دورية أكاديمية
Inhibitory Subpopulations in preBötzinger Complex Play Distinct Roles in Modulating Inspiratory Rhythm and Pattern.
| العنوان: | Inhibitory Subpopulations in preBötzinger Complex Play Distinct Roles in Modulating Inspiratory Rhythm and Pattern. |
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| المؤلفون: | Chang Z; Stanson Toshok Center for Brain Function and Repair, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois 60064., Skach J; Stanson Toshok Center for Brain Function and Repair, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois 60064., Kam K; Stanson Toshok Center for Brain Function and Repair, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois 60064 kaiwen.kam@rosalindfranklin.edu. |
| المصدر: | The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2024 Jun 19; Vol. 44 (25). Date of Electronic Publication: 2024 Jun 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Society for Neuroscience Country of Publication: United States NLM ID: 8102140 Publication Model: Electronic Cited Medium: Internet ISSN: 1529-2401 (Electronic) Linking ISSN: 02706474 NLM ISO Abbreviation: J Neurosci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington, DC : Society for Neuroscience Original Publication: [Baltimore, Md.] : The Society, c1981- |
| مواضيع طبية MeSH: | Inhalation*/physiology, Animals ; Mice ; Female ; Male ; Neural Inhibition/physiology ; Medulla Oblongata/physiology ; Medulla Oblongata/cytology ; Glutamate Decarboxylase/genetics ; Glutamate Decarboxylase/metabolism ; Mice, Transgenic ; Glycine Plasma Membrane Transport Proteins/genetics ; Glycine Plasma Membrane Transport Proteins/metabolism ; Respiratory Center/physiology ; Respiratory Center/cytology ; Neurons/physiology ; Periodicity ; Animals, Newborn |
| مستخلص: | Inhibitory neurons embedded within mammalian neural circuits shape breathing, walking, and other rhythmic motor behaviors. At the core of the neural circuit controlling breathing is the preBötzinger Complex (preBötC), where GABAergic (GAD1/2 + ) and glycinergic (GlyT2 + ) neurons are functionally and anatomically intercalated among glutamatergic Dbx1-derived (Dbx1 + ) neurons that generate rhythmic inspiratory drive. The roles of these preBötC inhibitory neurons in breathing remain unclear. We first characterized the spatial distribution of molecularly defined preBötC inhibitory subpopulations in male and female neonatal double reporter mice expressing either tdTomato or EGFP in GlyT2 + , GAD1 + , or GAD2 + neurons. We found that the majority of preBötC inhibitory neurons expressed both GlyT2 and GAD2 while a much smaller subpopulation also expressed GAD1. To determine the functional role of these subpopulations, we used holographic photostimulation, a patterned illumination technique, in rhythmically active medullary slices from neonatal Dbx1 tdTomato ;GlyT2 EGFP and Dbx1 tdTomato ;GAD1 EGFP double reporter mice of either sex. Stimulation of 4 or 8 preBötC GlyT2 + neurons during endogenous rhythm prolonged the interburst interval in a phase-dependent manner and increased the latency to burst initiation when bursts were evoked by stimulation of Dbx1 + neurons. In contrast, stimulation of 4 or 8 preBötC GAD1 + neurons did not affect interburst interval or latency to burst initiation. Instead, photoactivation of GAD1 + neurons during the inspiratory burst prolonged endogenous and evoked burst duration and decreased evoked burst amplitude. We conclude that GlyT2 + /GAD2 + neurons modulate breathing rhythm by delaying burst initiation while a smaller GAD1 + subpopulation shapes inspiratory patterning by altering burst duration and amplitude. Competing Interests: The authors declare no competing financial interests. (Copyright © 2024 the authors.) |
| التعليقات: | Update of: bioRxiv. 2023 Sep 01:2023.08.07.552303. doi: 10.1101/2023.08.07.552303. (PMID: 37609332) |
