| المؤلفون: |
Nie J; College of Agriculture, Guangxi University; Guangxi Key Laboratory of Agro-environment and Agric-products Safety; Key Laboratory of Crop Genetic Breeding and Germplasm Innovation, Nanning 530004, PR China., Ma W; College of Agriculture, Guangxi University; Guangxi Key Laboratory of Agro-environment and Agric-products Safety; Key Laboratory of Crop Genetic Breeding and Germplasm Innovation, Nanning 530004, PR China., Ma X; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China., Zhu; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China., Li X; College of Agriculture, Guangxi University; Guangxi Key Laboratory of Agro-environment and Agric-products Safety; Key Laboratory of Crop Genetic Breeding and Germplasm Innovation, Nanning 530004, PR China., Wang C; College of Agriculture, Guangxi University; Guangxi Key Laboratory of Agro-environment and Agric-products Safety; Key Laboratory of Crop Genetic Breeding and Germplasm Innovation, Nanning 530004, PR China., Xu G; College of Agriculture, Guangxi University; Guangxi Key Laboratory of Agro-environment and Agric-products Safety; Key Laboratory of Crop Genetic Breeding and Germplasm Innovation, Nanning 530004, PR China., Chen C; College of Agriculture, Guangxi University; Guangxi Key Laboratory of Agro-environment and Agric-products Safety; Key Laboratory of Crop Genetic Breeding and Germplasm Innovation, Nanning 530004, PR China., Luo D; College of Agriculture, Guangxi University; Guangxi Key Laboratory of Agro-environment and Agric-products Safety; Key Laboratory of Crop Genetic Breeding and Germplasm Innovation, Nanning 530004, PR China., Xie S; College of Agriculture, Guangxi University; Guangxi Key Laboratory of Agro-environment and Agric-products Safety; Key Laboratory of Crop Genetic Breeding and Germplasm Innovation, Nanning 530004, PR China., Hu G; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China., Chen P; College of Agriculture, Guangxi University; Guangxi Key Laboratory of Agro-environment and Agric-products Safety; Key Laboratory of Crop Genetic Breeding and Germplasm Innovation, Nanning 530004, PR China. |
| مستخلص: |
Bama County is a world-famous longevity county in the Guangxi Province, China. Bama hemp is a traditional seed used in hemp cultivation in the Bama County. The seeds contain abundant unsaturated fatty acids, particularly linoleic acid (LA) and linolenic acid in the golden ratio. These two substances have been proven to be related to human health and the prevention of various diseases. However, the seed development and seed oil accumulation mechanisms remain unclear. This study employed a combined analysis of physiological, transcriptomic, and metabolomic parameters to elucidate the fatty acid formation patterns in Bama hemp seeds throughout development. We found that seed oil accumulated at a late stage in embryo development, with seed oil accumulation following an "S″-shaped growth curve, and positively correlated with seed size, sugar content, protein content, and starch content. Transcriptome analysis identified genes related to the metabolism of LA, α-linolenic acid (ALA), and jasmonic acid (JA). We found that the FAD2 gene was upregulated 165.26 folds and the FAD3 gene was downregulated 6.15 folds at day 21. Metabolomic changes in LA, ALA, and JA compounds suggested a competitive relationship among these substances. Our findings indicate that the peak period of substance accumulation and nutrient accumulation in Bama hemp seeds occurs during the midstage of seed development (day 21) rather than in the late stage (day 40). The results of this research will provide a theoretical basis for local cultivation and deep processing of Bama hemp. |
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