دورية أكاديمية
أعرض في EDS

Antibiotic-induced microbiome depletion promotes intestinal colonization by Campylobacter jejuni in mice.

التفاصيل البيبلوغرافية
العنوان: Antibiotic-induced microbiome depletion promotes intestinal colonization by Campylobacter jejuni in mice.
المؤلفون: Chen H; Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, No. 1188 Wuzhou Street, Wucheng District, Jinhua, Zhejiang Province, P.R. China. 20101044@jhc.edu.cn., Zhang Y; Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, No. 1188 Wuzhou Street, Wucheng District, Jinhua, Zhejiang Province, P.R. China., Pan Y; Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, No. 1188 Wuzhou Street, Wucheng District, Jinhua, Zhejiang Province, P.R. China., Wu L; Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, No. 1188 Wuzhou Street, Wucheng District, Jinhua, Zhejiang Province, P.R. China., Wang W; Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, No. 1188 Wuzhou Street, Wucheng District, Jinhua, Zhejiang Province, P.R. China., Zhang H; Animal Center, Jinhua Food and Drug Inspection and Testing Research Institute, Jinhua, Zhejiang Province, P.R. China., Lou H; Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, No. 1188 Wuzhou Street, Wucheng District, Jinhua, Zhejiang Province, P.R. China. 20050059@jhc.edu.cn.
المصدر: BMC microbiology [BMC Microbiol] 2024 May 09; Vol. 24 (1), pp. 156. Date of Electronic Publication: 2024 May 09.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Campylobacter jejuni*/drug effects , Campylobacter jejuni*/growth & development , Anti-Bacterial Agents*/pharmacology , Mice, Inbred C57BL* , Cefoperazone*/pharmacology , Feces*/microbiology , Campylobacter Infections*/microbiology , Gastrointestinal Microbiome*/drug effects , Sulbactam*/pharmacology , RNA, Ribosomal, 16S*/genetics, Animals ; Female ; Mice ; Intestines/microbiology ; Colon/microbiology ; Colon/pathology ; Disease Models, Animal ; Intestinal Mucosa/microbiology ; Intestinal Mucosa/drug effects ; DNA, Bacterial/genetics ; DNA, Ribosomal/genetics
مستخلص: Background: To establish a method to induce Campylobacter jejuni colonization in the intestines of C57BL/6 mice through antibiotic-induced microbiome depletion.
Results: Fifty-four female C57BL/6 mice were divided into the normal, control, and experimental groups. The experimental group was administered intragastric cefoperazone sodium and sulbactam sodium (50 mg/mL) for 2 days; then, the experimental and control mice were intragastrically administered 200 µL C. jejuni, which was repeated once more after 2 days. Animal feces were collected, and the HipO gene of C. jejuni was detected using TaqMan qPCR from day 1 to day 14 after modeling completion. Immunofluorescence was used to detect intestinal C. jejuni colonization on day 14, and pathological changes were observed using hematoxylin and eosin staining. Additionally, 16S rDNA analyses of the intestinal contents were conducted on day 14. In the experimental group, C. jejuni was detected in the feces from days 1 to 14 on TaqMan qPCR, and immunofluorescence-labeled C. jejuni were visibly discernable in the intestinal lumen. The intestinal mucosa was generally intact and showed no significant inflammatory-cell infiltration. Diversity analysis of the colonic microbiota showed significant inter-group differences. In the experimental group, the composition of the colonic microbiota differed from that in the other 2 groups at the phylum level, and was characterized by a higher proportion of Bacteroidetes and a lower proportion of Firmicutes.
Conclusions: Microbiome depletion induced by cefoperazone sodium and sulbactam sodium could promote long-term colonization of C. jejuni in the intestines of mice.
(© 2024. The Author(s).)
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معلومات مُعتمدة: LGD22C040006 and LTGY24H190003 Basic Public Welfare Research Project of Zhejiang Province; 2021-4-112 Science and Technology Project of Jinhua City
فهرسة مساهمة: Keywords: Campylobacter jejuni; 16S rDNA analysis; Colonization; TaqMan qPCR
المشرفين على المادة: 0 (Anti-Bacterial Agents)
7U75I1278D (Cefoperazone)
S4TF6I2330 (Sulbactam)
0 (RNA, Ribosomal, 16S)
0 (DNA, Bacterial)
0 (DNA, Ribosomal)
تواريخ الأحداث: Date Created: 20240509 Date Completed: 20240510 Latest Revision: 20240512
رمز التحديث: 20240512
مُعرف محوري في PubMed: PMC11080253
DOI: 10.1186/s12866-024-03313-5
PMID: 38724913
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2180
DOI:10.1186/s12866-024-03313-5