دورية أكاديمية
[Analysis of early changes in lymphocyte subpopulations after liver transplantation and their correlation with clinical manifestations].
| العنوان: | [Analysis of early changes in lymphocyte subpopulations after liver transplantation and their correlation with clinical manifestations]. |
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| المؤلفون: | Li WQ; Graduate School, Guangzhou University of Chinese Medicine, Guangzhou 510006, China Department of Laboratory Medicine, General Hospital of Southern Theater Command, Guangzhou 510010, China., Zhang WY; Department of Laboratory Medicine, General Hospital of Southern Theater Command, Guangzhou 510010, China The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China., Li X; Department of Laboratory Medicine, General Hospital of Southern Theater Command, Guangzhou 510010, China., Zhang YM; Department of Laboratory Medicine, General Hospital of Southern Theater Command, Guangzhou 510010, China The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China., Sun ZH; Graduate School, Guangzhou University of Chinese Medicine, Guangzhou 510006, China Department of Laboratory Medicine, General Hospital of Southern Theater Command, Guangzhou 510010, China The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China. |
| المصدر: | Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] [Zhonghua Yu Fang Yi Xue Za Zhi] 2024 May 06; Vol. 58 (5), pp. 679-685. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhonghua yi xue hui Country of Publication: China NLM ID: 7904962 Publication Model: Print Cited Medium: Print ISSN: 0253-9624 (Print) Linking ISSN: 02539624 NLM ISO Abbreviation: Zhonghua Yu Fang Yi Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Zhonghua yi xue hui Original Publication: Beijing : Zhonghua yi xue hui [1967?]- |
| مواضيع طبية MeSH: | Liver Transplantation* , Lymphocyte Subsets*/immunology, Humans ; Retrospective Studies ; Postoperative Period ; Lymphocyte Count ; Male ; Female ; Graft Rejection/immunology |
| مستخلص: | This study aimed to investigate the differences in peripheral blood lymphocyte subsets among patients with different immune statuses in the early postoperative period after liver transplantation, as well as the dynamic changes during the early post-transplantation period. A retrospective study was conducted, selecting a total of 82 patients who underwent liver transplantation at the General Hospital of PLA Southern Theater Command from January, 2018 to December, 2023. Based on the patients' postoperative immune status, they were categorized into stable group ( n =40), infection group ( n =21), and rejection group ( n =21). Peripheral blood samples of 2-3 ml were collected from patients at weeks 1 to 4 postoperatively, and flow cytometry was employed to measure the absolute values of peripheral blood lymphocyte subsets. For metric data conforming to normal distribution and homogeneity of variance, multiple group comparisons were conducted using ANOVA and Bonferroni multiple comparisons; for non-normally distributed data, the Kruskal Wallis test was used. Friedman test was used to compare different time periods within 4 weeks after liver transplantation. The results showed that there were no statistically significant differences in the absolute values of lymphocyte subsets among the three groups in the first week after liver transplantation ( P >0.05); however, significant differences were observed in the absolute values of lymphocyte subsets among the three groups in the second, third, and fourth weeks postoperatively ( P <0.05). In the second week, the rejection group showed significantly higher absolute counts of T cells, CD4 + T cells, CD8 + T cells, NK cells, and B cells compared to the infection group (585.0 vs. 199.0; 324.0 vs. 113.0; 188.0 vs. 56.0; 57.0 vs. 11.0; 145.0 vs. 65.0 cells/μl), with statistically significant differences ( Z =-3.972, P <0.001; Z =-3.590, P =0.001; Z =-3.978, P <0.001; Z =-3.072, P =0.006; Z =-2.472, P =0.040). In the third week, the rejection group showed significantly higher absolute counts of T cells, CD4 + T cells, and CD8 + T cells compared to the infection group (660.0 vs. 216.0; 350.0 vs. 123.0; 184.0 vs. 76.0 