid and performed a seroprevalence study on domestic swine in northeastern Brazil. Our in-house ELISA was initially validated using a subset of 79 sera characterized by concordant results for two distinct commercial ELISA kits. Our ELISA exhibited excellent sensitivity (94%) and specificity (100%), with an area under the curve of 0.99 Further testing, including 212 swine sera, revealed a seroprevalence of 57.5% (95% confidence interval, 50.6-64.3%). Our findings indicate that the novel ELISA test could accurately detect specific anti-HEV antibodies in domestic pigs and should be further validated in humans and other mammals. |
