دورية أكاديمية
Translational control of MPS1 links protein synthesis with the initiation of cell division and spindle pole body duplication in Saccharomyces cerevisiae.
| العنوان: | Translational control of MPS1 links protein synthesis with the initiation of cell division and spindle pole body duplication in Saccharomyces cerevisiae. |
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| المؤلفون: | Blank HM; Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX 77843, USA., Alonso A; Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA., Fabritius AS; Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA., Valk E; Institute of Technology, University of Tartu, Tartu 50411, Estonia., Loog M; Institute of Technology, University of Tartu, Tartu 50411, Estonia., Winey M; Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA., Polymenis M; Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX 77843, USA. |
| المصدر: | Genetics [Genetics] 2024 Jul 08; Vol. 227 (3). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0374636 Publication Model: Print Cited Medium: Internet ISSN: 1943-2631 (Electronic) Linking ISSN: 00166731 NLM ISO Abbreviation: Genetics Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [Oxford] : Oxford University Press Original Publication: Austin, Tex. [etc.] |
| مواضيع طبية MeSH: | Saccharomyces cerevisiae Proteins*/genetics , Saccharomyces cerevisiae Proteins*/metabolism , Saccharomyces cerevisiae*/genetics , Saccharomyces cerevisiae*/metabolism , Spindle Pole Bodies*/metabolism , Spindle Pole Bodies*/genetics, Cyclins/metabolism ; Cyclins/genetics ; Protein Serine-Threonine Kinases/genetics ; Protein Serine-Threonine Kinases/metabolism ; Open Reading Frames ; Protein Biosynthesis ; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; Cell Division/genetics ; Repressor Proteins/genetics ; Repressor Proteins/metabolism ; Gene Expression Regulation, Fungal |
| مستخلص: | Protein synthesis underpins cell growth and controls when cells commit to a new round of cell division at a point in late G1 of the cell cycle called Start. Passage through Start also coincides with the duplication of the microtubule-organizing centers, the yeast spindle pole bodies, which will form the 2 poles of the mitotic spindle that segregates the chromosomes in mitosis. The conserved Mps1p kinase governs the duplication of the spindle pole body (SPB) in Saccharomyces cerevisiae. Here, we show that the MPS1 transcript has a short upstream open reading frame (uORF) that represses the synthesis of Mps1p. Mutating the MPS1 uORF makes the cells smaller, accelerates the appearance of Mps1p in late G1, and promotes completion of Start. Monitoring the SPB in the cell cycle using structured illumination microscopy revealed that mutating the MPS1 uORF enabled cells to duplicate their SPB earlier at a smaller cell size. The accelerated Start of MPS1 uORF mutants depends on the G1 cyclin Cln3p and the transcriptional repressor Whi5p but not on the Cln1,2p G1 cyclins. These results identify growth inputs in mechanisms that control duplication of the microtubule-organizing center and implicate these processes in the coupling of cell growth with division. Competing Interests: Conflicts of interest The author(s) declare no conflict of interest. (© The Author(s) 2024. Published by Oxford University Press on behalf of The Genetics Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.) |
| معلومات مُعتمدة: | R01 GM123139 United States GM NIGMS NIH HHS; R01 GM123139 United States GF NIH HHS; T32 CA108459 United States CA NCI NIH HHS |
| فهرسة مساهمة: | Keywords: MTOC; SIM; SPB; cell size; start |
| المشرفين على المادة: | 0 (Saccharomyces cerevisiae Proteins) EC 2.7.12.2 (MPS1 protein, S cerevisiae) 0 (Cyclins) 0 (Whi5 protein, S cerevisiae) EC 2.7.11.1 (Protein Serine-Threonine Kinases) 0 (CLN3 protein, S cerevisiae) 0 (Cell Cycle Proteins) 0 (Repressor Proteins) |
| تواريخ الأحداث: | Date Created: 20240507 Date Completed: 20240708 Latest Revision: 20240801 |
| رمز التحديث: | 20240801 |
| DOI: | 10.1093/genetics/iyae069 |
| PMID: | 38713088 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1943-2631 |
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| DOI: | 10.1093/genetics/iyae069 |