دورية أكاديمية
أعرض في EDS

Effect of Helichrysum italicum in Promoting Collagen Deposition and Skin Regeneration in a New Dynamic Model of Skin Wound Healing.

التفاصيل البيبلوغرافية
العنوان: Effect of Helichrysum italicum in Promoting Collagen Deposition and Skin Regeneration in a New Dynamic Model of Skin Wound Healing.
المؤلفون: Serra D; Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy.; R&D Laboratory Center, InoCure s.r.o, Politickych veziu 935/13, Nové Mesto, Praha 1, 110 00 Prague, Czech Republic., Cruciani S; Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy., Garroni G; Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy., Sarais G; Department of Life and Environmental Sciences, University of Cagliari, University Campus, 09042 Cagliari, Italy., Kavak FF; Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy., Satta R; Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy., Montesu MA; Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy., Floris M; Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy., Ventura C; Laboratory of Molecular Biology and Stem Cell Engineering-Eldor Lab, Istituto Nazionale di Biostrutture e Biosistemi (INBB), 40128 Bologna, Italy., Maioli M; Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy.; Center for Developmental Biology and Reprogramming-CEDEBIOR, Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2024 Apr 26; Vol. 25 (9). Date of Electronic Publication: 2024 Apr 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Wound Healing*/drug effects , Collagen*/metabolism , Skin*/metabolism , Skin*/drug effects , Helichrysum*/chemistry , Plant Extracts*/pharmacology , Regeneration*/drug effects, Humans ; Fibroblasts/metabolism ; Fibroblasts/drug effects ; Stem Cells/metabolism ; Stem Cells/drug effects ; Stem Cells/cytology ; Cells, Cultured
مستخلص: Natural products have many healing effects on the skin with minimal or no adverse effects. In this study, we analyzed the regenerative properties of a waste product (hydrolate) derived from Helichrysum italicum ( HH ) on scratch-tested skin cell populations seeded on a fluidic culture system. Helichrysum italicum has always been recognized in the traditional medicine of Mediterranean countries for its wide pharmacological activities. We recreated skin physiology with a bioreactor that mimics skin stem cell (SSCs) and fibroblast (HFF1) communication as in vivo skin layers. Dynamic culture models represent an essential instrument for recreating and preserving the complex multicellular organization and interactions of the cellular microenvironment. Both cell types were exposed to two different concentrations of HH after the scratch assay and were compared to untreated control cells. Collagen is the constituent of many wound care products that act directly on the damaged wound environment. We analyzed the role played by HH in stimulating collagen production during tissue repair, both in static and dynamic culture conditions, by a confocal microscopic analysis. In addition, we performed a gene expression analysis that revealed the activation of a molecular program of stemness in treated skin stem cells. Altogether, our results indicate a future translational application of this natural extract to support skin regeneration and define a new protocol to recreate a dynamic process of healing.
Competing Interests: The authors declare no conflicts of interest.
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فهرسة مساهمة: Keywords: Helichrysum italicum; bioreactor; dynamic cultures; fibroblasts; molecular mechanisms; stem cells; tissue regeneration; wound healing
المشرفين على المادة: 9007-34-5 (Collagen)
0 (Plant Extracts)
تواريخ الأحداث: Date Created: 20240511 Date Completed: 20240511 Latest Revision: 20240610
رمز التحديث: 20240610
مُعرف محوري في PubMed: PMC11083432
DOI: 10.3390/ijms25094736
PMID: 38731954
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25094736