دورية أكاديمية

Genomic analysis of the first multidrug-resistant ESBL-producing high-risk clonal lineage Klebsiella pneumoniae ST147 isolated from a cat with urinary tract infection.

التفاصيل البيبلوغرافية
العنوان: Genomic analysis of the first multidrug-resistant ESBL-producing high-risk clonal lineage Klebsiella pneumoniae ST147 isolated from a cat with urinary tract infection.
المؤلفون: Sakauchi VTS; Laboratório de Zoonoses Bacterianas, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Haisi A; Instituto de Biotecnologia, Universidade Estadual Paulista (UNESP), Botucatu, Brazil., Araújo Júnior JP; Instituto de Biotecnologia, Universidade Estadual Paulista (UNESP), Botucatu, Brazil., Ferreira Neto JS; Laboratório de Zoonoses Bacterianas, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Heinemann MB; Laboratório de Zoonoses Bacterianas, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Gaeta NC; Laboratório de Zoonoses Bacterianas, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil. natalia.gaeta@usp.br.; Curso de Medicina Veterinária, Faculdades Integradas Campos Salles, São Paulo, Brazil. natalia.gaeta@usp.br.
المصدر: Veterinary research communications [Vet Res Commun] 2024 Aug; Vol. 48 (4), pp. 2783-2788. Date of Electronic Publication: 2024 May 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: Switzerland NLM ID: 8100520 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-7446 (Electronic) Linking ISSN: 01657380 NLM ISO Abbreviation: Vet Res Commun Subsets: MEDLINE
أسماء مطبوعة: Publication: [Cham] : Springer
Original Publication: [Amsterdam] : Elsevier, 1980-
مواضيع طبية MeSH: Klebsiella pneumoniae*/genetics , Klebsiella pneumoniae*/drug effects , Klebsiella pneumoniae*/isolation & purification , Klebsiella pneumoniae*/enzymology , Urinary Tract Infections*/veterinary , Urinary Tract Infections*/microbiology , Cat Diseases*/microbiology , Drug Resistance, Multiple, Bacterial*/genetics , Klebsiella Infections*/veterinary , Klebsiella Infections*/microbiology, Animals ; Cats ; beta-Lactamases/genetics ; beta-Lactamases/metabolism ; Anti-Bacterial Agents/pharmacology ; Phylogeny ; Genome, Bacterial ; Whole Genome Sequencing/veterinary
مستخلص: Urinary tract infections (UTIs) are pervasive in human and veterinary medicine, notably affecting companion animals. These infections frequently lead to the prescription of antibiotics, contributing to the rise of antimicrobial-resistant bacteria. This escalating concern is underscored by the emergence of a previously undocumented case: a high-risk clone, broad-spectrum cephalosporin-resistant K. pneumoniae ST147 strain, denoted USP-275675, isolated from a cat with UTI. Characterized by a multidrug-resistant (MDR) profile, whole genome sequencing exposed several antimicrobial-resistance genes, notably bla CTX-M-15 , bla TEM-1B , bla SHV-11 , and bla OXA-1 . ST147, recognized as a high-risk clone, has historically disseminated globally and is frequently associated with carbapenemases and extended-spectrum β-lactamases. Notably, the core-genome phylogeny of K. pneumoniae ST147 strains isolated from urine samples revealed a unique aspect of the USP-276575 strain. Unlike its counterparts, it did not cluster with other isolates. However, a broader examination incorporating strains from both human and animal sources unveiled a connection between USP-276575 and a Portuguese strain from chicken meat. Both were part of a larger cluster of ST147 strains spanning various geographic locations and sample types, sharing commonalities such as IncFIB or IncR plasmids. This elucidates the MDR signature inherent in widespread K. pneumoniae ST147 strains carrying these plasmids, highlighting their pivotal role in disseminating antimicrobial resistance (AMR). Finally, discovering the high-risk clone K. pneumoniae ST147 in a domestic feline with a UTI in Brazil highlights the urgent need for thorough AMR surveillance through a One Health approach.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
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فهرسة مساهمة: Keywords: Carbapenemases; Cat; Genomic analysis; One health; Surveillance; Urinary tract infection
المشرفين على المادة: EC 3.5.2.6 (beta-Lactamases)
0 (Anti-Bacterial Agents)
تواريخ الأحداث: Date Created: 20240510 Date Completed: 20240809 Latest Revision: 20240815
رمز التحديث: 20240815
DOI: 10.1007/s11259-024-10396-y
PMID: 38727990
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-7446
DOI:10.1007/s11259-024-10396-y