التفاصيل البيبلوغرافية
| العنوان: |
Cytochrome GmGLY1 is Involved in the Biosynthesis of Glycitein in Soybean. |
| المؤلفون: |
Zhang P; National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China.; Zhejiang Lab, Hangzhou 311121, China., Yang C; National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China., Wang J; National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China., Jiang P; National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China., Qi J; National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China., Hou W; National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China., Cheng H; National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China., Feng X; Zhejiang Lab, Hangzhou 311121, China., Yu D; National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China. |
| المصدر: |
Journal of agricultural and food chemistry [J Agric Food Chem] 2024 May 15; Vol. 72 (19), pp. 10944-10957. Date of Electronic Publication: 2024 May 06. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: |
Glycine max*/metabolism , Glycine max*/genetics , Glycine max*/chemistry , Isoflavones*/metabolism , Isoflavones*/biosynthesis , Plant Proteins*/genetics , Plant Proteins*/metabolism , Polymorphism, Single Nucleotide* , Seeds*/metabolism , Seeds*/genetics , Seeds*/chemistry , Genome-Wide Association Study*, Cytochrome P-450 Enzyme System/genetics ; Cytochrome P-450 Enzyme System/metabolism ; Gene Expression Regulation, Plant |
| مستخلص: |
Isoflavones, the major secondary metabolites of interest due to their benefits to both human and plant health, are exclusively produced by legumes. In this study, we profiled the isoflavone content in dry seeds from 211 soybean [ Glycine max (L.) Merr.] accessions grown across five environments. Broad and discernible phenotypic variations were observed among accessions, regions, and years of growth. Twenty-six single-nucleotide polymorphisms (SNPs) associated with the sum of glycitein (GLE), glycitin (GL), 6″-O-acetylglycitin (AGL), and 6″-O-malonylglycitin (MGL) contents were detected in multiple environments via a genome-wide association study (GWAS). These SNPs were located on chromosome 11 (8,148,438 bp to 8,296,956 bp, renamed qGly11-01 ). Glyma.11g108300 ( GmGLY1 ), a gene that encodes a P450 family protein, was identified via sequence variation analysis, functional annotation, weighted gene coexpression network analysis (WGCNA), and expression profile analysis of candidate gene, and hairy roots transformation in soybean. Overexpression of GmGLY1 increased the glycitein content (GLC) in soybean hairy roots and transgenic seeds, while CRISPR/Cas9-generated mutants exhibited decreased GLC and increased daidzein content (DAC). Haplotype analysis revealed that GmGLY1 allelic variations significantly affect the GLC accumulation. These findings enhance our understanding of genes influencing GLC in soybean and may guide breeding for lines with high and stable GLC. |
| فهرسة مساهمة: |
Keywords: Glycine max (L.) Merr.; GmGLY1; cytochrome p450; gene cloning; genome-wide association study; glycitein biosynthesis; isoflavones; secondary metabolites; soybean |
| تواريخ الأحداث: |
Date Created: 20240506 Date Completed: 20240515 Latest Revision: 20240603 |
| رمز التحديث: |
20240604 |
| DOI: |
10.1021/acs.jafc.4c00968 |
| PMID: |
38710505 |
| قاعدة البيانات: |
MEDLINE |
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