cells/μl), with statistically significant differences ( Z =-3.019, P =0.008; Z =-3.492, P =0.001; Z =-2.845, P =0.013). In the fourth week, the rejection group showed significantly higher absolute counts of T cells, CD4 + T cells, CD8 + T cells, and B cells compared to the infection group (690.0 vs. 273.0; 405.0 vs. 168.0; 214.0 vs. 96.0; 117.0 vs. 48.0 cells/μl), with statistically significant differences ( Z =-3.379, P =0.002; Z =-3.068, P =0.006; Z =-3.007, P =0.0086; Z =-2.330, P =0.020). Within 4 weeks after liver transplantation, the absolute values of T cells, CD8 + T cells, and NK cells in the fourth week were higher than those in the first week, with statistically significant differences ( Z =-3.825, P =0.001; Z =-3.466, P =0.003; Z =-3.526, P =0.003); however, the absolute values of B cells showed an overall decreasing trend, and were significantly lower in the fourth week than in the first and second weeks, with statistically significant differences ( Z =3.705, P =0.001; Z =2.630, P =0.009). The changes in lymphocyte subset absolute values in the rejection group were more significant than those in the infection group, with T cells, CD4 + T cells, and CD8 + T cells showing significant increases in the second, third, and fourth weeks postoperatively compared with the first week, with statistically significant differences ( Z =-3.466, P =0.003; Z =-4.661, P <0.001; Z =-5.020, P <0.001; Z =-2.749, P =0.036; Z =-4.422, P <0.001; Z =-4.542, P <0.001; Z =-3.466, P =0.003; Z =-3.765, P =0.001; Z =-4.482, P <0.001); NK cell absolute values in the third and fourth weeks postoperatively were significantly higher than those in the first week, with statistically significant differences ( Z =-2.570, P =0.061; Z =-3.765, P =0.001). In summary, monitoring the differences and dynamic changes of lymphocyte subsets in patients after liver transplantation may have certain guiding significance for evaluating the immune function status of patients and adjusting treatment plans. |
| معلومات مُعتمدة: | 202002030078 Guangzhou Science and Technology Plan Project; B2022095 Guangdong Provincial Medical Science and Technology Research Fund Project |
| فهرسة مساهمة: | Local Abstract: [Publisher, Chinese] 探讨外周血淋巴细胞亚群在肝移植术后早期处于不同免疫状态患者中的差异性及移植术后早期的动态变化。采用回顾性研究方法，选取2018年1月至2023年12月于中国人民解放军南部战区总医院接受肝移植手术患者共82例作为研究对象。根据患者术后免疫状态，将患者分为稳定组（ n =40）、感染组（ n =21）和排斥组（ n =21）。术后第1周、第2周、第3周、第4周采集患者静脉血2~3 ml，采用流式细胞仪检测患者外周血淋巴细胞亚群绝对值。符合正态分布且方差齐的计量资料，多组间比较使用ANOVA检验及Bonferroni多重比较；非正态分布的数据采用Kruskal-Wallis检验；肝移植术后4周内不同时间段的比较采用Friedman检验。结果显示，肝移植术后第1周三组之间各淋巴细胞亚群绝对值差异无统计学意义（ P >0.05）；术后第2周、第3周、第4周三组之间淋巴细胞亚群绝对值差异有统计学意义（ P <0.05）。第2周排斥组与感染组比较，T细胞、CD4 + T细胞、CD8 + T细胞、NK细胞、B细胞绝对值计数明显升高（585.0 vs. 199.0；324.0 vs .113.0；188.0 vs .56.0；57.0 vs .11.0；145.0 vs .65.0 个/μl），差异有统计学意义（ Z =-3.972， P <0.001； Z =-3.590， P =0.001； Z =-3.978， P <0.001； Z =-3.072， P =0.006； Z =-2.472， P =0.040）。第3周排斥组与感染组比较，T细胞、CD4 + T细胞、CD8 + T细胞绝对值计数明显升高（660.0 vs . 216.0；350.0 vs . 123.0；184.0 vs .76.0 个/μl），差异有统计学意义（ Z =-3.019， P =0.008； Z =-3.492， P= 0.001； Z =-2.845， P= 0.013）。第4周排斥组与感染组比较，T细胞、CD4 + T细胞、CD8 + T细胞、B细胞绝对值计数明显升高（690.0 vs .273.0；405.0 vs .168.0；214.0 vs .96.0；117.0 vs .48.0 个/μl），差异有统计学意义（ Z =-3.379， P =0.002； Z =-3.068， P= 0.006； Z =-3.007， P= 0.0086； Z =-2.330， P= 0.020）。肝移植术后4周内，T细胞、CD8 + T细胞、NK细胞绝对值术后第4周高于第1周，差异有统计学意义（ Z =-3.825， P =0.001； Z =-3.466， P =0.003； Z =-3.526， P =0.003）；B细胞绝对值总体呈下降趋势，术后第4周显著低于第1周、第2周，差异有统计学意义（ Z =3.705， P =0.001； Z =2.630， P =0.009）。排斥组淋巴细胞亚群绝对值变化较感染组更为显著，T细胞、CD4 + T细胞、CD8 + T细胞绝对值术后第2、3、4周显著高于第1周，差异有统计学意义（ Z =-3.466， P =0.003； Z =-4.661， P <0.001； Z =-5.020， P <0.001； Z =-2.749， P =0.036； Z =-4.422， P <0.001； Z =-4.542， P <0.001； Z =-3.466， P =0.003； Z =-3.765， P =0.001； Z =-4.482， P <0.001）；NK细胞绝对值术后第3、4周显著高于第1周，差异有统计学意义（ Z =-2.570， P =0.061； Z =-3.765， P =0.001）。综上，监测肝移植术后患者淋巴细胞亚群的差异性及动态性变化，可能对评估患者免疫功能状态及调整治疗方案具有一定指导意义。. |
| تواريخ الأحداث: | Date Created: 20240508 Date Completed: 20240508 Latest Revision: 20240508 |
| رمز التحديث: | 20240508 |
| DOI: | 10.3760/cma.j.cn112150-20231120-00357 |
| PMID: | 38715509 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0253-9624 |
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| DOI: | 10.3760/cma.j.cn112150-20231120-00357 |