دورية أكاديمية
Inhibitory Subpopulations in preBötzinger Complex Play Distinct Roles in Modulating Inspiratory Rhythm and Pattern.
العنوان: | Inhibitory Subpopulations in preBötzinger Complex Play Distinct Roles in Modulating Inspiratory Rhythm and Pattern. |
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المؤلفون: | Chang Z; Stanson Toshok Center for Brain Function and Repair, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois 60064., Skach J; Stanson Toshok Center for Brain Function and Repair, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois 60064., Kam K; Stanson Toshok Center for Brain Function and Repair, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois 60064 kaiwen.kam@rosalindfranklin.edu. |
المصدر: | The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2024 Jun 19; Vol. 44 (25). Date of Electronic Publication: 2024 Jun 19. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Society for Neuroscience Country of Publication: United States NLM ID: 8102140 Publication Model: Electronic Cited Medium: Internet ISSN: 1529-2401 (Electronic) Linking ISSN: 02706474 NLM ISO Abbreviation: J Neurosci Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Washington, DC : Society for Neuroscience Original Publication: [Baltimore, Md.] : The Society, c1981- |
مواضيع طبية MeSH: | Inhalation*/physiology, Animals ; Mice ; Female ; Male ; Neural Inhibition/physiology ; Medulla Oblongata/physiology ; Medulla Oblongata/cytology ; Glutamate Decarboxylase/genetics ; Glutamate Decarboxylase/metabolism ; Mice, Transgenic ; Glycine Plasma Membrane Transport Proteins/genetics ; Glycine Plasma Membrane Transport Proteins/metabolism ; Respiratory Center/physiology ; Respiratory Center/cytology ; Neurons/physiology ; Periodicity ; Animals, Newborn |
مستخلص: | Inhibitory neurons embedded within mammalian neural circuits shape breathing, walking, and other rhythmic motor behaviors. At the core of the neural circuit controlling breathing is the preBötzinger Complex (preBötC), where GABAergic (GAD1/2 + ) and glycinergic (GlyT2 + ) neurons are functionally and anatomically intercalated among glutamatergic Dbx1-derived (Dbx1 + ) neurons that generate rhythmic inspiratory drive. The roles of these preBötC inhibitory neurons in breathing remain unclear. We first characterized the spatial distribution of molecularly defined preBötC inhibitory subpopulations in male and female neonatal double reporter mice expressing either tdTomato or EGFP in GlyT2 + , GAD1 + , or GAD2 + neurons. We found that the majority of preBötC inhibitory neurons expressed both GlyT2 and GAD2 while a much smaller subpopulation also expressed GAD1. To determine the functional role of these subpopulations, we used holographic photostimulation, a patterned illumination technique, in rhythmically active medullary slices from neonatal Dbx1 tdTomato ;GlyT2 EGFP and Dbx1 tdTomato ;GAD1 EGFP double reporter mice of either sex. Stimulation of 4 or 8 preBötC GlyT2 + neurons during endogenous rhythm prolonged the interburst interval in a phase-dependent manner and increased the latency to burst initiation when bursts were evoked by stimulation of Dbx1 + neurons. In contrast, stimulation of 4 or 8 preBötC GAD1 + neurons did not affect interburst interval or latency to burst initiation. Instead, photoactivation of GAD1 + neurons during the inspiratory burst prolonged endogenous and evoked burst duration and decreased evoked burst amplitude. We conclude that GlyT2 + /GAD2 + neurons modulate breathing rhythm by delaying burst initiation while a smaller GAD1 + subpopulation shapes inspiratory patterning by altering burst duration and amplitude. Competing Interests: The authors declare no competing financial interests. (Copyright © 2024 the authors.) |
التعليقات: | Update of: bioRxiv. 2023 Sep 01:2023.08.07.552303. doi: 10.1101/2023.08.07.552303. (PMID: 37609332) |
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معلومات مُعتمدة: | R01 NS097492 United States NS NINDS NIH HHS |
فهرسة مساهمة: | Keywords: breathing; central pattern generator; inhibition; motor systems |
المشرفين على المادة: | EC 4.1.1.15 (Glutamate Decarboxylase) 0 (Glycine Plasma Membrane Transport Proteins) EC 4.1.1.15 (glutamate decarboxylase 1) 0 (Slc6a5 protein, mouse) EC 4.1.1.15 (glutamate decarboxylase 2) |
تواريخ الأحداث: | Date Created: 20240510 Date Completed: 20240619 Latest Revision: 20240629 |
رمز التحديث: | 20240629 |
مُعرف محوري في PubMed: | PMC11209648 |
DOI: | 10.1523/JNEUROSCI.1928-23.2024 |
PMID: | 38729762 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1529-2401 |
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DOI: | 10.1523/JNEUROSCI.1928-23.2024 |