| References: | J Physiol. 2002 Sep 1;543(Pt 2):643-53. (PMID: 12205196) Eur J Neurosci. 2006 Feb;23(3):667-74. (PMID: 16487148) J Neurophysiol. 2007 Dec;98(6):3370-87. (PMID: 17913982) eNeuro. 2015 Aug 31;2(4):. (PMID: 26465010) Annu Rev Neurosci. 2022 Jul 8;45:223-247. (PMID: 35259917) Nature. 2010 Nov 11;468(7321):263-9. (PMID: 21068835) J Neurosci. 2016 Mar 23;36(12):3471-80. (PMID: 27013676) Genome Biol. 2006;7(10):R100. (PMID: 17076895) J Appl Physiol (1985). 2006 Aug;101(2):618-27. (PMID: 16645192) Behav Res Methods. 2009 Nov;41(4):1149-60. (PMID: 19897823) Elife. 2019 Dec 16;8:. (PMID: 31841107) Proc Natl Acad Sci U S A. 2010 Oct 19;107(42):18208-13. (PMID: 20921395) Neuron. 2020 May 6;106(3):482-497.e4. (PMID: 32130872) J Neurosci. 2010 Nov 3;30(44):14883-95. (PMID: 21048147) Trends Neurosci. 2014 Jul;37(7):370-80. (PMID: 24890196) Neuron. 2015 Aug 19;87(4):684-98. (PMID: 26291155) Nat Neurosci. 2010 Jan;13(1):133-40. (PMID: 20023653) J Neurosci. 2023 Jan 11;43(2):240-260. (PMID: 36400528) Pflugers Arch. 2009 Jul;458(3):459-69. (PMID: 19238427) J Neurosci. 2010 Mar 10;30(10):3634-9. (PMID: 20219997) Respir Physiol Neurobiol. 2023 May;311:104032. (PMID: 36758781) J Neurophysiol. 2018 Jan 1;119(1):96-117. (PMID: 28978767) Annu Rev Neurosci. 2018 Jul 8;41:475-499. (PMID: 29709210) Neuron. 2016 Apr 6;90(1):86-100. (PMID: 27021171) J Physiol. 2015 Jan 1;593(1):3-23. (PMID: 25556783) Front Physiol. 2018 Sep 03;9:1219. (PMID: 30233397) J Neurosci. 2013 Feb 20;33(8):3332-8. (PMID: 23426661) Int J Mol Sci. 2021 Feb 18;22(4):. (PMID: 33670653) J Neurosci. 2013 Mar 27;33(13):5454-65. (PMID: 23536061) Neuron. 2011 Oct 20;72(2):231-43. (PMID: 22017986) Proc Natl Acad Sci U S A. 2015 Aug 4;112(31):9728-33. (PMID: 26195782) J Neurosci. 2013 Feb 13;33(7):2994-3009. (PMID: 23407957) Nat Neurosci. 2005 Aug;8(8):1002-12. (PMID: 16041369) Respir Physiol Neurobiol. 2018 Dec;258:104-107. (PMID: 25644207) Respir Physiol Neurobiol. 2019 Jul;265:141-152. (PMID: 30395936) Neuron. 1991 Jul;7(1):91-100. (PMID: 2069816) Neuron. 2011 Sep 22;71(6):995-1013. (PMID: 21943598) Proc Natl Acad Sci U S A. 2005 Aug 30;102(35):12560-5. (PMID: 16116096) Nat Neurosci. 2015 Mar;18(3):408-14. (PMID: 25643296) PLoS One. 2010 Feb 25;5(2):e9431. (PMID: 20195547) J Neurosci. 2013 May 29;33(22):9235-45. (PMID: 23719793) Nat Commun. 2018 Feb 26;9(1):843. (PMID: 29483589) J Neurophysiol. 2022 Jul 1;128(1):181-196. (PMID: 35675444) J Neural Eng. 2009 Dec;6(6):066004. (PMID: 19837999) J Neurosci. 2003 Oct 1;23(26):8941-8. (PMID: 14523096) J Neurosci. 2004 Oct 27;24(43):9598-611. (PMID: 15509747) Neuron. 2015 Mar 18;85(6):1289-304. (PMID: 25789756) J Neurophysiol. 1997 Apr;77(4):1853-60. (PMID: 9114241) Front Cell Neurosci. 2019 Nov 19;13:517. (PMID: 31803026) Science. 1991 Nov 1;254(5032):726-9. (PMID: 1683005) Elife. 2022 Apr 05;11:. (PMID: 35380537) J Neurosci. 2008 Mar 5;28(10):2353-65. (PMID: 18322082) J Comp Neurol. 2005 Feb 7;482(2):123-41. (PMID: 15611994) Nat Methods. 2008 Sep;5(9):821-7. (PMID: 19160517) Compr Physiol. 2012 Oct;2(4):2443-69. (PMID: 23720253) Sci Rep. 2017 Aug 17;7(1):8669. (PMID: 28819234) Nat Neurosci. 2010 Sep;13(9):1066-74. (PMID: 20680010) Philos Trans R Soc Lond B Biol Sci. 2015 Sep 19;370(1677):20140204. (PMID: 26240419) Neuroscience. 2004;127(2):409-17. (PMID: 15262331) eNeuro. 2016 May 13;3(2):. (PMID: 27200412) Sci Rep. 2023 Jan 11;13(1):586. (PMID: 36631589) J Comp Neurol. 2018 Jun 1;526(8):1389-1402. (PMID: 29473167) J Histochem Cytochem. 1985 Aug;33(8):755-61. (PMID: 3926864) J Physiol. 2015 Jul 15;593(14):3033-46. (PMID: 25384785) Physiol Rep. 2014 Aug 19;2(8):. (PMID: 25138790) Front Neural Circuits. 2010 Nov 29;4:124. (PMID: 21151816) Front Neuroanat. 2020 Sep 04;14:58. (PMID: 33013329) |
| معلومات مُعتمدة: | R01 NS097492 United States NS NINDS NIH HHS |
| فهرسة مساهمة: | Keywords: breathing; central pattern generator; inhibition; motor systems |
| المشرفين على المادة: | EC 4.1.1.15 (Glutamate Decarboxylase) 0 (Glycine Plasma Membrane Transport Proteins) EC 4.1.1.15 (glutamate decarboxylase 1) 0 (Slc6a5 protein, mouse) EC 4.1.1.15 (glutamate decarboxylase 2) |
| تواريخ الأحداث: | Date Created: 20240510 Date Completed: 20240619 Latest Revision: 20240629 |
| رمز التحديث: | 20240629 |
| مُعرف محوري في PubMed: | PMC11209648 |
| DOI: | 10.1523/JNEUROSCI.1928-23.2024 |
| PMID: | 38729762 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1529-2401 |
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| DOI: | 10.1523/JNEUROSCI.1928-23.2024 |