دورية أكاديمية
Engineering Glucosamine-6-Phosphate Synthase to Achieve Efficient One-Step Biosynthesis of Glucosamine.
| العنوان: | Engineering Glucosamine-6-Phosphate Synthase to Achieve Efficient One-Step Biosynthesis of Glucosamine. |
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| المؤلفون: | Guan ZB; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China., Deng XT; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China., Zhang ZH; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China., Xu GC; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China., Cheng WL; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, People's Republic of China., Liao XR; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China., Cai YJ; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China. |
| المصدر: | ACS chemical biology [ACS Chem Biol] 2024 Jun 21; Vol. 19 (6), pp. 1237-1242. Date of Electronic Publication: 2024 May 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101282906 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-8937 (Electronic) Linking ISSN: 15548929 NLM ISO Abbreviation: ACS Chem Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c2006- |
| مواضيع طبية MeSH: | Glucosamine*/biosynthesis , Glucosamine*/metabolism , Glucosamine*/chemistry , Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)*/metabolism , Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)*/genetics , Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)*/chemistry , Bacillus subtilis*/enzymology , Bacillus subtilis*/metabolism , Bacillus subtilis*/genetics , Protein Engineering*, Molecular Docking Simulation ; Fructose/metabolism ; Fructose/chemistry ; Fructose/biosynthesis ; Molecular Dynamics Simulation ; Bacterial Proteins/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/chemistry ; Catalytic Domain |
| مستخلص: | As an important functional monosaccharide, glucosamine (GlcN) is widely used in fields such as medicine, food nutrition, and health care. Here, we report a distinct GlcN biosynthesis method that utilizes engineered Bacillus subtilis glucosamine-6-phosphate synthase ( Bs GlmS) to convert D-fructose to directly generate GlcN. The best variant obtained by using a combinatorial active-site saturation test/iterative saturation mutagenesis (CAST/ISM) strategy was a quadruple mutant S596D/V597G/S347H/G299Q ( Bs GlmS-BK19), which has a catalytic activity 1736-fold that of the wild type toward D-fructose. Upon using mutant BK19 as a whole-cell catalyst, D-fructose was converted into GlcN with 65.32% conversion in 6 h, whereas the wild type only attained a conversion rate of 0.31% under the same conditions. Molecular docking and molecular dynamics simulations were implemented to provide insights into the mechanism underlying the enhanced activity of BK19. Importantly, the Bs GlmS-BK19 variant specifically catalyzes D-fructose without the need for phosphorylated substrates, representing a significant advancement in GlcN biosynthesis. |
| المشرفين على المادة: | N08U5BOQ1K (Glucosamine) EC 2.6.1.16 (Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)) 30237-26-4 (Fructose) 0 (Bacterial Proteins) |
| تواريخ الأحداث: | Date Created: 20240509 Date Completed: 20240621 Latest Revision: 20240624 |
| رمز التحديث: | 20240624 |
| DOI: | 10.1021/acschembio.4c00144 |
| PMID: | 38723147 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1554-8937 |
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| DOI: | 10.1021/acschembio.4c00144 |