دورية أكاديمية
Fecal shedding of Clostridioides difficile in calves in Sao Paulo state, Brazil.
| العنوان: | Fecal shedding of Clostridioides difficile in calves in Sao Paulo state, Brazil. |
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| المؤلفون: | Cerri FM; Sao Paulo State University (UNESP), School of Veterinary Medicine and Animal Science (FMVZ), Botucatu, Sao Paulo, Brazil., Basso RM; Sao Paulo State University (UNESP), School of Veterinary Medicine and Animal Science (FMVZ), Botucatu, Sao Paulo, Brazil., Pereira WAB; Sao Paulo State University (UNESP), School of Veterinary Medicine and Animal Science (FMVZ), Botucatu, Sao Paulo, Brazil., Silveira JMDS; Federal University of Rio de Janeiro (UFRJ), Paulo de Góes Institute of Microbiology (IMPG), Department of Medical Microbiology, UFRJ, Rio de Janeiro, Rio Janeiro, Brazil., Ferreira EO; Federal University of Rio de Janeiro (UFRJ), Paulo de Góes Institute of Microbiology (IMPG), Department of Medical Microbiology, UFRJ, Rio de Janeiro, Rio Janeiro, Brazil., Haisi A; Sao Paulo State University (UNESP), Institute of Biotechnology (IBTEC), Botucatu, Sao Paulo, Brazil., Araújo Júnior JP; Sao Paulo State University (UNESP), Institute of Biotechnology (IBTEC), Botucatu, Sao Paulo, Brazil., Arroyo LG; University of Guelph, Ontario Veterinary College, Department of Clinical Studies, Guelph, Ontario, Canada., de Castro YG; Federal University of Minas Gerais (UFMG), School of Veterinary Medicine (EV), Belo Horizonte, Minas Gerais, Brazil., Silva ROS; Federal University of Minas Gerais (UFMG), School of Veterinary Medicine (EV), Belo Horizonte, Minas Gerais, Brazil., Oliveira-Filho JP; Sao Paulo State University (UNESP), School of Veterinary Medicine and Animal Science (FMVZ), Botucatu, Sao Paulo, Brazil., Borges AS; Sao Paulo State University (UNESP), School of Veterinary Medicine and Animal Science (FMVZ), Botucatu, Sao Paulo, Brazil. Electronic address: alexandre.s.borges@unesp.br. |
| المصدر: | Anaerobe [Anaerobe] 2024 May 09; Vol. 88, pp. 102861. Date of Electronic Publication: 2024 May 09. |
| Publication Model: | Ahead of Print |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 9505216 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-8274 (Electronic) Linking ISSN: 10759964 NLM ISO Abbreviation: Anaerobe Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Academic Press Original Publication: London ; San Diego : Academic Press, c1995- |
| مستخلص: | Objective: This study aimed to evaluate the fecal shedding of C. difficile in calves on farms in Sao Paulo State, Brazil. Materials and Methods: Fecal samples (n = 300) were collected from diarrheic (n = 78) and nondiarrheic (n = 222) calves less than 60 days of age from 20 farms. Fecal samples were inoculated into enrichment broth supplemented with taurocholate and cultured under anaerobic conditions. Colonies suspected to be C. difficile were harvested for DNA extraction and then multiplex PCR for the detection of genes encoding toxins A and B and binary toxins. All toxigenic isolates were ribotyped and tested for antimicrobial susceptibility, and five selected strains were subjected to whole-genome sequencing to determine their sequence type. Results and Discussion: C. difficile was isolated from 29.3 % (88/300) of the samples. All toxigenic isolates (17/88, 19.3 %) were classified as ribotypes RT046 (13/17-79.47 %, A + B + CDT - ) and RT126 (4/17 = 20.53 %, A + B + CDT + ). The sequenced strains from RT046 were classified as ST35 (Clade 1), while those from RT126 were classified as ST11 (Clade 5). No associations between the epidemiological factors in any of the groups and C. difficile isolation were observed. Most of the toxigenic isolates (16/17 = 94.41 %) were classified as multidrug-resistant. Calves can be an important source of toxigenic C. difficile strains, including multidrug-resistant isolates from ribotypes commonly observed in humans. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Animals; Cattle; Diarrhea; MLST; RT046; RT126; ST35 |
| تواريخ الأحداث: | Date Created: 20240510 Latest Revision: 20240710 |
| رمز التحديث: | 20240711 |
| DOI: | 10.1016/j.anaerobe.2024.102861 |
| PMID: | 38729514 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-8274 |
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| DOI: | 10.1016/j.anaerobe.2024.102861 |