دورية أكاديمية
أعرض في EDS

Polystyrene microplastics induce male reproductive toxicity in mice by activating spermatogonium mitochondrial oxidative stress and apoptosis.

التفاصيل البيبلوغرافية
العنوان: Polystyrene microplastics induce male reproductive toxicity in mice by activating spermatogonium mitochondrial oxidative stress and apoptosis.
المؤلفون: Fang Q; Department of Anesthesia, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China., Wang C; Department of Pain Management, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China., Xiong Y; Department of Anesthesia, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China. Electronic address: xiongynenene@whu.edu.cn.
المصدر: Chemico-biological interactions [Chem Biol Interact] 2024 Jun 01; Vol. 396, pp. 111043. Date of Electronic Publication: 2024 May 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Ireland NLM ID: 0227276 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7786 (Electronic) Linking ISSN: 00092797 NLM ISO Abbreviation: Chem Biol Interact Subsets: MEDLINE
أسماء مطبوعة: Publication: Limerick : Elsevier
Original Publication: Amsterdam, Elsevier.
مواضيع طبية MeSH: Apoptosis*/drug effects , Oxidative Stress*/drug effects , Microplastics*/toxicity , Polystyrenes*/toxicity , Polystyrenes*/chemistry , Mitochondria*/drug effects , Mitochondria*/metabolism , Mice, Inbred C57BL* , Testis*/drug effects , Testis*/pathology , Testis*/metabolism , Spermatogonia*/drug effects , Spermatogonia*/metabolism , Spermatogonia*/pathology , Membrane Potential, Mitochondrial*/drug effects , Sperm Motility*/drug effects, Animals ; Male ; Mice ; Reactive Oxygen Species/metabolism ; Spermatozoa/drug effects ; Spermatozoa/pathology ; Sperm Count ; Superoxide Dismutase/metabolism
مستخلص: Microplastics have emerged as environmental hazards in recent years. This study was intended to prove the toxic effects of microplastics on the male reproductive system and further elucidate its mechanism. C57bl/6 mice were exposed to ultrapure water or different doses (0.25, 0.5 and 1 mg/d) of 5 μm polystyrene microplastics (PS-MPs) for 4 weeks, and the GC-1 mouse spermatogonium was treated with different concentrations of PS-MPs. The results showed that sperm count and motility were decreased, and sperm deformity rate was increased after exposure to PS-MPs. The morphology of testes in PS-MPs groups exhibited pathological changes, such as abnormal development of spermatogenic tubules, and inhibited spermatogonium function. Furthermore, the fluorescence intensity of TUNEL staining and the BAX/BCL2 ratio were increased. Exposure to PS-MPs resulted in impaired mitochondrial morphology of spermatogonium, decreased activity of GSH-px and SOD, and increased the MDA level. In vitro, after treatment with PS-MPs, the cell apoptosis rate of spermatogonium was significantly increased, mitochondrial membrane potential was decreased, mitochondrial morphology was damaged, and exposure to PS-MPs increased mitochondrial reactive oxygen species, inducing an oxidative stress state in spermatogonia. In summary, PS-MPs induced a decrease in sperm quality by activating spermatogonium mitochondrial oxidative stress and apoptosis, offering novel insights into mitigating the reproductive toxicity of microplastics.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Apoptosis; Microplastics; Oxidative stress; Spermatogonium
المشرفين على المادة: 0 (Microplastics)
0 (Polystyrenes)
0 (Reactive Oxygen Species)
EC 1.15.1.1 (Superoxide Dismutase)
تواريخ الأحداث: Date Created: 20240512 Date Completed: 20240601 Latest Revision: 20240601
رمز التحديث: 20240602
DOI: 10.1016/j.cbi.2024.111043
PMID: 38735450
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-7786
DOI:10.1016/j.cbi.2024.111043