دورية أكاديمية
أعرض في EDS

Protective effects of Wenqingyin on sepsis-induced acute lung injury through regulation of the receptor for advanced glycation end products pathway.

التفاصيل البيبلوغرافية
العنوان: Protective effects of Wenqingyin on sepsis-induced acute lung injury through regulation of the receptor for advanced glycation end products pathway.
المؤلفون: Xie L; Department of Hepatology, Southern Medical University Hospital of Integrated Traditional Chinese and Western Medicine, Southern Medical University, Guangzhou 510315, China., Zhang G; Department of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China., Wu Y; Binzhou Medical University Hospital, Binzhou 256603, China., Hua Y; Department of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China., Ding W; School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China., Han X; Department of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China., Liu B; Department of Traditional Chinese Medicine, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510260, China. Electronic address: xmhoolv@163.com., Zhou C; Department of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: zchuying_tcm@163.com., Li A; Department of Hepatology, Southern Medical University Hospital of Integrated Traditional Chinese and Western Medicine, Southern Medical University, Guangzhou 510315, China. Electronic address: liaimin2005@163.com.
المصدر: Phytomedicine : international journal of phytotherapy and phytopharmacology [Phytomedicine] 2024 Jul; Vol. 129, pp. 155654. Date of Electronic Publication: 2024 Apr 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Urban & Fischer Verlag Country of Publication: Germany NLM ID: 9438794 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-095X (Electronic) Linking ISSN: 09447113 NLM ISO Abbreviation: Phytomedicine Subsets: MEDLINE
أسماء مطبوعة: Publication: Stuttgart : Urban & Fischer Verlag
Original Publication: Stuttgart ; New York : G. Fischer, c1994-
مواضيع طبية MeSH: Acute Lung Injury*/drug therapy , Sepsis*/complications , Sepsis*/drug therapy , Drugs, Chinese Herbal*/pharmacology , Receptor for Advanced Glycation End Products*/metabolism , Signal Transduction*/drug effects , Lipopolysaccharides*, Animals ; Mice ; RAW 264.7 Cells ; Male ; Cytokines/metabolism ; Mice, Inbred C57BL ; Disease Models, Animal ; Network Pharmacology ; Protective Agents/pharmacology ; Glycation End Products, Advanced/metabolism
مستخلص: Background: Wenqingyin (WQY), an ancient Chinese medicinal agent, has been extensively used in treating infectious ailments throughout history. However, the anti-sepsis mechanism remains unknown.
Purpose: This study investigated the diverse mechanisms of WQY in mitigating sepsis-induced acute lung injury (ALI). Additionally, the effects of WQY were validated using biological experiments.
Methods: This study combined UHPLC-Orbitrap-HRMS analysis and network pharmacology to predict the potential anti-sepsis mechanism of WQY. Sepsis-induced ALI models were established in vivo via intraperitoneal lipopolysaccharide (LPS) administration and in vitro by LPS-stimulated RAW 264.7 macrophages. Various techniques, including hematoxylin-eosin staining, TUNEL, qPCR, and ELISA, were used to assess lung damage and quantify inflammatory cytokines. Inflammatory cell infiltration was visualized through immunohistochemistry. Hub targets and signaling pathways were identified using Western blotting, immunohistochemistry, and immunofluorescence staining.
Results: Seventy-five active components and 237 associated targets were acquired, with 145 of these targets overlapping with processes related to sepsis. Based on the comprehensive protein-protein interaction network analysis, JUN, AKT1, TP53, IL-6, HSP90AA1, CASP3, VEGFA, IL-1β, RELA, and EGFR may be targets of WQY for sepsis. Analysis of the Kyoto Gene and Genome Encyclopedia revealed that WQY is implicated in the advanced glycation end products/receptor for advanced glycation end products (AGE/RAGE) signaling pathway. In vivo, WQY alleviated sepsis-induced ALI, suppressing proinflammatory cytokines and inhibiting macrophage/neutrophil infiltration. In vitro, WQY reduced TNF-α, IL-6, and IL-1β in LPS-induced RAW 264.7 macrophages. Furthermore, we verified that WQY protected against sepsis-induced ALI by regulating the RAGE pathway for the first time. Baicalin, coptisine, and paeoniflorin may be the effective components of WQY that inhibit RAGE.
Conclusion: The primary mechanism of WQY in combating sepsis-induced ALI involves controlling RAGE levels and the PI3K/AKT pathway, suppressing inflammation, and mitigating lung damage. This study establishes a scientific foundation for understanding the mechanism of WQY and its clinical use in treating sepsis.
Competing Interests: Declaration of competing interest The authors declared that they have no conflicts of interest to this work. We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.
(Copyright © 2024 Elsevier GmbH. All rights reserved.)
فهرسة مساهمة: Keywords: Acute lung injury; Network pharmacology; RAGE; Sepsis; Wenqingyin
المشرفين على المادة: 0 (Drugs, Chinese Herbal)
0 (Receptor for Advanced Glycation End Products)
0 (Lipopolysaccharides)
0 (Cytokines)
0 (Protective Agents)
0 (Glycation End Products, Advanced)
تواريخ الأحداث: Date Created: 20240509 Date Completed: 20240529 Latest Revision: 20240705
رمز التحديث: 20240705
DOI: 10.1016/j.phymed.2024.155654
PMID: 38723525
قاعدة البيانات: MEDLINE
الوصف
تدمد:1618-095X
DOI:10.1016/j.phymed.2024.155654