دورية أكاديمية
The beta pore-forming bacterial pesticidal protein Tpp78Aa1 is toxic to the Asian citrus psyllid vector of the citrus greening bacterium.
| العنوان: | The beta pore-forming bacterial pesticidal protein Tpp78Aa1 is toxic to the Asian citrus psyllid vector of the citrus greening bacterium. |
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| المؤلفون: | Tavares CS; Department of Entomology and Nematology, University of Florida, Gainesville, FL, USA. Electronic address: clebsondossantos@ufl.edu., Mishra R; Department of Entomology and Nematology, University of Florida, Gainesville, FL, USA., Kishk A; Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, FL 33850, USA; Department of Plant Protection, Faculty of Agriculture, Tanta University 31527, Egypt., Wang X; Department of Entomology and Nematology, University of Florida, Gainesville, FL, USA., Ghobrial PN; Department of Entomology and Nematology, University of Florida, Gainesville, FL, USA., Killiny N; Department of Plant Protection, Faculty of Agriculture, Tanta University 31527, Egypt., Bonning BC; Department of Entomology and Nematology, University of Florida, Gainesville, FL, USA. |
| المصدر: | Journal of invertebrate pathology [J Invertebr Pathol] 2024 Jun; Vol. 204, pp. 108122. Date of Electronic Publication: 2024 May 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0014067 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0805 (Electronic) Linking ISSN: 00222011 NLM ISO Abbreviation: J Invertebr Pathol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Hemiptera*/microbiology , Bacterial Proteins* , Pest Control, Biological*, Animals ; Citrus/microbiology ; Insect Vectors ; Bacillus thuringiensis/chemistry ; Plant Diseases/microbiology ; Insecticides |
| مستخلص: | The Asian citrus psyllid (ACP) Diaphorina citri transmits the causative agent of huanglongbing, or citrus greening disease, that has decimated global citrus production. Pesticidal proteins derived from bacteria such as Bacillus thuringiensis (Bt) can provide effective and environmentally friendly alternatives for management of D. citri, but few with sufficient toxicity to D. citri have been identified. Here, we report on the toxicity of 14 Bt-derived pesticidal proteins from five different structural groups against D. citri. These proteins were selected based on previously reported toxicity to other hemipteran species and on pesticidal protein availability. Most of the proteins were expressed in Escherichia coli and purified from inclusion bodies or His-tag affinity purification, while App6Aa2 was expressed in Bt and purified from spore/crystal mixtures. Pesticidal proteins were initially screened by feeding psyllids on a single dose, and lethal concentration (LC Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: [BCB, CT and RM are authors on a provisional patent application associated with this work. AK, XW, PNG and NK declare no competing interests.]. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bacillus thuringiensis; Diaphorina citri; Huanglongbing; Pesticidal proteins; Tpp78Aa1 |
| SCR Organism: | Diaphorina citri |
| تواريخ الأحداث: | Date Created: 20240506 Date Completed: 20240521 Latest Revision: 20240802 |
| رمز التحديث: | 20240803 |
| DOI: | 10.1016/j.jip.2024.108122 |
| PMID: | 38710321 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0805 |
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| DOI: | 10.1016/j.jip.2024.108122 |