دورية أكاديمية
Salivary Gland Tissue Recombination Can Modify Cell Fate.
| العنوان: | Salivary Gland Tissue Recombination Can Modify Cell Fate. |
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| المؤلفون: | Sekiguchi R; Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA., Martin D; Genomics and Computational Biology Core, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA., Doyle AD; Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.; Imaging Core, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA., Wang S; Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Yamada KM; Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA. |
| مؤلفون مشاركون: | Genomics and Computational Biology Core; National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD, USA. |
| المصدر: | Journal of dental research [J Dent Res] 2024 Jul; Vol. 103 (7), pp. 755-764. Date of Electronic Publication: 2024 May 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Sage Country of Publication: United States NLM ID: 0354343 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1544-0591 (Electronic) Linking ISSN: 00220345 NLM ISO Abbreviation: J Dent Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Thousand Oaks, CA : Sage Original Publication: Chicago, American Dental Assn. [etc.] |
| مواضيع طبية MeSH: | Cell Differentiation* , Submandibular Gland*/embryology , Submandibular Gland*/cytology , Mesoderm*/cytology , Mesoderm*/embryology, Animals ; Mice ; Parotid Gland/cytology ; Parotid Gland/embryology ; Parotid Gland/metabolism ; Epithelial Cells ; Salivary Glands/embryology ; Salivary Glands/cytology ; Cell Lineage ; Acinar Cells ; Epithelium/embryology |
| مستخلص: | Although mesenchyme is essential for inducing the epithelium of ectodermal organs, its precise role in organ-specific epithelial fate determination remains poorly understood. To elucidate the roles of tissue interactions in cellular differentiation, we performed single-cell RNA sequencing and imaging analyses on recombined tissues, where mesenchyme and epithelium were switched ex vivo between two types of embryonic mouse salivary glands: the parotid gland (a serous gland) and the submandibular gland (a predominantly mucous gland). We found partial induction of molecules that define gland-specific acinar and myoepithelial cells in recombined salivary epithelium. The parotid epithelium recombined with submandibular mesenchyme began to express mucous acinar genes not intrinsic to the parotid gland. While myoepithelial cells do not normally line parotid acini, newly induced myoepithelial cells densely populated recombined parotid acini. However, mucous acinar and myoepithelial markers continued to be expressed in submandibular epithelial cells recombined with parotid mesenchyme. Consequently, some epithelial cells appeared to be plastic, such that their fate could still be modified in response to mesenchymal signaling, whereas other epithelial cells appeared to be already committed to a specific fate. We also discovered evidence for bidirectional induction: transcriptional changes were observed not only in the epithelium but also in the mesenchyme after heterotypic tissue recombination. For example, parotid epithelium induced the expression of muscle-related genes in submandibular fibroblasts that began to mimic parotid fibroblast gene expression. These studies provide the first comprehensive unbiased molecular characterization of tissue recombination approaches exploring the regulation of cell fate. Competing Interests: Declaration of Conflicting InterestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. |
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| معلومات مُعتمدة: | Z01 DE000525 United States ImNIH Intramural NIH HHS; R01 DC000086 United States DC NIDCD NIH HHS; ZIC DC000086 United States ImNIH Intramural NIH HHS; ZIG DE000740 United States ImNIH Intramural NIH HHS; ZIA DE000525 United States ImNIH Intramural NIH HHS; ZIC DE000750 United States ImNIH Intramural NIH HHS |
| فهرسة مساهمة: | Keywords: developmental biology; epithelial-mesenchymal interaction; gene expression; morphogenesis; salivary physiology; single cell RNAseq |
| تواريخ الأحداث: | Date Created: 20240508 Date Completed: 20240620 Latest Revision: 20240623 |
| رمز التحديث: | 20240623 |
| مُعرف محوري في PubMed: | PMC11191754 |
| DOI: | 10.1177/00220345241247484 |
| PMID: | 38715201 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1544-0591 |
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| DOI: | 10.1177/00220345241247484 |