دورية أكاديمية
أعرض في EDS

Development and evaluation of time-resolved rapid immunofluorescence test for detection of TSOL18 specific antibody in porcine cysticercosis infections.

التفاصيل البيبلوغرافية
العنوان: Development and evaluation of time-resolved rapid immunofluorescence test for detection of TSOL18 specific antibody in porcine cysticercosis infections.
المؤلفون: Zhang D; College of Animal Science and Technology, Inner Mongolia MinZu University, Tongliao, 028000, China., Duan R; College of Animal Science and Technology, Inner Mongolia MinZu University, Tongliao, 028000, China., Liu J; Qingdao Special Servicemen Recuperation Center of PLA Navy, Qingdao, 266071, China., Wang M; College of Animal Science and Technology, Inner Mongolia MinZu University, Tongliao, 028000, China., Yang Y; College of Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, China., Zhao Y; College of Animal Science and Technology, Inner Mongolia MinZu University, Tongliao, 028000, China., Liu M; State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, 130062, China. liumy36@163.com.; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225000, China. liumy36@163.com., Sun S; College of Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, China. shums1975@163.com.
المصدر: BMC veterinary research [BMC Vet Res] 2024 May 08; Vol. 20 (1), pp. 182. Date of Electronic Publication: 2024 May 08.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2005-
مواضيع طبية MeSH: Swine Diseases*/diagnosis , Swine Diseases*/parasitology , Swine Diseases*/blood , Cysticercosis*/veterinary , Cysticercosis*/diagnosis , Sensitivity and Specificity* , Antibodies, Helminth*/blood , Antigens, Helminth*/blood , Antigens, Helminth*/immunology , Fluorescent Antibody Technique*/veterinary , Fluorescent Antibody Technique*/methods , Enzyme-Linked Immunosorbent Assay*/veterinary , Enzyme-Linked Immunosorbent Assay*/methods, Animals ; Swine ; Cysticercus/immunology ; Taenia solium/immunology
مستخلص: Background: Porcine cysticercosis, a serious zoonotic parasitic disease, is caused by the larvae of Taenia solium and has been acknowledged by the World Organization for Animal Health. The current detection methods of Cysticercus cellulosae cannot meet the needs of large-scale and rapid detection in the field. We hypothesized that the immunofluorescence chromatography test strip (ICS) for detecting Cysticercus cellulosae, according to optimization of a series of reaction systems was conducted, and sensitivity, specificity, and stability testing, and was finally compared with ELISA. This method utilizes Eu 3+ -labeled time-resolved fluorescent microspheres (TRFM) coupled with TSOL18 antigen to detect TSOL18 antibodies in infected pig sera.
Results: ICS and autopsy have highly consistent diagnostic results (n = 133), as determined by Cohen's κ analysis (κ = 0.925). And the results showed that the proposed ICS are high sensitivity (0.9459) with specificity (0.9792). The ICS was unable to detect positive samples of other parasites. It can be stored for at least six months at 4℃.
Conclusions: In summary, we established a TRFM-ICS method with higher sensitivity and specificity than indirect ELISA. Results obtained from serum samples can be read within 10 min, indicating a rapid, user-friendly test suitable for large-scale field detection.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 2019LH03021 the Natural Science Foundation of Inner Mongolia Autonomous Region; 2021MS03037 the Natural Science Foundation of Inner Mongolia Autonomous Region; 2022YFE0114400 National Key Research and Development Program of China; 31460658 the National Nature Science Foundation of China; 32160842 the National Nature Science Foundation of China; 2022YFDZ0049 Inner Mongolia autonomous region science and technology plan project; GXKY22042 the Basic scientific research operating expenses of colleges and universities directly under Inner Mongolia Autonomous Region project
فهرسة مساهمة: Keywords: Cysticercus cellulosae; Immunochromatographic strip; Immunodiagnosis; Time-resolved fluorescent microspheres
المشرفين على المادة: 0 (Antibodies, Helminth)
0 (Antigens, Helminth)
تواريخ الأحداث: Date Created: 20240509 Date Completed: 20240509 Latest Revision: 20240513
رمز التحديث: 20240513
مُعرف محوري في PubMed: PMC11077887
DOI: 10.1186/s12917-024-04034-7
PMID: 38720329
قاعدة البيانات: MEDLINE
الوصف
تدمد:1746-6148
DOI:10.1186/s12917-024-04034-7