دورية أكاديمية
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Study of individual domains contributing to MALT1 dimerization in BCL10-independent and dependent assembly.

التفاصيل البيبلوغرافية
العنوان: Study of individual domains contributing to MALT1 dimerization in BCL10-independent and dependent assembly.
المؤلفون: Kuo BJ; Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan., Lin SC; Genomics Research Center, Academia Sinica, Taipei, Taiwan., Tu YF; Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan; Genomics Research Center, Academia Sinica, Taipei, Taiwan., Huang PH; Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan., Lo YC; Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan. Electronic address: gracelo@ncku.edu.tw.
المصدر: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Jul 12; Vol. 717, pp. 150029. Date of Electronic Publication: 2024 Apr 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
أسماء مطبوعة: Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press.
مواضيع طبية MeSH: Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein*/metabolism , Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein*/chemistry , Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein*/genetics , B-Cell CLL-Lymphoma 10 Protein*/metabolism , B-Cell CLL-Lymphoma 10 Protein*/chemistry , B-Cell CLL-Lymphoma 10 Protein*/genetics , Protein Multimerization* , Protein Domains*, Humans ; Crystallography, X-Ray ; Models, Molecular ; Neoplasm Proteins/metabolism ; Neoplasm Proteins/chemistry ; Neoplasm Proteins/genetics ; Caspases/metabolism ; Caspases/chemistry
مستخلص: The CARMA-BCL10-MALT1 (CBM) signalosome functions as a pivotal supramolecular module, integrating diverse receptor-induced signaling pathways to regulate BCL10-dependent NF-kB activation in innate and adaptive immunity. Conversely, the API2-MALT1 fusion protein in t(11; 18)(q21; q21) MALT lymphoma constitutively induces BCL10-independent NF-kB activation. MALT1 dimer formation is indispensable for the requisite proteolytic activity and is critical for NF-kB activation regulation in both scenarios. However, the molecular assembly of MALT1 individual domains in CBM activation remains elusive. Here we report the crystal structure of the MALT1 death domain (DD) at a resolution of 2.1 Å, incorporating reconstructed residues in previously disordered loops 1 and 2. Additionally, we observe a conformational regulation element (CRE) regulating stem-helix formation in NLRPs pyrin (PYD) within the MALT1 DD structure. The structure reveals a stem-helix-mediated dimer further corroborated in solution. To elucidate how the BCL10 filament facilitates MALT1 dimerization, we reconstitute a BCL10-CARD-MALT1-DD-IG1-IG2 complex model. We propose a N+7 rule for BCL10-dependent MALT1 dimerization via the IG1-IG2 domain and for MALT1-dependent cleavage in trans. Biochemical data further indicates concentration-dependent dimerization of the MALT1 IG1-IG2 domain, facilitating MALT1 dimerization in BCL10-independent manner. Our findings provide a structural and biochemical foundation for understanding MALT1 dimeric mechanisms, shedding light on potential BCL10-independent MALT1 dimer formation and high-order BCL10-MALT1 assembly.
Competing Interests: Declaration of competing interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: CBM signalosome; Crystal structure; Death domain; MALT lymphoma; MALT1 dimerization; Stem-helix dimer
المشرفين على المادة: 0 (MALT1 protein, human)
0 (BCL10 protein, human)
تواريخ الأحداث: Date Created: 20240507 Date Completed: 20240519 Latest Revision: 20240528
رمز التحديث: 20240528
DOI: 10.1016/j.bbrc.2024.150029
PMID: 38714015
قاعدة البيانات: MEDLINE
الوصف
تدمد:1090-2104
DOI:10.1016/j.bbrc.2024.150029