دورية أكاديمية
Characterization, histopathology and immunogenicity of the lumpy skin disease virus isolated during 2019-20 in Bangladesh.
| العنوان: | Characterization, histopathology and immunogenicity of the lumpy skin disease virus isolated during 2019-20 in Bangladesh. |
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| المؤلفون: | Uddin MA; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Hossain MT; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Rahman AKMA; Department of Medicine, Bangladesh Agricultural University, Mymensingh, Bangladesh., Siddique MP; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Kafi MA; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Hossain MG; Department of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh., Chakraborty S; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Rahman MM; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Khasruzzaman AKM; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Ward MP; Sydney School of Veterinary Science, The University of Sydney, Camden, NSW, Australia., Islam MA; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh. |
| المصدر: | Frontiers in microbiology [Front Microbiol] 2024 Apr 25; Vol. 15, pp. 1324243. Date of Electronic Publication: 2024 Apr 25 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
| مستخلص: | Introduction: Lumpy skin disease (LSD) is a highly contagious vector-borne viral disease of cattle. LSD has emerged in Bangladesh in 2019, causing significant economic losses due to its high morbidity and mortality. This research was designed to isolate, identify, and assess the immunogenicity of LSD virus (LSDV) using nodular tissue samples obtained from affected cattle during the 2019-20 outbreak across nine districts of Bangladesh. Methods: To determine the presence of LSDV in nodular tissues, we initially used iiPCR and PCR, followed by histopathological examination. 151 were positive via iiPCR and PCR among the 180 collected samples. The PCR positive 151 samples were then inoculated into 10-day-old embryonated chicken eggs via the CAM route to isolate LSDV, confirmed through PCR. Subsequently, partial sequencing and phylogenetic analysis of the P32 gene were performed to determine the origin of the circulating LSDV strain. The immunogenicity of selected LSDV strains was assessed through an ELISA test. Results: The PCR results revealed a distinct positive band at 192 bp in both the nodular tissue samples and the LSDV isolated from chicken embryo inoculations. Microscopic analysis of the nodular lesions revealed thickening of the epidermis, ballooning degeneration of keratinocytes, and proliferation of follicular epithelia. Additionally, mononuclear infiltration was observed at the demarcation line between infected and healthy tissue, with necrosis of muscular tissues beneath the epidermis. The LSDV isolate from Bangladesh exhibited a close genetic relationship with LSDV strains isolated from neighboring and other regional countries including India, Myanmar, and Mongolia. This observation strongly suggests the possibility of a transboundary spread of the LSD outbreak in Bangladesh during 2019-2020. The results of the immunogenicity test showed that the serum antibody titer remained at a protective level for up to 18 months following secondary immunization with inactivated LSDV antigen. This finding suggests that the inactivated LSDV antigen could be a potential vaccine candidate to protect cattle in Bangladesh against LSDV. Conclusion: In conclusion, our research successfully isolated, identified, and characterized LSDV in cattle nodular tissues from the 2019-20 outbreak in Bangladesh. Furthermore, it provided insights into the probable origin of the circulating strain and investigated a potential vaccine candidate to protect cattle in the region from LSDV. Competing Interests: The authors extend their heartfelt thanks to the Managing Director of FnF Pharmaceuticals Ltd., Bangladesh, for providing financial support that enabled the execution of this research. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article, or the decision to submit it for publication. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Uddin, Hossain, Rahman, Siddique, Kafi, Hossain, Chakraborty, Rahman, Khasruzzaman, Ward and Islam.) |
| References: | Vet Med Sci. 2021 Sep;7(5):1616-1624. (PMID: 33993641) BMC Vet Res. 2021 Jan 29;17(1):61. (PMID: 33514360) Viruses. 2023 Aug 31;15(9):. (PMID: 37766268) Transbound Emerg Dis. 2017 Dec;64(6):1782-1789. (PMID: 27633121) Transbound Emerg Dis. 2016 Dec;63(6):e288-e295. (PMID: 25754131) Transbound Emerg Dis. 2020 Nov;67(6):2408-2422. (PMID: 32304275) Epidemiol Infect. 1995 Feb;114(1):219-26. (PMID: 7867740) Vet Pathol. 2020 May;57(3):388-396. (PMID: 32314676) Methods Mol Biol. 2011;691:69-82. (PMID: 20972747) Vaccine. 2005 Apr 27;23(23):3061-7. (PMID: 15811653) Vaccines (Basel). 2021 Oct 06;9(10):. (PMID: 34696244) Transbound Emerg Dis. 2016 Oct;63(5):e395-402. (PMID: 25644051) Viruses. 2022 Nov 15;14(11):. (PMID: 36423138) Comp Clin Path. 2021;30(4):693-700. (PMID: 34335135) Onderstepoort J Vet Res. 1969 Dec;36(2):165-74. (PMID: 5408795) Transbound Emerg Dis. 2022 Nov;69(6):3405-3418. (PMID: 36056232) Antiviral Res. 2015 Jul;119:28-35. (PMID: 25907637) Transbound Emerg Dis. 2018 Dec;65(6):1514-1521. (PMID: 29749049) BMC Vet Res. 2019 May 24;15(1):168. (PMID: 31126297) PLoS One. 2012;7(9):e45278. (PMID: 23049781) Transbound Emerg Dis. 2022 Nov;69(6):3397-3404. (PMID: 36053488) Vet Microbiol. 2017 Mar;201:78-84. (PMID: 28284627) J Virol. 2021 Apr 12;95(9):. (PMID: 33568514) Vet World. 2019 Nov;12(11):1826-1832. (PMID: 32009762) Biotechnol J. 2012 May;7(5):662-6. (PMID: 22241586) Vet World. 2015 Sep;8(9):1131-6. (PMID: 27047209) Front Cell Infect Microbiol. 2022 Jul 07;12:948771. (PMID: 35873169) Mol Biol Evol. 2016 Jul;33(7):1870-4. (PMID: 27004904) J Vet Diagn Invest. 1990 Apr;2(2):111-5. (PMID: 1965577) Dev Biol (Basel). 2003;114:161-7. (PMID: 14677686) BMC Vet Res. 2019 Mar 6;15(1):80. (PMID: 30841894) |
| فهرسة مساهمة: | Keywords: LSDV; P32 gene; PCR; embryo inoculation; iELISA; iiPCR; phylogenetic analysis |
| تواريخ الأحداث: | Date Created: 20240510 Latest Revision: 20240511 |
| رمز التحديث: | 20240511 |
| مُعرف محوري في PubMed: | PMC11081030 |
| DOI: | 10.3389/fmicb.2024.1324243 |
| PMID: | 38725689 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-302X |
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| DOI: | 10.3389/fmicb.2024.1324243 |