دورية أكاديمية
Chickpea (Cicer arietinum) PHO1 family members function redundantly in Pi transport and root nodulation.
| العنوان: | Chickpea (Cicer arietinum) PHO1 family members function redundantly in Pi transport and root nodulation. |
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| المؤلفون: | Mani B; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India., Maurya K; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India., Kohli PS; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India., Giri J; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India. Electronic address: jitender@nipgr.ac.in. |
| المصدر: | Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Jun; Vol. 211, pp. 108712. Date of Electronic Publication: 2024 May 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: France NLM ID: 9882449 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2690 (Electronic) Linking ISSN: 09819428 NLM ISO Abbreviation: Plant Physiol Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Science Original Publication: Paris : Gauthier-Villars, c1987- |
| مواضيع طبية MeSH: | Cicer*/genetics , Cicer*/metabolism , Cicer*/growth & development , Plant Proteins*/genetics , Plant Proteins*/metabolism , Plant Root Nodulation*/genetics, Gene Expression Regulation, Plant ; Phosphates/metabolism ; Phosphate Transport Proteins/metabolism ; Phosphate Transport Proteins/genetics ; Plant Roots/metabolism ; Plant Roots/genetics ; Plant Roots/growth & development ; Phylogeny ; Biological Transport/genetics ; Multigene Family |
| مستخلص: | Phosphorus (P), a macronutrient, plays key roles in plant growth, development, and yield. Phosphate (Pi) transporters (PHTs) and PHOSPHATE1 (PHO1) are central to Pi acquisition and distribution. Potentially, PHO1 is also involved in signal transduction under low P. The current study was designed to identify and functionally characterize the PHO1 gene family in chickpea (CaPHO1s). Five CaPHO1 genes were identified through a comprehensive genome-wide search. Phylogenetically, CaPHO1s formed two clades, and protein sequence analyses confirmed the presence of conserved domains. CaPHO1s are expressed in different plant organs including root nodules and are induced by Pi-limiting conditions. Functional complementation of atpho1 mutant with three CaPHO1 members, CaPHO1, CaPHO1;like, and CaPHO1;H1, independently demonstrated their role in root to shoot Pi transport, and their redundant functions. To further validate this, we raised independent RNA-interference (RNAi) lines of CaPHO1, CaPHO1;like, and CaPHO1;H1 along with triple mutant line in chickpea. While single gene RNAi lines behaved just like WT, triple knock-down RNAi lines (capho1/like/h1) showed reduced shoot growth and shoot Pi content. Lastly, we showed that CaPHO1s are involved in root nodule development and Pi content. Our findings suggest that CaPHO1 members function redundantly in root to shoot Pi export and root nodule development in chickpea. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Masson SAS. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Legumes; Nodulation; PHOSPHATE1 (PHO1); Pi-deficiency; RNAi |
| المشرفين على المادة: | 0 (Plant Proteins) 0 (Phosphates) 0 (Phosphate Transport Proteins) |
| تواريخ الأحداث: | Date Created: 20240511 Date Completed: 20240524 Latest Revision: 20240611 |
| رمز التحديث: | 20240612 |
| DOI: | 10.1016/j.plaphy.2024.108712 |
| PMID: | 38733940 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2690 |
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| DOI: | 10.1016/j.plaphy.2024.108712 |