دورية أكاديمية
أعرض في EDS

Expression and Localization Profiles of Tight Junction Proteins in Immune Cells Depend on Their Activation Status.

التفاصيل البيبلوغرافية
العنوان: Expression and Localization Profiles of Tight Junction Proteins in Immune Cells Depend on Their Activation Status.
المؤلفون: Voges L; Clinical Physiology/Nutritional Medicine, Charité-Universitätsmedizin Berlin, 12203 Berlin, Germany., Weiß F; Clinical Physiology/Nutritional Medicine, Charité-Universitätsmedizin Berlin, 12203 Berlin, Germany., Branco AT; Institute of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, 12203 Berlin, Germany., Fromm M; Clinical Physiology/Nutritional Medicine, Charité-Universitätsmedizin Berlin, 12203 Berlin, Germany., Krug SM; Clinical Physiology/Nutritional Medicine, Charité-Universitätsmedizin Berlin, 12203 Berlin, Germany.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2024 Apr 29; Vol. 25 (9). Date of Electronic Publication: 2024 Apr 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Tight Junction Proteins*/metabolism , Transcription Factors* , Receptors, Lipoprotein* , Granulocytes*/metabolism , Macrophages*/metabolism , Immune System*/metabolism, Humans ; Colon ; Organoids ; HT29 Cells ; Primary Cell Culture
مستخلص: The ability of the immune system to combat pathogens relies on processes like antigen sampling by dendritic cells and macrophages migrating through endo- and epithelia or penetrating them with their dendrites. In addition, other immune cell subtypes also migrate through the epithelium after activation. For paracellular migration, interactions with tight junctions (TJs) are necessary, and previous studies reported TJ protein expression in several immune cells. Our investigation aimed to characterize, in more detail, the expression profiles of TJ proteins in different immune cells in both naïve and activated states. The mRNA expression analysis revealed distinct expression patterns for TJ proteins, with notable changes, mainly increases, upon activation. At the protein level, LSR appeared predominant, being constitutively present in naïve cell membranes, suggesting roles as a crucial interaction partner. Binding experiments suggested the presence of claudins in the membrane only after stimulation, and claudin-8 translocation to the membrane occurred after stimulation. Our findings suggest a dynamic TJ protein expression in immune cells, implicating diverse functions in response to stimulation, like interaction with TJ proteins or regulatory roles. While further analysis is needed to elucidate the precise roles of TJ proteins, our findings indicate important non-canonical functions of TJ proteins in immune response.
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معلومات مُعتمدة: GRK 2318-C2, grant number 318905415 Deutsche Forschungsgemeinschaft; DFG TRR-241-B06, grant number 375876048 Deutsche Forschungsgemeinschaft
فهرسة مساهمة: Keywords: LSR; TAMP; angulin; claudins; immune cells; tight junction proteins
المشرفين على المادة: 0 (Tight Junction Proteins)
0 (LSR protein, human)
0 (Transcription Factors)
0 (Receptors, Lipoprotein)
تواريخ الأحداث: Date Created: 20240511 Date Completed: 20240718 Latest Revision: 20240718
رمز التحديث: 20240719
مُعرف محوري في PubMed: PMC11084252
DOI: 10.3390/ijms25094861
PMID: 38732086
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25094861