دورية أكاديمية
In vitro ovicidal and larvicidal activity of a hydroalcoholic extract and its fractions from Cyrtocarpa procera fruits on Haemonchus contortus.
| العنوان: | In vitro ovicidal and larvicidal activity of a hydroalcoholic extract and its fractions from Cyrtocarpa procera fruits on Haemonchus contortus. |
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| المؤلفون: | De Jesús-Martínez X; Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad km 1, Ex-Hda. de Aquetzalpa, Tulancingo, C.P. 43600, Hidalgo, Mexico., Rivero-Pérez N; Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad km 1, Ex-Hda. de Aquetzalpa, Tulancingo, C.P. 43600, Hidalgo, Mexico., Zamilpa A; Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No.1 Col, Centro, CP 62790, Xochitepec, Morelos, Mexico., González-Cortazar M; Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No.1 Col, Centro, CP 62790, Xochitepec, Morelos, Mexico. Electronic address: gmanases2000@gmail.com., Olivares-Pérez J; Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Guerrero, Ciudad Altamirano, Guerreo, Mexico., Zaragoza-Bastida A; Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad km 1, Ex-Hda. de Aquetzalpa, Tulancingo, C.P. 43600, Hidalgo, Mexico., Mendoza-de Gives P; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID SAI-INIFAP), Carretera Federal Cuernavaca-Cuautla No. 8534/Col. Progreso, Jiutepec, C.P. 62550, Morelos, Mexico., Villa-Mancera A; Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, Tecamachalco Puebla, Mexico., Olmedo-Juárez A; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID SAI-INIFAP), Carretera Federal Cuernavaca-Cuautla No. 8534/Col. Progreso, Jiutepec, C.P. 62550, Morelos, Mexico. Electronic address: aolmedoj@gmail.com. |
| المصدر: | Experimental parasitology [Exp Parasitol] 2024 Jul; Vol. 262, pp. 108777. Date of Electronic Publication: 2024 May 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370713 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2449 (Electronic) Linking ISSN: 00144894 NLM ISO Abbreviation: Exp Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando, FL : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Haemonchus*/drug effects , Plant Extracts*/pharmacology , Plant Extracts*/chemistry , Larva*/drug effects , Fruit*/chemistry , Anthelmintics*/pharmacology , Anthelmintics*/isolation & purification , Anthelmintics*/chemistry , Ovum*/drug effects, Animals ; Chromatography, High Pressure Liquid ; Sheep ; Haemonchiasis/parasitology ; Haemonchiasis/veterinary ; Sheep Diseases/parasitology |
| مستخلص: | This study describes the in vitro anthelmintic effect of a hydroalcoholic extract (HA-E) and its fractions from Cyrtocarpa procera fruits against Haemonchus contortus eggs and infective larvae. The HA-E was subjected to bipartition using ethyl acetate, which resulted in an aqueous fraction (Aq-F) and an organic fraction (EtOAc-F). The HA-E and both fractions were tested using the egg hatching inhibition assay (EHIA) and the larval mortality test (LMT). Fractionation of the EtOAc-F was achieved using different chromatographic processes, i.e., open glass column and HPLC analysis. Fractionation of the EtOAc-F gave 18 subfractions (C1R1-C1R18), and those that showed the highest yields (C1R15, C1R16, C1R17 and C1R18) were subjected to anthelmintic assays. The HA-E and the EtOAc-F displayed 100% egg hatching inhibition at 3 and 1 mg/mL, respectively, whereas Aq-F exhibited 92.57% EHI at 3 mg/mL. All subfractions tested showed ovicidal effect. Regarding the larval mortality test, HA-E and EtOAc-F exhibited a larvicidal effect higher than 50% at 50 and 30 mg/mL, respectively. The subfractions that showed the highest larval mortality against H. contortus were C1R15 and C1R17, with larval mortalities of 53.57% and 60.23% at 10 mg/mL, respectively. Chemical analysis of these bioactive subfractions (C1R15 and C1R17) revealed the presence of gallic acid, protocatechuic acid, and ellagic acid. This study shows evidence about the ovicidal and larvicidal properties of C. procera fruits that could make these plant products to be considered as a natural potential anthelmintic agents for controlling haemonchosis in goats and sheep. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Anacardiaceae; Anthelmintic; Cyrtocarpa procera; Haemonchus contortus; Phenolic acids |
| المشرفين على المادة: | 0 (Plant Extracts) 0 (Anthelmintics) |
| تواريخ الأحداث: | Date Created: 20240512 Date Completed: 20240614 Latest Revision: 20240614 |
| رمز التحديث: | 20240615 |
| DOI: | 10.1016/j.exppara.2024.108777 |
| PMID: | 38735520 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2449 |
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| DOI: | 10.1016/j.exppara.2024.108777 |